Chalermraj Wantawin

Effects of oxytetracycline on anammox activity

Batch experiments were conducted to investigate the effects of oxytetracycline on anaerobic ammonium oxidation (anammox) process. The short-and long-term effects on anammox activity were studied by measuring ammonium (NH4 +), nitrite (NO2 −), and nitrate (NO3 −) concentrations over time. Experiments were conducted at NH4 +and NO2 − concentrations of 60–90 mg N/L and 60–190 mg N/L, respectively (NH4 +:NO2 − ratio from 1:1–1:2.25), oxytetracycline concentrations of 10–100 mg/L, and biomass concentrations of 300–800 mg/L. In the short-term study, anammox activity was inhibited by all oxytetracycline concentrations studied. However, daily addition of oxytetracycline to a concentration of 5 ± 3.5 mg/L in the anammox sequencing batch reactor completely inhibited anammox activity in the fifth week. Fluorescent in situ hybridization was used to identify autotrophic ammonium oxidizing bacteria (Nitrosomonas spp., Nitrobacter spp., Nitrospira spp., Candidatus Brocadia anammoxidans, and Candidatus Kuenenia stuttgartiensis). The population of anammox culture was significantly decreased while Nitrosomonas spp. and Nitrospira spp. increased in the fifth week compared with the first week of experiment. A not-competitive model fit the anammox inhibition data at oxytetracycline concentrations of 0–100 mg/L quite well with Vmax of 0.0435 mg N/mg VSS-hr and Ki of 54.66 mg/L.

Fe0-based system as innovative technology for degrading trichloromethane: Redox removal characteristics

Background. The spent waste of aliphatic chlorinated solvents has caused severe deterioration of groundwater quality. Trichloromethane (TCM), which shows health and toxicological effects on human beings, was selected as a model compound to be dechlorinated through a redox system.MethodsThe Fe0-based system including Fe0/H2O, Fe0/UV, Fe0/ H2O2, and Fe0/UV/H2O2, was explored to evaluate its performance in dechlorinating TCM. H2O2 was dosed at later reaction time points to initiate Fenton or photo-Fenton reactions. The first two systems demonstrate the reductive dechlorination of TCM by Fe0-released electrons, while the latter two show dechlorination of TCM by both electron reduction and hydroxyl radical oxidation. The system parameters of TCM remaining, Clbuildup, Fe2+ accumulation, H2O2 residue, and ORP were measured to describe different redox characteristics of TCM dechlorination. The Cl-buildup was used as a way to describe the degree of TCM dechlorination in an open reaction system.ResultsReductive dechlorination efficiencies of TCM were 5% and 6% for the systems of Fe0/H2O and Fe0/UV, respectively. In contrast, the Fe0/H2O, and Fe0/UV/H2O2 systems were capable of dechlorinating TCM reductively and oxidatively by 14% and 15%, respectively. The presence of UV light was found to retard the dissolution of Fe2+, but it enhanced the rate of chloride buildup, based on the comparison of Fe0/H2O and Fe0/UV systems. In addition, UV irradiation plays only a minor role in the Fe0/UV/H2O2 system, in view of TCM dechlorination. Application of small amount of H2O2 results in the increase of Fe2+ accumulation rate in the Fe0/H2O2 system.ConclusionsTCM was dechlorinated mostly through post Fenton oxidation; reductive reaction represents a less efficient way to dechlorinate TCM. The efficiencies of overall TCM dechlorination for the two systems of Fe0/H2O2 and Fe0/UV/ H2O2 are comparable to each other, and this implies that the presence of UV irradiation imposes no significant enhancement.Recommendations and OutlooksIt is highly recommended to initiate effective redox dechlorination of TCM with the system of Fe0/H2O2, where the H2O2 in excess is applied at a later reaction time point.

