Chanda Turner
Wake Forest Institute for Regenerative Medicine
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Featured researches published by Chanda Turner.
The Journal of Urology | 2010
Weixin Zhao; Tamer Aboushwareb; Chanda Turner; Cathy Mathis; Colleen Bennett; William E. Sonntag; Karl-Erik Andersson; George J. Christ
PURPOSE The prevalence of bladder dysfunctions increases with age. In humans it is difficult to separate changes related to exogenous factors from those directly related to the aging process. Some confounding variables can be avoided by studying age related changes in an animal model. We evaluated the impact of age on bladder function in vivo and in vitro, and characterized the corresponding morphological changes. MATERIALS AND METHODS Young (4 to 6 months old) and old (older than 28 to 30 months) male Fischer/Brown Norway rats were used in the study. Cystometric studies were done in conscious, freely moving rats. After cystometry tissue strips from the bladder body were used in in vitro studies of muscarinic receptor activation and electrical field stimulation, and histological examination. RESULTS Old rats had higher bladder weight than young rats but the bladder-to-body weight ratio did not change. We noted significant age related differences in 8 of 10 cystometric parameters. Old rats had increased bladder capacity, post-void residual volume, micturition volume and frequency, baseline and intermicturition pressure, and spontaneous activity but decreased micturition pressure. Bladder strip responses to carbachol and electrical field stimulation were significantly lower in old than in young rats. Histological examination revealed urothelial thinning, lower muscle mass and higher collagen content in the bladders of old vs young rats. CONCLUSIONS Physiological aging alters bladder function in male rats even when external factors remain constant. Thus, in old rats bladder capacity, post-void residual urine and spontaneous activity are higher, and responses to muscarinic receptor stimulation and electrical field stimulation are lower than in young rats. Such changes correspond to findings in aging human bladders, supporting the view that the Fischer/Brown Norway rat is a useful model in which to study age related bladder function changes.
Neurourology and Urodynamics | 2009
Tamer Aboushwareb; Ge Zhou; Fang Ming Deng; Chanda Turner; Karl-Erik Andersson; Moses Tar; Weixin Zhao; Arnold Melman; Ralph B. D'Agostino; Tung-Tien Sun; George J. Christ
The effects of deleting genes encoding uroplakins II (UPII) and III (UPIIIa) on mouse bladder physiology/dysfunction were studied in male and female wild type and knockout (KO) mice.
The Journal of Urology | 2008
Steve J. Hodges; Ge Zhou; Fang-Ming Deng; Tamer Aboushwareb; Chanda Turner; Karl-Erik Andersson; Peter Santago; Doug Case; Tung-Tien Sun; George J. Christ
PURPOSE Previous study has shown that the absence of uroplakin II can cause urinary tract dysfunction, including vesicoureteral reflux and renal abnormalities, as well as micturition pattern changes. We developed a simple surrogate measure of bladder function using ultraviolet visualization of urinary voiding patterns in a uroplakin II knockout mouse animal model. MATERIALS AND METHODS Three male and 3 female WT mice, and 3 male and 3 female uroplakin II knockout mice were evaluated by cystometric analysis and voiding pattern markings. Voiding pattern markings were graded by independent observers on a scale of 1 to 5 according to the degree of dispersion of voided urine. Statistical analysis was then used to correlate voiding dispersion grades with cystometric parameters in the same mice. RESULTS The degree of dispersion of voiding pattern markings correlated with several measures of bladder function. Specifically the Pearson correlation coefficients for the observed voiding patterns highly correlated with baseline pressure, threshold pressure and intermicturition pressure measurements made during conscious cystometry in these mice (p <0.05). CONCLUSIONS Ultraviolet visualization of urinary voiding patterns of mice correlated well with certain measures of standard cystometric evaluations. As such, this method provides a simple, noninvasive method of evaluating mouse bladder function. Implementation of this methodology, which can potentially be automated for high throughput analysis, can accelerate the development of novel therapy for certain important aspects of bladder disease/dysfunction.
The FASEB Journal | 2008
Timothy A. Bertram; George J. Christ; Belinda J. Wagner; Deepak Jain; Tamer Aboushwareb; John W. Ludlow; Richard Payne; Yagna P. R. Jarajapu; Chanda Turner; Manuel J. Jayo
The Journal of Urology | 2008
Timothy A. Bertram; George J. Christ; Tamer Aboushwareb; Belinda J. Wagner; Deepak Jain; John W. Ludlow; Richard Payne; Yagna Prasada Rao Jarajapu; Chanda Turner; Manuel J. Jayo
ics.org | 2009
Tamer Aboushwareb; Weixin Zhao; Chanda Turner; Cathy Mathis; Colleen Bennett; William E. Sonntag; Karl-Erik Andersson; George J. Christ
European Urology Supplements | 2008
Weixin Zhao; Masood A. Machingal; Chanda Turner; Kelvin J.A. Davies; Arnold Melman; Karl-Erik Andersson; George J. Christ
The Journal of Urology | 2007
Tamer Aboushwareb; Ge Zhou; Chanda Turner; Karl-Erik Andersson; Moses Tar; Arnold Melman; Fang-Ming Deng; Tung-Tien Sun; George J. Christ
The FASEB Journal | 2007
Weixin Zhao; Masood A. Machingal; Chanda Turner; Kelvin J.A. Davies; Karl-Erik Andersson; Arnold Melman; George J. Christ
The FASEB Journal | 2007
Tamer Aboushwareb; Yagna P.R. Jarajapu; Steve Blaha; Steve J. Hodges; Chanda Turner; Weixin Zhao; Karl-Erik Andersson; George J. Christ