Cecília Cláudia Costa Ribeiro

Low-Fluoride Dentifrice and Caries Lesion Control in Children with Different Caries Experience: A Randomized Clinical Trial

Since there is no consensus on the anticaries effectiveness of low-fluoride (F) dentifrice, this randomized clinical trial evaluated its effect in children at different caries activity status. One hundred and twenty 2- to 4-year-old children, half with and half without active caries lesions, were randomly divided into 2 groups which used 500- or 1,100-µg F/g (NaF) dentifrices during 1 year. Caries progression or regression were evaluated as the number of lesions becoming active/cavities or inactive, respectively. The anticaries effect of the low-F dentifrice was similar to the conventional F dentifrice when used by caries-inactive children. However, in children with active caries lesions the low-F dentifrice was less effective than the 1,100-µg F/g dentifrice in controlling the progression of lesions. The data suggest that the child caries activity may be taken into account to recommend a low-F dentifrice.

Microbiological analysis after complete or partial removal of carious dentin in primary teeth: a randomized clinical trial.

Since viable bacteria can persist in tooth cavities regardless of the technique used for caries removal, the objective of the present randomized clinical trial was to examine the microflora of primary teeth treated by complete or partial removal of carious dentin. Deciduous molars with acute carious lesions in the inner half of dentin and vital pulp were randomly divided into two groups of 16: complete removal, in which the carious dentin was completely removed with the help of a caries detector dye, and partial removal, in which the carious dentin was completely removed from the dentinoenamel junction and lateral walls, while the necrotic carious dentin from the cavity floor was only removed superficially. Dentin samples were obtained with a sterile No. 3 bur after caries removal and 3–6 months after protection with calcium hydroxide cement and restoration of the cavities with resin composite. The samples were stored in thioglycolate. Decimal dilutions were then prepared and seeded for the enumeration of Streptococcus spp., mutans streptococci, Lactobacillus spp. and total microorganisms. Before sealing, a larger number of microorganisms was detected in teeth submitted to partial caries removal compared to the complete removal group. However, after sealing the level of colonization was similar in the two groups for all microorganisms studied. The results suggest that persistence of bacteria does not seem to be a reason for reopening of cavities in deciduous teeth after partial caries removal.

Partial caries removal in primary teeth: association of clinical parameters with microbiological status.

The relationship between clinical characteristics of carious dentin and bacterial colonization after partial caries removal is not completely understood. The aim of this study was to compare microbial counts between categories of carious dentin color, consistency and humidity, and to evaluate the correlation between these characteristics and the presence of cariogenic microorganisms in deep cavities (2/3 or more of the dentin thickness) submitted to partial caries removal. Sixteen primary teeth were submitted to the removal of all carious tissue from the lateral walls of the cavity, whereas carious tissue of the pulp wall was removed superficially. Dentin in the pulp wall was classified according to color, consistency and humidity immediately after cavity preparation and 3–6 months after cavity sealing and a tissue sample was collected on the same occasion for microbiological evaluation. Before sealing, Streptococcus mutans (p = 0.033) and Lactobacillus spp. (p = 0.048) counts were higher in cavities with humid dentin compared to cavities with dry dentin. A negative correlation was observed between carious dentin consistency and S. mutans count during this phase (rs = –0.571; p = 0.020). Arrest of dentinal caries lesions was observed after sealing, which was characterized by a reduction of bacterial counts and changes in dentin color, consistency and humidity, irrespectively of baseline dentin characteristics. The clinical characteristics of carious dentin change after the period of cavity sealing and cannot be applied as absolute indicators to limit the excavation of carious dentin when minimally invasive techniques are used.