ANAMMOX-like performances for nitrogen removal from ammonium-sulfate-rich wastewater in an anaerobic sequencing batch reactor

ABSTRACT Ammonium removal by the ANaerobic AMonium OXidation (ANAMMOX) process was observed through the Sulfate-Reducing Ammonium Oxidation (SRAO) process. The same concentration of ammonium (100 mg N L−1) was applied to two anaerobic sequencing batch reactors (AnSBRs) that were inoculated with the same activated sludge from the Vermicelli wastewater treatment process, while nitrite was fed in ANAMMOX and sulfate in SRAO reactors. In SRAO-AnSBR, in substrates that were fed with a ratio of NH4+/SO42− at 1:0.4 ± 0.03, a hydraulic retention time (HRT) of 48 h and without sludge draining, the Ammonium Removal Rate (ARR) was 0.02 ± 0.01 kg N m−3.d−1. Adding specific ANAMMOX substrates to SRAO-AnSBR sludge in batch tests results in specific ammonium and nitrite removal rates of 0.198 and 0.139 g N g−1 VSS.d, respectively, indicating that the ANAMMOX activity contributes to the removal of ammonium in the SRAO process using the nitrite that is produced from SRAO. Nevertheless, the inability of ANAMMOX to utilize sulfate to oxidize ammonium was also investigated in batch tests by augmenting enriched ANAMMOX culture in SRAO-AnSBR sludge and without nitrite supply. The time course of sulfate in a 24-hour cycle of SRAO-AnSBR showed an increase in sulfate after 6 h. For enriched SRAO culture, the uptake molar ratio of NH4+/SO42− at 8 hours in a batch test was 1:0.82 lower than the value of 1:0.20 ± 0.09 as obtained in an SRAO-AnSBR effluent, while the stoichiometric ratio of 1:0.5 that includes the ANAMMOX reaction was in this range. After a longer operation of more than 2 years without sludge draining, the accumulation of sulfate and the reduction of ammonium removal were observed, probably due to the gradual increase in the sulfur denitrification rate and the competitive use of nitrite with ANAMMOX. The 16S rRNA gene PCR-DGGE (polymerase chain reaction-denaturing gradient gel electrophoresis) and PCR cloning analyses resulted in the detection of the ANAMMOX bacterium (Candidatus Brocadia sinica JPN1) Desulfacinum subterraneum belonging to the genus Desulfacinum and bacteria that are involved in sulfur metabolism (Pseudomonas aeruginosa strain SBTPe-001 and Paracoccus denitrificans strain IAM12479) in SRAO-AnSBR.

Comparison of nitrogen removal rates and nitrous oxide production from enriched anaerobic ammonium oxidizing bacteria in suspended and attached growth reactors

Attached growth-systems for the anaerobic ammonium oxidation (anammox) process have been postulated for implementation in the field. However, information about the anammox process in attached growth-systems is limited. This study compared nitrogen removal rates and nitrous oxide (N2O) production of enriched anammox cultures in both suspended and attached growth sequencing batch reactors (SBRs). Suspended growth reactors (SBR-S) and attached growth reactors using polystyrene sponge as a medium (SBR-A) were used in these experiments. After inoculation with an enriched anammox culture, significant nitrogen removals of ammonium (NH4 +) and nitrite (NO2 −) were observed under NH4 +:NO2 − ratios ranging from 1:1 to 1:2 in both types of SBRs. The specific rates of total nitrogen removal in SBR-S and SBR-A were 0.52 mg N/mg VSS-d and 0.44 mg N/mg VSS-d, respectively, at an NH4 +:NO2 − ratio of 1:2. N2O production by the enriched anammox culture in both SBR-S and SBR-A was significantly higher at NH4 +:NO2 − ratio of 1:2 than at NH4 +:NO2 − ratios of 1:1 and 1:1.32. In addition, N2O production was higher at a pH of 6.8 than at pH 7.3, 7.8, and 8.3 in both SBR-S and SBR-A. The results of this investigation demonstrate that the anammox process may avoid N2O emission by maintaining an NH4 +:NO2 − ratio of less than 1:2 and pH higher than 6.8.

