Celso F. Balthazar

Effect of galactooligosaccharide addition on the physical, optical, and sensory acceptance of vanilla ice cream

The effect of the addition of galactooligosaccharide (GOS) on the physicochemical, optical, and sensory characteristics of ice cream was investigated. Vanilla ice cream was supplemented with 0, 1.5, and 3.0% (wt/wt) GOS and characterized for pH, firmness, color, melting, overrun, as well as subjected to a discriminative sensory test (triangle test). For comparison purposes, ice creams containing fructooligosaccharide were also manufactured. The GOS ice creams were characterized by increased firmness and lower melting rates. Different perceptions were reported in the sensory evaluation for the 3.0% GOS ice cream when compared with the control, which was not observed for the fructooligosaccharide ice cream. Overall, the findings suggest it is possible to produce GOS ice cream with improved stability in relation to the physicochemical parameters and sensory perception.

Physicochemical evaluation of sheep milk yogurts containing different levels of inulin.

The present study aimed to evaluate the physicochemical parameters of sheep milk yogurt smoothies (SMY) containing inulin at different levels (0, 2, 4, and 6%). Titratable acidity and pH, yogurt bacteria counts, fatty acids profile, and healthy lipid indices were evaluated during 28 d of refrigerated storage. As expected for yogurts, Streptococcus thermophilus counts decreased 1 to 3 log cycles and Lactobacillus delbrueckii ssp. bulgaricus counts decreased 1 to 2 cycles from d 1 to 28. The protective effect of inulin on bacteria survival and viability in the food matrix was not verified in the prebiotic SMY during storage, regardless of inulin level. Although lower post-acidification was observed in prebiotic SMY due to inulin addition, no changes were verified in short-chain fatty acids (SCFA) or polyunsaturated fatty acids (PUFA). In contrast, an increase in medium- and long-chain fatty acids (MCFA and LCFA) and monounsaturated fatty acids (MUFA) was observed during storage in all SMY. The most significant levels of fatty acids in SMY were oleic acid, followed by palmitic and myristic acids. A high positive correlation between conjugated linoleic acid (CLA) and oleic acid (r=0.978) was observed. The cis-9,trans-11 CLA isomer represented approximately 78% of total PUFA and 2% of total fatty acids, whereas α-linoleic acid comprised about 22% PUFA and 1% of total fatty acids in SMY. The fatty acid changes during storage were associated with the metabolic activity of the starter bacteria, especially for oleic acid and cis-9,trans-11 CLA isomer. Thus, the SMY represented a great source of these compounds. We observed that inulin levels did not affect fatty acids. A nonsignificant decrease in atherogenic index was observed during storage in all SMY, and a positive correlation (r=0.973) was found between atherogenic index and thrombogenic index of SMY. High correlations were observed between lauric and myristic acids and saturated fatty acids (r=0.907 and r=0.894, respectively), providing evidence of their atherogenic and thrombogenic potential. A negative correlation was observed between stearic acid and atherogenic index (r=-0.612) and between oleic acid and atherogenic index. Sheep milk yogurt could be characterized as a food with low atherogenic and thrombogenic risk because of its healthy lipid composition. Therefore, addition of inulin to SMY could be a good option to improve functionality of this food matrix for dairy companies wishing to enter the functional food market.

Partial substitution of NaCl by KCl and addition of flavor enhancers on probiotic Prato cheese: A study covering manufacturing, ripening and storage time

Cheese is a suitable matrix to deliver probiotic strains but it contains a high amount of sodium. The effect of partial substitution of NaCl by KCl and the addition of flavor enhancers (l-arginine, yeast and oregano extract) on probiotic Prato cheese was investigated after 1, 30, and 60 d of refrigerated storage (immediately after manufacturing, and during ripening and storage). Microbiological (lactic acid bacteria and probiotic Lactobacillus casei 01 counts and survival under gastrointestinal conditions), physicochemical (pH, proteolysis, fatty acids), bioactivity (antioxidant effect and angiotensin I-converting enzyme inhibitory activity), rheological, and water mobility by means of time domain low-field nuclear magnetic resonance were investigated. Significant changes in probiotic survival were observed; however, the sodium reduction and the addition of flavor enhancers did not constitute an obstacle to L. casei 01 (>108 CFU/g) during storage. Slight changes were observed in proteolysis, bioactivity, water mobility, texture profile, and fatty acids of the cheeses as a function of the flavor enhancer added. The sodium reduction and the supplementation of Prato cheese with probiotic cultures may be an effective alternative to the production of a potentially functional cheese.

