Cecille Labuda

A Theoretical Study of Inertial Cavitation from Acoustic Radiation Force Impulse Imaging and Implications for the Mechanical Index1

The mechanical index (MI) attempts to quantify the likelihood that exposure to diagnostic ultrasound will produce an adverse biological effect by a non-thermal mechanism. The current formulation of the MI implicitly assumes that the acoustic field is generated using the short pulse durations appropriate to B-mode imaging. However, acoustic radiation force impulse (ARFI) imaging employs high-intensity pulses up to several hundred acoustic periods long. The effect of increased pulse durations on the thresholds for inertial cavitation was studied computationally in water, urine, blood, cardiac and skeletal muscle, brain, kidney, liver and skin. The results indicate that, although the effect of pulse duration on cavitation thresholds in the three liquids can be considerable, reducing them by, for example, 6%-24% at 1 MHz, the effect on tissue is minor. More importantly, the frequency dependence of the MI appears to be unnecessarily conservative; that is, the magnitude of the exponent on frequency could be increased to 0.75. Comparison of these theoretical results with experimental measurements suggests that some tissues do not contain the pre-existing, optimally sized bubbles assumed for the MI. This means that in these tissues, the MI is not necessarily a strong predictor of the probability of an adverse biological effect.

Should the mechanical index be revised for ARFI imaging

The mechanical index (MI) quantifies the likelihood that exposure to diagnostic ultrasound will produce an adverse biological effect by a nonthermal mechanism. The current formulation of the MI is based on inertial cavitation thresholds in two liquids, water and blood, as calculated by a formalism assuming very short pulse durations. Although tissue contains a high proportion of water, it is not a liquid but a viscoelastic solid. Further, acoustic radiation force impulse imaging employs high-intensity pulses up to several hundred acoustic periods long. The effect of these differences was studied in water, blood and five representative tissues.

Augmentation of HIFU-induced heating with fibers embedded in a phantom.

The effect of fibers on the rate of heat deposition in the focal region of high-intensity focused ultrasound (HIFU) beams was investigated. Nylon, stainless steel and copper fibers of diameters 0.23-0.25, 0.33 and 0.51-0.53 mm embedded in a phantom were exposed to HIFU. The total energy deposited was quantified by measuring the volumes of the lesions formed. The average volumes of the lesions normalized to the average volume of control lesions were 1.19±0.19, 1.43±0.19 and 2.67±0.21 for increasing nylon fiber diameter, indicating an augmented rate of heating. The maximum normalized volume of lesions at the metal fibers was 0.655. These results are consistent with the material properties, which suggest that the mechanism is increased acoustic absorption along with reduction of heat loss by the nylon fiber. The study supports the possibility of improving the efficacy of HIFU-induced hemostasis in vivo by use of a specially designed, nylon fiber-based medical appliance.

Reciprocity calibration of hydrophones in the Megahertz frequency range [biomedical ultrasound applications]

In biomedical ultrasound, experimental work may be performed at various temperatures whereas hydrophone calibration data are typically specified at only one temperature. It is necessary to know the temperature dependence, if any, of hydrophone sensitivity. Comparison of the results of a three-transducer reciprocity calibration of an Imotec type 80-0.5-4.0 passive PVDF probe at 3/spl deg/C, 15/spl deg/C and 37/spl deg/C to the calibration at 22/spl deg/C show that within the limits of precision of this experiment, there is no difference in hydrophone sensitivity at these four temperatures.

Direct visualization of shear waves in viscoelastic fluid using microspheres

Wormlike micellar fluids, being viscoelastic, support shear waves. Shear waves in 500 mM CTAB-NaSal micellar fluid were visualized by seeding the fluid with 212-250 μm diameter polyethylene microspheres. This method was compared to visualization through birefringence induced by shear stress in the fluid. Measured shear wave speeds were 733 and 722 mm/s, respectively, for each technique. Particle displacement was a sinusoidal function of time and displacement amplitude decreased quadratically with distance from the source. This supports the possibility of using particle amplitude measurements as a measure of attenuation even at low fluid concentration where birefringence visualization techniques fail.

Development of a Tissue-Mimicking Phantom of the Brain for Ultrasonic Studies

Constructing tissue-mimicking phantoms of the brain for ultrasonic studies is complicated by the low backscatter coefficient of brain tissue, causing difficulties in simultaneously matching the backscatter and attenuation properties. In this work, we report on the development of a polyvinyl alcohol-based tissue-mimicking phantom with properties approaching those of human brain tissue. Polyvinyl alcohol was selected as the base material for the phantom as its properties can be varied by freeze-thaw cycling, variations in concentration and the addition of scattering inclusions, allowing some independent control of backscatter and attenuation. The ultrasonic properties (including speed of sound, attenuation and backscatter) were optimized using these methods with talc powder as an additive. It was determined that the ultrasonic properties of the phantom produced in this study are best matched to brain tissue in the frequency range 1-3 MHz, indicating its utility for laboratory ultrasonic studies in this frequency range.

