Cetin Kilinc

Virulence Factors in Staphylococci Isolated From Nasal Cavities of Footballers

Aim: This study aimed to investigate the rate of Panton‐Valentine Leukocidin producing Staphylococcus aureus and methicillin (mecA) and slime (icaA/icaD) genes in staphylococcal strains isolated from nasal cavities of footballers. Materials and Methods: Nasal swab samples were taken from each footballers and a healthy control group for the isolation of staphylococcal strains. The polymerase chain reaction technique was used to determine Panton‐Valentine Leukocidin, mecA and icaA/icaD genes in staphylococcal isolates. Results: Among 91 S. aureus strains, the presence of mecA gene was detected as 9.9%. This ratio was 17.9% (27 of 151) among the coagulase‐negative staphylococci. A significant difference was found between coagulase‐negative staphylococci and S. aureus isolates regarding the presence of mecA gene (P < 0.001). As for the genes of the slime, icaA/icaD genes were detected in 198 of 242 (81.8%) strains. The occurrence of slime genes was 91.2% and 89.4% among the S. aureus coagulase and negative staphylococci, respectively (P > 0.05). There was a statistically significant difference between the frequency of the mecA and slime genes when compared with the healthy control group and the football players (P < 0.01). Of 91 isolates, 22 were found to be methicillin resistant by the oxacillin disc diffusion method, whereas the remaining (220) were methicillin susceptible. Methicillin resistance was detected as 14.9% by the polymerase chain reaction method, whereas it was found as 9.1% by phenotypic methods. Conclusions: Early and accurate diagnosis of virulent staphylococcal strains is crucial because the virulent coagulase‐negative and coagulase‐positive staphylococcal strains in the nasal floras of footballers may be major potential sources of superficial and deep tissue infections.

Does the Intestinal Parasite Enterobius vermicularis Cause Acute Appendicitis

OBJECTIVE Although intestinal parasitic infections rarely cause acute appendicitis, they are common public health problems in undeveloped and developing countries. Parasitic infections should be kept in mind in patients clinically suspected of having acute appendicitis, and treatment procedures should be adopted according to the etiology. METHODS Herein we presented the cases of four patients with clinical findings of acute appendicitis. Patients were clinically suspected of having acute appendicitis, and Enterobius vermicularis was detected in the pathological examinations of specimens. Pinworm infections are common parasitic infections that may mimic appendicitis. RESULTS The pathology of the four cases was noted when the file of 186 patients aged between 4 and 72 years who underwent surgery for acute appendicitis in my hospital was retrospectively reviewed. When the appendectomy specimen was examined histopathologically it was understood that acute appendicitis was caused by Enterobius vermicularis parasite. CONCLUSION In Enterobius infections, performing systemic therapy for patients and their family members is sufficient. To prevent unnecessary appendectomy, this type of infection should be made to ask in the history and clinical findings of patients.

Yoğun bakımda geniş ilaç direnci bulunan Pseudomonas aeruginosa pnömonisi için kolistin tedavisi: olgu sunumu

Pseudomonas aeruginosa kokenleri penisilin baglayan proteinlerde degisiklik, porin mutasyonlari, DNA giraz mutasyonlari ve aktif disa atim mekanizmalari gibi yontemlerle antibiyotiklere direnc mekanizmalari gelistirmektedir. Pseudomonas aeruginosa ozellikle yogun bakim unitelerinde takip edilen hastalarda ciddi enfeksiyonlara yol acmasi ve coklu antibiyotik direnci gostermesi nedeniyle ozel dikkat gosterilmesi gereken bir mikroorganizmadir. Bu raporda, 6 yil paraplejisi oykusu olan 1 haftadir devam eden bulanti, gogus agrisi, oksuruk, balgam, halsizlik, hiriltili solunum, genel durum bozuklugu ile acil servise getirilen 67 yasindaki erkek hasta sunulmustur. Hastada her iki akcigerde orta ve alt zonlara kadar yaygin krepitanraller yaninda CRP yuksekligi, hiperurisemi, akciger grafisinde sag akciger alt zonda konsolide infiltrasyon ile uyumlu opasite artisi, sag akciger ust zonda retikulonoduler tarzda opasite artisi tespit edildi. Derin trakeal aspirat kulturunde gram negatif, oksidaz pozitif, non-fermentatif basil uredigi goruldu. Pseudomonas aeruginosa oldugu tespit edilen basil, disk difuzyon yontemi ile yapilan antibiyogrami neticesinde kolistin disindaki tum antibiyotiklere direncli bulundu. Kolistin tedavisi baslanmasina karsin tedavinin 3. gununde kardiyak arrest olan hasta eksitus kabul edildi. Bu olgu, hastanemizde kolistin haricinde tum antibiyotiklere direnc gosteren Pseudomonas aeruginosa infeksiyonu vakasinin ilk defa gorulmesi ve tedavinin antibiyotik duyarliligina gore duzenlenmesinin vurgulanmasi amaciyla sunuldu.