Assessing the effects of different prebiotic dietary oligosaccharides in sheep milk ice cream

The objective of this study was to assess the effects of different prebiotic dietary oligosaccharides (inulin, fructo-oligosaccharide, galacto-oligossacaride, short-chain fructo-oligosaccharide, resistant starch, corn dietary oligosaccharide and polydextrose) in non-fat sheep milk ice cream processing through physical parameters, water mobility and thermal analysis. Overall, the fat replacement by dietary prebiotic oligosaccharides significantly decreased the melting time, melting temperature and the fraction and relaxation time for fat and bound water (T22) while increased the white intensity and glass transition temperature. The replacement of sheep milk fat by prebiotics in sheep milk ice cream constitutes an interesting option to enhance nutritional aspects and develop a functional food.

The addition of inulin and Lactobacillus casei 01 in sheep milk ice cream

The effect of the Lactobacillus casei 01 and inulin addition on sheep milk ice cream during storage (-18 °C, 150 days) was investigated. Control, probiotic and synbiotic ice cream (10% w/w sheep milk cream; 10% w/w sheep milk cream, L. casei 01, 6 log CFU/mL; 10% w/w inulin, L. casei 01, 6 log CFU/mL, respectively) were manufactured. Microbiological counts (probiotic count, survival after in vitro gastrointestinal resistance, Caco-2 cell adhesion), bioactivity and microstructure were analysed. Physical and textural characteristics, colour parameters, thermal analysis and organic acids/volatile compounds were also evaluated. All formulations supported L. casei 01 viability and maintained above the minimum therapeutic level (>6 log CFU/mL) during storage. Inulin did not affect L. casei 01 survival after the passage through simulated gastrointestinal tract and adhesion to Caco-2 cells while improved the ACE-inhibitory and antioxidant activity. L. casei 01 addition produced several volatile compounds, such as carboxylic acids, alcohols, aldehydes and ketones. Also, scanning electron microscopy showed an interaction between probiotic bacteria and inulin fibre on synbiotic ice cream and the adhesion of L. casei to Caco-2 cells was observed.

Determination of biogenic amines by high-performance liquid chromatography (HPLC-DAD) in probiotic cow's and goat's fermented milks and acceptance

This study evaluated the presence of biogenic amines in fermented cows and goats milks containing probiotic bacteria, during the first 10 days of chilled storage (4 ± 2°C), when the probiotic strains are most viable. The overall acceptance of both fermented milks, produced using the same starter culture and probiotics, was tested. In both products, the initially high levels of tyramine (560 mg kg−1 means for both fermented milks), the predominant biogenic amine, increased during the storage period, which may be considered this amine as a quality index for fermented milks. The other principal biogenic amines (putrescine, cadaverine, histamine, and spermidine) were produced on days 1–5 of storage, and thereafter decreased. At the end of the 10th day, these amines, respectively, showed values of fermented cows milk 20.26, 29.09, 17.97, and 82.07 mg kg−1; and values of fermented goats milk 22.92, 29.09, 34.85, and 53.85 mg kg−1, in fermented cows and goats milk. Fermented cows milk was well accepted compared to fermented goats milk. The results suggested that the content of biogenic amines may be a criterion for selecting lactic acid bacteria used to produce fermented milks.

Sensory evaluation of ovine milk yoghurt with inulin addition

The purpose of this study was to elaborate prebiotic yoghurt using ovine milk and different inulin concentrations (0, 2 and 6%). Samples were submitted to laboratory analysis and two sensory tests with 100 consumers. The results of apparent viscosity and firmness determined by instrumental analysis of each yoghurt and control differed significantly. Just-About-Right showed consistency as the main attribute that varied in treatments. According to penalty analysis, prebiotic yoghurt containing 6% inulin had minor penalties, which indicates its advantage in the quest to better consumer demands. Hence, this treatment demonstrated better results as well as the many prebiotic benefits associated with an adequate fibre amount.

Effect of sodium reduction and flavor enhancer addition on probiotic Prato cheese processing

The effect of partial substitution of NaCl with KCl and the flavor enhancers addition (arginine, yeast extract and oregano extract) on Probiotic Prato cheese processing with (L. casei 01, 7logCFU/mL) was investigated. Microbiological (lactic acid bacteria and probiotic counts), physicochemical (proximate composition, pH, proteolysis), bioactivity (antioxidant and angiotensin I-converting enzyme inhibitory activity), rheological (uniaxial compression and creep tests), water mobility (time domain low field magnetic resonance), microstructure (scanning electron microscopy) and sensory evaluation (consumer test) were performed. Sodium reduction and flavor enhancers addition did not constitute an obstacle to the survival of lactic and probiotic bacteria. Proximate composition, antioxidant and angiotensin I-converting enzyme inhibitory activity, and the rheological parameters were affected by the addition of flavor enhancer. No change in the fatty acid profile of cheeses was observed while good performance in the consumer test was obtained by the addition of yeast extract and oregano extract. Prato cheese can be an adequate carrier of probiotics and the addition of different flavor enhancers can contribute developing this functional product in the cheese industry.

