Chang-Hyen Kim

The Effect of Surface Treatment of Titanium with Sand-Blasting/Acid-Etching or Hydroxyapatite-Coating and Application of Bone Morphogenetic Protein-2 on Attachment, Proliferation, and Differentiation of Stem Cells Derived from Buccal Fat Pad

This study was performed to evaluate the effect of surface treatment of titanium, including: machined (MA), sand-blasted and acid-etched (SA), and hydroxyapatite-coated (HA) surfaces, and application of bone morphogenetic protein-2 (BMP-2) on attachment, proliferation, and differentiation of stem derived from buccal fat pad. Stem cells were isolated from buccal fat pad. Stem cells were cultured on MA, SA, and HA titanium discs with and without BMP-2. Cellular attachment and alkaline phosphatase (ALP) activity were evaluated at day 3, 7, and 14. Osteocalcin expression was performed using immunofluorescent assay. The cells grown on MA surface demonstrated good attachment with spindle-like morphology, and cells grown on SA and HA surfaces demonstrated more stellate-like morphology. An increase of ALP activity was seen in the rough surface (SA and HA groups) and a statistically significant increase was seen in the HA group (p < 0.05). The addition of BMP-2 made an expression of osteocalcin, and the highest expression of osteocalcin was noted in the HA group. Within the limits of this study, surface modification of titanium surfaces by sand-blasting/acid-etching or hydroxyapatite-coating was not able to allow the stem cells to differentiate into osteoblasts without exogenous soluble factors. The addition of BMP-2 showed osteogenic differentiation of stem cells and HA-coated surfaces demonstrated the highest ALP and osteocalcin expression.

Comparative effects of chlorhexidine and essential oils containing mouth rinse on stem cells cultured on a titanium surface

Chlorhexidine (CHX) and Listerine (LIS), an essential oil compound, are the two commonly used adjunctive agents for mechanical debridement, for reducing the bacterial load in the treatment of peri-implant inflammation. However, antimicrobial agents have been reported to be cytotoxic to the alveolar bone cells and gingival epithelial cells. The present study was performed to examine the effects of antiseptics CHX and LIS, on the morphology and proliferation of stem cells. Stem cells derived from the buccal fat pad were grown on machined titanium discs. Each disc was immersed in CHX or LIS for 30 sec, 1.5 min or 4.5 min. Cell morphology was evaluated with a confocal laser microscope and the viability of the cells was quantitatively analyzed with the cell counting kit-8 (CCK-8). The untreated cells attached to the titanium discs demonstrated well-organized actin cytoskeletons. No marked alterations in the cytoskeletal organization were observed in any of the treated groups. The treatment with CHX and LIS of the titanium discs decreased the viability of the cells grown on the treated discs (P<0.05). The stem cells derived from the buccal fat pad were sensitive to CHX and LIS, and a reduction in cellular viability was observed when these agents were applied to the discs for 30 sec. Further studies are required to determine the optimal application time and concentration of this antimicrobial agent for maximizing the reduction of the bacterial load and minimizing the cytotoxicity to the surrounding cells.

Bone Response to Anodized Titanium Implants in Rabbits

Purpose: The quality of implant surface is one of the factors that influence wound healing of implant site and subsequently affect osseointegration. The objective of modification of the surface properties of an implant is to affect the biological consequence. The purpose of this study is to evaluate the biologic response of osseous tissue to anodized implants. Materials and Methods: Two machined titanium implants for control group were installed in a tibia of each rabbit and two anodized implants for test group were installed in the other tibia of each rabbit. At the moment the implants were installed, resonance frequency analysis (RFA) values were measured. After healing periods of 1, 2, 3, and 7 weeks, the implants were uncovered and RFA values were measured again. Removal torque was measured for one implant in the test group and one implant in the control group. Histological evaluation was executed in the other implants. Results: Both of test group and control group have the tendency of greater RFA change rate and removal torque value as healing periods became longer, but were statistically insignificant (P>0.05). However, in the case of the same healing period, the test group tended to have greater RFA change rate and removal torque than the control group (P Conclusion: In summary, the anodized surface showed slightly favorable results and it is postulated that it may facilitate improved stability in bone.