Nitrogen removal of anammox cultures under different enrichment conditions.

Anammox bacteria in sludge from an anoxic tank of a municipal wastewater treatment plant at Nongkhaem, Bangkok, Thailand were enriched in two sequencing batch reactors (SBRs; SBR-1 and SBR-2), under different conditions. SBR-1 was open to the atmosphere, while SBR-2 was closed and flushed with a mixture of 95% argon and 5% CO2 during the fill period in order to provide strict anaerobic conditions. The specific nitrogen removal rates of SBR-1 and SBR-2 were 0.43 g N/g VSS-d and 2.59 g N/g VSS-d, respectively. Denaturing gradient gel electrophoresis (DGGE) analyses showed differences in band patterns among the Nongkhaem sludge and the two enrichment cultures. Based on fluorescent in situ hybridization (FISH), the anammox bacteria in both systems were either “Candidatus Brocadia anammoxidans” or “Candidatus Kuenenia stuttgartiensis”. The results from this study demonstrate the potential of alternative anammox systems for nitrogen removal and provide information on the microbial communities of anammox cultures under different enrichment conditions.

Resuscitation of starved suspended- and attached-growth anaerobic ammonium oxidizing bacteria with and without acetate

Anammox application for nutrient removal from wastewater is increasing, though questions remain about anammox resilience to fluctuating conditions. Resuscitation of anammox suspended- and attached-growth cultures after 3 months of starvation was studied with and without acetate dosing. Without acetate, the attached-growth culture recovered more quickly than the suspended-growth culture. Suspended-growth cultures recovered more quickly (within 60 days) with weekly and daily acetate dosing than without, but anammox activity and copy numbers decreased with continued acetate addition. All attached-growth cultures recovered within 60 days, but after that activity with acetate dosing was consistently at least 20% lower than that without acetate addition. Ca. Jettenia caeni, Ca. Anammoxoglobus sp., Ca. Brocadia fulgida, Ca. Brocadia anammoxidans, Ca. Brocadia fulgida and Ca. Jettenia asiatica were identified. Acetate addition can significantly accelerate short-term resuscitation of enriched anammox suspended-growth cultures after starvation but may reduce anammox activity over the longer term in suspended- and attached-growth cultures.

Partial nutrient removal under insufficient organic carbon from digested swine wastewater in sequencing batch biofilm reactor.

The objective of this study was to investigate the possibility of using a biofilm process for partial nutrient removal from digested swine wastewater containing low ratios of chemical oxygen demand (COD) to nitrogen and phosphorus; on average, 1.6 g COD g− 1 N and 7 g COD g− 1 P. We used a laboratory-scale sequencing batch biofilm reactor with alternating conditions of 4 hours anaerobic/ 12 hours aerobic, and a hydraulic retention time of 16 hours. Although the concentration of dissolved oxygen under aerobic conditions was > 2.5 mg L− 1, the efficiency of denitrification was up to 85% of the theoretical maximum at the available influent COD, with an ammonia removal rate of 0.73 g N m− 2 d− 1 and without the accumulation of nitrite or nitrate. Activity tests showed that the biomass from the reactor consisted of denitrifying polyphosphate accumulating organisms (DNPAO) that can use nitrite as an electron acceptor. The organic carbon in the digested swine wastewater was utilized very effectively through the denitrifying phosphorus uptake process, as implied by the low utilization ratios of COD to nitrogen, 4.2 g COD g− 1 N, and phosphorus, 14 g COD g− 1 P. A COD value as low as 50 mg L− 1 and an increased ratio of nitrogen to phosphorus from 4:1 to 6:1 in the effluent, which is more suitable for use as a liquid fertilizer, were achieved through the processes of nitrification and denitrifying phosphorus uptake in the sequencing batch biofilm reactor.