Ultrasound processing of fresh and frozen semi-skimmed sheep milk and its effects on microbiological and physical-chemical quality

The objective of this study was to evaluate the effect of ultrasound treatment on the microbiological quality, protein and free amino acid profile of fresh and frozen stored semi-skimmed sheep milk. Milk was treated as fresh or frozen and stored up to one, three and six months. Output power time and pulse time were the parameters combined to design four different ultrasound (US) treatments: power 78 W and duration 6 min (US1); power 78 W and duration 8 min (US2); power 104 W and duration 4 min (US3) power 104 W and duration 6 min (US4). Pulse duration was of 4 s for each treatment. Sample US1 was discarded due to non effectiveness of US treatment, while other samples showed interesting results. Also, it was verified a frost effect on microorganisms in all samples which were frozen before treatment. No relevant change was reported on amino acid profile. The study showed promising results: the ultrasound treatment inactivated or eliminated the studied contaminant bacteria in semi-skimmed sheep milk, while maintained acceptable amount of lactic bacteria, which could be advantageous for dairy products processing.

Nutritional and functional properties of sheep milk for beneficial dairy products development

Statement of the Problem: Casein phosphopeptides (CPPs) are mineral-binding bioactive peptides derived from enzymatic digestion of casein. They have been shown to increase iron bioavailability due to increasing iron solubility at the alkaline pH of the intestine. Thus, they are potentially applicable as an advantageous preparation for iron fortification of food. This study aimed to characterize a commercially available CPP and determine optimum experimental conditions for binding it with ferrous iron.Peri-natal micronutrient supplementation is commonly recommended to optimize maternal-child health outcomes in Europe. While a healthy diet remains the path of choice for nutrient intake, supplementation is often necessary to ensure adequate micronutrient intake. With the growing preference for non-synthetic ingredients and the increased market for nonanimal-based products, this work describes the process followed for the development of a plant-based multiple micronutrient supplements targeted at women at different stages of their life cycle. A list of key nutrients for inclusion was prioritized, based on the existing literature on nutrient needs for women, as well as on their most pressing wellbeing needs. This was followed by the identification and selection of qualified raw ingredient providers. The final line of products included three multiple-micronutrient supplements meeting the following criteria: vegan, gluten-free, lactose-free, and without artificial conservatives. Microbiological control was carried out three-fold, once by the raw material providers followed by double independent laboratory analysis of the end-product micronutrient supplement. This was then tested for human factor contamination, pesticides, total aerobic microbial count, total yeast count, and total mold count. Emphasis was placed on meeting product standards for the European Union, and specific requirements for the German market in food and pharma. Best practices were observed during the process to achieve multiple quality standard accreditations. In addition, organoleptic properties were adjusted in accordance to the female market demands. The results presented here reach the point of introduction of the newly developed product to the market, with acceptability and effectiveness results to be published at a later date.U of by-products from chicken: Protein hydrolysates of both marine and agricultural origin have many health benefits, including lipid lowering and anti-inflammatory effects. This study aimed to penetrate the effect of protein hydrolysates from chicken (CPHs) on obesity related disease and atherosclerosis. Firstly, a mouse obesity model was used to investigate the effect of four different CPH (CPH1-4) generated using specific hydrolyzation methods. C57BL/6 mice were fed a high-fat control diet (25% w/w casein) or CPHs diets (12.5% w/w CPH and 12.5% w/w casein) for 12 weeks, and plasma lipids, hepatic mitochondrial fatty acid oxidation and plasma cytokine levels was analysed. Diets containing CPH1-4 significantly reduced plasma levels of IL-1β, IL-16, INF-γ, TNFα, and MCP-1. In addition, diets containing CPH3 and 4 increased the hepatic mitochondrial fatty acid oxidation indicating a positive effect on fat burning. Diets with 12.5% w/w CPH3 and 4 were further studied in atherosclerosis-prone apoE-/mice. After 12 weeks of high-fat feeding, CPH4 significantly reduced the atherosclerotic plaque area in the aortic arch compared to control (P<0.02), while CPH3 revealed reduced atherosclerosis at borderline significance ( P<0.09).The plasma level of cholesterol and triacylglycerol remained constant in the two experimental groups, but changed plasma fatty acid composition was observed resulting in an increased plasma level of C20:4n-3 and C20:5n-3 accompanied with increased delta-5 desaturase index and reduced plasma atherogenicity index (AI). Noteworthy, the AI correlated to the plague area. Moreover, the protein hydrolysate from CPH4 tended to decrease plasma levels of inflammatory mediators and a significantly reduced level of MCP-1 was found compared to control. Overall, these data show that protein hydrolysates from chicken, able to increase the mitochondrial fatty acid oxidation, can reduce atherosclerosis and attenuate systemic risk factors related to atherosclerotic disorders, but not directly related to changes in the level of plasma cholesterol.