Chandrakant D. Pawar

One pot three components microwave assisted and conventional synthesis of new 3-(4-chloro-2-hydroxyphenyl)-2-(substituted) thiazolidin-4-one as antimicrobial agents

A one-pot, three-component, microwave assisted and conventional synthesis of new 3-(4-chloro-2-hydroxyphenyl)-2-(substituted) thiazolidin-4-one (4a-n) was carried out by using N,N-dimethylformamide as a solvent with high product yield. Among these synthesized compounds (4f, 4g, 4l and 4m) were found to be a broad spectrum molecule active against all bacterial and fungus strains tested, except fungus Aspergillus niger. Amongst the compounds (4g, 4l and 4m) were found to be more potent than respective standard drugs used in the experiment against Candida albicans, Staphylococcus aureus and Aspergillus flavus, respectively. All synthesized compounds were also tested for their cytotoxic activity against HeLa and MCF-7 cell lines by the sulforhodamine B (SRB) assay. This study shows that all compounds were non-cytotoxic in nature, and confirmed their antimicrobial specificity apart from any general cytotoxicity.

Synthesis and antimicrobial evaluation of novel ethyl 2-(2-(4-substituted)acetamido)-4-subtituted-thiazole-5-carboxylate derivatives

A series of novel molecules containing thiazole ring structure were designed and synthesized. The structures of the synthesized compounds were elucidated and confirmed by (1)H NMR, (13)C NMR, Mass spectrum and the purity was checked through HPLC analysis. Among these synthesized compounds, 3a-3i and 6a-6c were tested for their antimicrobial activity (minimum inhibitory concentration) against a series of strains of Bacillus subtilis, Staphylococcus aureus and Escherichia coli for antibacterial activity and against the strains of Candida albicans, Aspergillus flavus and Aspergillus niger for antifungal activity respectively. The results of the antimicrobial screening data revealed that most of the tested compounds showed moderate to good microbial inhibitions.

Use of cupric ferrocyanide reagent for the thin-layer chromatographic detection of organophosphate insecticide profenophos

Profenophos is a member of organophosphate insecticide compounds. It is an important insecticide and has a diversifi ed role in agriculture. During the last few years, the Forensic Science Laboratories of Maharashtra, India, detected a large number of human poisoning cases with profenophos. A large number of biological samples were received for toxicological chemical analysis. Thin-layer chromatography (TLC) was the preferred method of choice. By observing the results of the toxicological analyses, the conclusion that profenophos plays a key role can be drawn. Cupric ferrocyanide reagent was found to be a selective spray reagent for profenophos in a routine analysis by TLC. Profenophos reacts with cupric ferrocyanide reagent which gives an intense-pink-colored compound. The other organochlorine insecticides, organ phosphorus insecticides, pyrethroids insecticide as well as visceral constituents (amino acids, peptides, proteins, fats, etc.) do not interfere with the cupric ferrocyanide reagent.

Synthesis and evaluation of [N-(Substituted phenyl)-2-(3-substituted) sulfamoyl) phenyl)] acetamide derivatives as anticancer agents

Abstract A series of molecules containing sulfonyl and amide coupling structure were developed, synthesized and evaluated. Total 21 compounds having sulfonamide and amide groups are synthesized. The structures of the synthesized compounds were elucidated and confirmed by 1H NMR, 13C NMR, Mass spectrum and the purity was checked through HPLC analysis. All synthesized compounds (4a–4u) were tested for their in vitro anticancer activity against a series of different cell lines like A549 (Lung Cancer cell), HeLa (Cervical), MCF-7 (Breast Cancer cell) and Du-145 (Prostate Cancer cell) respectively. The results of the anticancer activity revealed that most of the tested compounds showed moderate to good anticancer activity. Compounds 4d, 4k and 4s show promising anticancer activity in different cell lines.