Perfil da assistência odontológica pública para a infância e adolescência em São Luís (MA)

This profile of public dental care for children/adolescents in Sao Luis, Maranhao State, Brazil, is based on a survey conducted through interviews, designed to identify these services and help upgrade Health Services available in this city. It describes the pediatric dental care available and the age groups attended, the qualifications of the practitioners involved, the types of treatment for primary and permanent teeth and oral health education programs run at the Municipal Healthcare Units with dental facilities, analyzed through visits and structured interviews. The findings show that dental care was available for children/adolescents (mainly between 6 and 12 years old) at 91.1% of the Healthcare Units offering daily and universal care (65.75%), as well as at those whose services are limited to certain specialties and/or specific days (34.15%). All the public Healthcare Units offered surgical treatment for permanent teeth. Fillings are used more for permanent teeth than primary teeth. Among the public Healthcare Units, 25 (55.5%) did not offer or run oral health programs. At most (75.5%) of the Units visited, dental treatment for children and adolescents is limited to basic care and directed mainly to permanent teeth. The traditional welfare model of providing care as freely demanded remains in place.Descreve existencia de atendimento para criancas, as faixas etarias atendidas, a qualificacao do profissional, os tipos de procedimentos realizados para a denticao decidua e permanente e a existencia de programas educativos de promocao de saude bucal nas Unidades de Saude do Municipio de Sao Luis com assistencia odontologica, por meio de visitas e entrevistas estruturadas. Os resultados mostram que o atendimento odontologico para criancas e adolescentes e disponibilizado em 91,1% das unidades de saude, se concentrando na faixa etaria de 6 a 12 anos, tanto nas unidades que prestam assistencia diaria e universal (65,75%) quanto naquelas (34,15%) onde o atendimento e feito em consultorio restrito, especifico e/ou em dias especificos. Todas as unidades visitadas oferecem tratamento cirurgico para a denticao permanente. O tratamento restaurador e mais disponibilizado para os dentes permanentes do que para os dentes deciduos. Vinte e cinco (55,5%) das unidades de saude nao apresentam ou nao desenvolvem programas de educacao em saude bucal. Na maioria das unidades (75,5%) visitadas, o tratamento odontologico para criancas e adolescentes e restrito a atencao basica e voltado para a denticao permanente. O modelo assistencial tradicional de atendimento de livre demanda permanece.

The effect of iron on Streptococcus mutans biofilm and on enamel demineralization

Iron (Fe) may have an anticaries effect by specific inhibition of glycosyltransferase (GTF) enzymes of Streptococcus mutans, but this hypothesis has not yet been clarified. In this study, S. mutans biofilms were formed on blocks of bovine dental enamel of a predetermined surface hardness (SH). These biofilms were exposed eight times/day to 10% sucrose, and two times/day they were subjected to one of the following treatments: G1, 0.9% NaCl as a negative control; G2, 0.12% chlorhexidine digluconate (CHX) as a positive antibacterial control; G3, 0.05% NaF (225 ppm F) as a positive anticaries control; G4, G5, and G6, ferrous sulfate (Fe2+) at concentrations of 1.0, 10.0, and 100.0 µg Fe/mL, respectively. The experiment was performed in triplicate and was repeated three times (n = 9). The pH of the culture medium was determined every 24 h as an indicator of the biofilms acidogenicity. The biofilm formed on each block was collected for determination of the viable bacteria and concentration of extracellular polysaccharides (EPS). Enamel SH was again determined and the percentage of SH loss (%SHL) was calculated as an indicator of demineralization. Iron treatment reduced the number of viable bacteria formed in the S. mutans biofilm (p = 0.04), in a dose-dependent manner, and also reduced the enamels %SHL (p = 0.005). At 100 µg/mL, Fe reduced enamel demineralization as effectively as CHX and NaF (p < 0.05), but it did not inhibit EPS production. In conclusion, the data suggest that the anticaries mechanism of action of Fe may not involve the oxidative inhibition of GTFs.

Avaliação da fluoretação da água do sistema de abastecimento público na Ilha de São Luís, Maranhão, Brasil

The aim of the present study was to evaluate the fluoride levels in public water supply in Sao Luis island, Maranhao State, Brazil, during the month of October of 2006. A stratification sampling was applied, considering the water treatment station (06) and the different districts (46). For fluoride analysis it was used a specific ion electrode. The fluoride concentration varied for 0.05 to 0.84 ppm. Only one water treatment station (Italuis) presented an ideal concentration of fluoride. It was concluded that the fluoride levels need adjustments, evidencing that it is essential an external control for monitoring of those levels in public water supply of Sao Luis.

