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Dive into the research topics where Catherine Hironaka is active.

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Featured researches published by Catherine Hironaka.


Plant Molecular Biology | 1992

Arabidopsis thaliana small subunit leader and transit peptide enhance the expression ofBacillus thuringiensis proteins in transgenic plants

Edith Wong; Catherine Hironaka; David A. Fischhoff

The expression of the modified gene for a truncated form of thecryIA(c) gene, encoding the insecticidal portion of the lepidopteran-active CryIA(c) protein fromBacillus thuringiensis var.kurstaki (B.t.k.) HD73, under control of theArabidopsis thaliana ribulose-1,5-bisphosphate carboxylase (Rubisco) small subunitats1A promoter with and without its associated transit peptide was analyzed in transgenic tobacco plants. Examination of leaf tissue revealed that theats1A promoter with its transit peptide sequence fused to the truncated CryIA(c) protein provided a 10-fold to 20-fold increase incryIA(c) mRNA and protein levels compared to gene constructs in which the cauliflower mosaic virus 35S promoter with a duplication of the enhancer region (CaMV-En35S) was used to express the samecryIA(c) gene. Transient expression assays in tobacco protoplasts and the whole plant results support the conclusion that the transit peptide plus untranslated sequences upstream of that region are both required for the increase in expression of the CryIA(c) protein. Furthermore, the CaMV-En35S promoter can be used with theArabidopsis ats1A untranslated leader and transit peptide to increase expression of this protein. While subcellular fractionation revealed that the truncated CryIA(c) protein fused to theats1A transit peptide is located in the chloroplast, the increase in gene expression is independent of targeting of the CryIA(c) protein to the chloroplast. The results reported here provide new insight into the role of 5′ untranslated leader sequences and translational fusions to increase heterologous gene expression, and they demonstrate the utility of this approach in the development of insect-resistant crops.


Archive | 2001

DNA molecule for detecting glyphosate tolerant wheat plant 33391 and progeny thereof

Guilan Chen; Catherine Hironaka; Hua-ping Zhou


Archive | 2001

Glyphosate tolerant wheat plant 33391 and compositions and methods for detection thereof

Guilan Chen; Catherine Hironaka; Hua-Ping Zhou


Archive | 2001

Corn transformant PV-ZMGT32 (NK603) and compositions and methods for detection thereof

Carl F Behr; Gregory R. Heck; Catherine Hironaka; Jinsong You


Archive | 2001

Compositions and methods for detection of corn transformant PV-ZMGT32 (NK603)

Carl F Behr; Catherine Hironaka; Gregory R. Heck; Jinsong You


Archive | 2014

Methods for producing glyphosate tolerant plants comprising corn event PV-ZMGT32(nk603)

Carl F Behr; Gregory R. Heck; Catherine Hironaka; Jinsong You


Archive | 2001

Mais-Transformante PV-ZMGT32 (NK603) und Zusammensetzungen und Verfahren zum Nachweis davon

Carl F Behr; Gregory R. Heck; Catherine Hironaka; Jinsong You


Archive | 2001

Zusammensetzungen und Verfahren zum Nachweis von Mais-Transformante PV-ZMGT32 (NK603) Compositions and methods for the detection of corn transformant PV-ZMGT32 (NK603)

Carl F Behr; Catherine Hironaka; Gregory R. Heck; Jinsong You


Archive | 2001

Zusammensetzungen und Verfahren zum Nachweis von Mais-Transformante PV-ZMGT32 (NK603)

Carl F Behr; Catherine Hironaka; Gregory R. Heck; Jinsong You


Archive | 2001

Compositions and methods for detecting majstransformant PV-ZMGT32 (NK603)

Carl F Behr; Gregory R. Heck; Jinsong You; Catherine Hironaka

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