Thermal and spectral behavior of ultrasonically generated shear waves in a viscoelastic micellar fluid

Wormlike micellar (WM) fluids, which flow when subjected to long term stresses, are mechanically viscoelastic over shorter durations. These fluids are birefringent under shear, allowing the study of shear wave propagation using both optical and acoustic modalities. In this work, the temperature and frequency dependent behavior of ultrasonically generated shear waves in a WM fluid is studied. A fluid consisting of hexadecyltrimethylammonium bromide (CTAB) and sodium salicylate (NaSAL) combined in a 5:3 ratio is used in a 200 mM concentration. A high intensity focused ultrasound (HIFU) beam generates radiation pressure in the fluid and can induce shear waves of sufficient amplitude to be visualized optically when the beam is modulated. By pulsing the HIFU beam, a train of shear waves are generated which propagate laterally from the focal region. The temperature and frequency dependent behavior of the HIFU generated shear waves are correlated with the rheological and microstructural properties of the fluid. ...

Temperature and frequency dependent behavior of high intensity focused ultrasound (HIFU)-induced shear waves in a viscoelastic micellar fluid

Wormlike micellar (WM) fluids, which flow when subjected to long term stresses, are mechanically viscoelastic over shorter durations. These fluids are birefringent under shear, allowing the study of shear wave propagation using both optical and acoustic modalities. In this work, the thermal and spectral behavior of ultrasonically generated shear waves in a WM fluid are studied. A fluid consisting of hexadecyltrimethylammonium bromide (CTAB) and sodium salicylate (NaSAL) combined in a 5:3 ratio is used in a 200 mM concentration. A high intensity focused ultrasound (HIFU) beam generates radiation pressure in the fluid and can induce shear waves of sufficient amplitude to be visualized optically when the beam is modulated. By pulsing the HIFU beam, a train of shear waves are generated which propagate laterally from the focal region. The temperature and frequency dependent behavior of the HIFU generated shear waves are correlated with the rheological and microstructural properties of the fluid.Wormlike micellar (WM) fluids, which flow when subjected to long term stresses, are mechanically viscoelastic over shorter durations. These fluids are birefringent under shear, allowing the study of shear wave propagation using both optical and acoustic modalities. In this work, the thermal and spectral behavior of ultrasonically generated shear waves in a WM fluid are studied. A fluid consisting of hexadecyltrimethylammonium bromide (CTAB) and sodium salicylate (NaSAL) combined in a 5:3 ratio is used in a 200 mM concentration. A high intensity focused ultrasound (HIFU) beam generates radiation pressure in the fluid and can induce shear waves of sufficient amplitude to be visualized optically when the beam is modulated. By pulsing the HIFU beam, a train of shear waves are generated which propagate laterally from the focal region. The temperature and frequency dependent behavior of the HIFU generated shear waves are correlated with the rheological and microstructural properties of the fluid.

Development of tissue-mimicking phantom of the brain for ultrasonic studies

Constructing tissue-mimicking phantoms of the brain for ultrasonic studies is complicated by the low backscatter coefficient of brain tissue, causing difficulties in simultaneously matching the backscatter and attenuation properties. In this work, we report on the development of a polyvinyl alcohol (PVA) based tissue-mimicking phantom with properties approaching those of white matter tissue. PVA was selected as the base material for the phantom as its properties can be varied by temperature cycling, variations in concentration and the addition of scattering inclusions, allowing some independent control of backscatter and attenuation. The ultrasonic properties (including speed of sound, attenuation, and backscatter) were optimized using these three methods with talcum powder as a scatterer. It was determined that the ultrasonic properties of the phantom produced in this study are best matched to brain tissue in the frequency range 1.0–2.5 MHz, indicating its utility for benchtop ultrasonic studies in this ...

Computation of ultrasonic pressure fields in feline brain

In 1975, Dunn et al. (JASA 58, 512–514) showed that a simple relation describes the ultrasonic threshold for cavitation-induced changes in the mammalian brain. The thresholds for tissue damage were estimated for a variety of acoustic parameters in exposed feline brain. The goal of this study was to improve the estimates for acoustic pressures and intensities present in vivo during experimental exposures rather than estimating them using linear theory. In our current project, the acoustic pressure waveforms produced in the brains of anesthetized felines were numerically simulated for a spherically focused, nominally f1-transducer (focal length = 13 cm) at increasing values of the source pressure at frequencies of 1, 3, and 9 MHz. The corresponding focal intensities were correlated with the experimental data of Dunn et al. The focal pressure waveforms were also computed at the location of the true maximum. For low source pressures, the computed waveforms were the same as those determined using linear theory...