Factors underlying the polarization of early childhood caries within a high-risk population

BackgroundEarly childhood caries (ECC) are particularly prevalent in disadvantaged populations, and socioeconomic factors are associated with the polarization of disease. A previous study showed that even within a homogenous low-income population disease is polarized, indicating that other factors apart from income may contribute to disease susceptibility.MethodsThis study used a hierarchical approach to identify factors associated with polarization of ECC in low-income subjects. This cross-sectional study was conducted retrospectively using a cohort of 244 children (aged 48–72 months) with family incomes not exceeding double the minimum wage (U.S.

Relação entre os índices de maturação das vértebras cervicais e os estágios de calcificação dentária

8,208.00/year), living in neighborhoods on the outskirts of São Luís, Maranhão, Brazil. The sample was divided into three groups based on the Significant Caries (SiC) Index: no caries group, few caries group (mean 1.38 lesions), and a high caries group (mean 3.82 lesions). Hierarchical multinomial logistic regression analyses were performed based on a theoretical model.ResultsTwenty-eight (11.5%) of the 244 children presented with high caries. Age (p = 0.026; prevalence ratio (PR) = 1.10; 95% confidence interval (CI) 1.01–1.20) and frequency of sucrose consumption - p = 0.001; PR 4.65 (95% CI 1.83–11.84) were associated with increased risk of ECC.ConclusionsIn the high caries group, greater consumption of sucrose between main meals may explain why, in a group of children with homogenous social and health conditions, some had more caries than others.

Effects of Socioeconomic Status and Social Support on Violence against Pregnant Women: A Structural Equation Modeling Analysis.

OBJETIVO: avaliar a relacao entre os diferentes indices de maturacao ossea das vertebras cervicais e os estagios de calcificacao dentaria, bem como verificar as diferencas existentes entre os generos, em uma amostra de individuos de 10 a 14 anos de idade. METODOS: a amostra consistiu de telerradiografias laterais e radiografias panorâmicas de 464 individuos de ambos os generos, classificadas de acordo com os indices de maturacao das vertebras cervicais descritos por Hassel e Farman (1995) e os estagios de calcificacao descritos por Nolla (1960). RESULTADOS: os resultados obtidos mostraram correlacao entre as variaveis estudadas (p < 0,05). CONCLUSAO: confirmando dados da literatura, observou-se uma precocidade, tanto nos indices de maturacao das vertebras cervicais quanto nos estagios de calcificacao dentaria para o genero feminino em relacao ao genero masculino.

Breastfeeding, Dental Biofilm Acidogenicity, and Early Childhood Caries.

Few studies have used structural equation modeling to analyze the effects of variables on violence against women. The present study analyzed the effects of socioeconomic status and social support on violence against pregnant women who used prenatal services. This was a cross-sectional study based on data from the Brazilian Ribeirão Preto and São Luís birth cohort studies (BRISA). The sample of the municipality of São Luís (Maranhão/Brazil) consisted of 1,446 pregnant women interviewed in 2010 and 2011. In the proposed model, socioeconomic status was the most distal predictor, followed by social support that determined general violence, psychological violence or physical/sexual violence, which were analyzed as latent variables. Violence was measured by the World Health Organization Violence against Women (WHO VAW) instrument. The São Luis model was estimated using structural equation modeling and validated with 1,378 pregnant women from Ribeirão Preto (São Paulo/Brazil). The proposed model showed good fit for general, psychological and physical/sexual violence for the São Luís sample. Socioeconomic status had no effect on general or psychological violence (p>0.05), but pregnant women with lower socioeconomic status reported more episodes of physical/sexual violence (standardized coefficient, SC = -0.136; p = 0.021). This effect of socioeconomic status was indirect and mediated by low social support (SC = -0.075; p<0.001). Low social support was associated with more episodes of general, psychological and physical/sexual violence (p<0.001). General and psychological violence indistinctly affected pregnant women of different socioeconomic status. Physical/sexual violence was more common for pregnant women with lower socioeconomic status and lower social support. Better social support contributed to reduction of all types of violence. Results were nearly the same for the validation sample of Ribeirão Preto except that SES was not associated with physical/sexual violence.