Chang Shik Yin

Hydrogenophaga luteola sp. nov. isolated from reed pond water

A yellowish colored, Gram-staining negative, strictly aerobic, motile, rod-shaped bacterium, designated THG-SQE7T, was isolated from reed pond water in Shangqiu, PR China. Comparative 16S rRNA gene sequence analysis indicated that strain THG-SQE7T is most closely related to Hydrogenophaga pseudoflava ATCC 33668T (98.4xa0%), followed by Hydrogenophaga bisanensis K102T (97.6xa0%) and Hydrogenophaga flava CCUG 1658T (97.6xa0%). DNA–DNA hybridization showed 53.5, 36.0 and 22.5xa0% DNA re-association with H. pseudoflava KCTC 2348T, H. bisanensis KCTC 12980T and H. flava KCTC 1648T, respectively. Chemotaxonomic data revealed that strain THG-SQE7T possesses ubiquinone-8 as the only isoprenoid quinone, summed feature 3 (C16:1ω7c and/or C16:1ω6c), C16:0 and C18:1ω7c as the major fatty acids. The major polar lipids were found to be phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. The DNA G+C content was determined to be 63.7xa0mol%. These data corroborated the affiliation of strain THG-SQE7T to the genus Hydrogenophaga. Thus, the isolate represents a novel species, for which the name Hydrogenophagaluteola sp. nov. is proposed, with THG-SQE7T as the type strain (=KCTC 42501Txa0=xa0CCTCC AB 2014314Txa0=xa0JCM 30433T).

Luteimonas terrae sp. nov., isolated from rhizosphere soil of Radix ophiopogonis.

A Gram-staining-negative, strictly aerobic, rod-shaped and bright-yellow-pigmented bacterium, motile by means of a single polar flagellum, designated THG-MD21T, was isolated from rhizosphere soil of Radix ophiopogonis in Henan province, PR China. On the basis of 16S rRNA gene sequence similarity, strain THG-MD21T belongs to the genus Luteimonas and was most closely related to Luteimonas aestuarii B9T (98.2u2009% sequence similarity), Lysobacter panaciterrae Gsoil 068T (97.2u2009%) and Luteimonas marina FR1330T (97.0u2009%). The DNA G+C content was 64.4u200amol%. The DNA-DNA relatedness between strain THG-MD21T and its closest phylogenetic neighbours was below 30.0u2009%. The only isoprenoid quinone detected in strain THG-MD21T was ubiquinone-8.The major polar lipids were found to be phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol, and the predominant fatty acids were iso-C11u2009:u20090, iso-C15u2009:u20090, iso-C16u2009:u20090, C16u2009:u20090 and iso-C17u2009:u20091ω9c. The DNA-DNA hybridization result and characteristics revealed by a polyphasic study showed that strain THG-MD21T represents a novel species, for which the name Luteimonas terrae sp. nov. is proposed. The type strain is THG-MD21T (u2009=u2009KACC 18131Tu2009=u2009JCM 30122T).

Undibacterium aquatile sp. nov., isolated from a waterfall.

A Gram-stain-negative, aerobic, motile and rod-shaped strain, THG-DN7.3T, was isolated from a waterfall. Strain THG-DN7.3T grew well at 18-28u2009°C and at pHu20096.0-7.5 on Reasoners 2A agar. Based on 16S rRNA gene sequence comparisons, strain THG-DN7.3T was most closely related to Undibacterium jejuense JS4-4T (97.3u2009% 16S rRNA gene sequence similarity) and Undibacterium seohonense SHS5-24T (96.5u2009%). The G+C content of the genomic DNA was 57.4u200amol%. The mean DNA-DNA relatedness of strain THG-DN7.3T with U. jejuense KACC 12607T was 40u2009±u20091u2009% (reciprocal 50u2009±u20092.1u2009%). The major cellular fatty acids of strain THG-DN7.3T were summed feature 3 (C16u2009:u20091ω7c and/or C16u2009:u20091ω6c) (47.4u2009%), C16u2009:u20090 (30.4u2009%), summed feature 8 (C18u2009:u20091ω6c and/or C18u2009:u20091ω7c) (6.8u2009%) and C12u2009:u20090 (6.2u2009%). The predominant isoprenoid quinone was ubiquinone-8. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. The results of the DNA-DNA hybridization and genotypic analysis, in combination with chemotaxonomic and physiological data, demonstrated that strain THG-DN7.3T represents a novel species of the genus Undibacterium, for which the name Undibacterium aquatile sp. nov. is proposed. The type strain is THG-DN7.3T (u2009=u2009KCTC 42243Tu2009=u2009CCTCC AB 2015119T).

Nocardioides flava sp. nov., isolated from rhizosphere of poppy plant, Republic of Korea

AbstractnPhylogenetic and taxonomic characterization was performed for bacterium, designated strain THG-DN5.4T, isolated from the rhizosphere of poppy plant collected from Gyeryongsan, Republic of Korea. Strain THG-DN5.4T consists of Gram-stain-positive, aerobic, non-motile rods. The bacteria grow optimally at 18–30xa0°C, at pH 7.0 and in the presence of 0.5–1.0xa0% NaCl. Based on 16S rRNA gene sequence analysis, strain THG-DN5.4T was found to be most closely related to Nocardioides nitrophenolicus KCTC 047BPT, followed by Nocardioides ginsengisoli KCTC 19135T, Nocardioideskongjuensis KCTC 19054T, Nocardioides simplex KACC 20620T, Nocardioides aromaticivorans KACC 20613T, Nocardioidesdaeguensis KCTC 19799T and Nocardioidescaeni KCTC 19600T. The DNA–DNA relatedness between strain THG-DN5.4T and closely related phylogenetic neighbors was below 45.0xa0%, and the DNA G+C content of strain THG-DN5.4T was 70.8xa0mol%. An isoprenoid quinone was identified as MK-8(H4). Strain THG-DN5.4T was characterized chemotaxonomically as having ll-diaminopimelic acid in the cell-wall peptidoglycan. The polar lipids were identified as diphosphatidylglycerol, phosphatidylglycerol, some unidentified aminolipids and some unidentified polar lipids. iso-C16:0 and C18:1ω9c were identified as the major fatty acids present in THG-DN5.4T. On the basis of a polyphasic taxonomic study, strain THG-DN5.4T is considered to represent a novel species of the genus Nocardioides, for which the name Nocardioides flava sp. nov. is proposed. The type strain is THG-DN5.4T (=KCTC 39606T=CCTCC AB 2015298T).n

Microbacterium horti sp. nov., a bacterium isolated from Cucurbita maxima cultivating soil

A novel bacterial strain THG-SL1T was isolated from a soil sample of Cucurbita maxima garden and was characterized by using a polyphasic approach. Cells were Gram-reaction-positive, non-motile and rod-shaped. The strain was aerobic, catalase positive and weakly positive for oxidase. Phylogenetic analysis based on 16S rRNA gene sequence analysis but it shared highest similarity with Microbacterium ginsengisoli KCTC 19189T (96.6xa0%), indicating that strain THG-SL1T belongs to the genus Microbacterium. The DNA Gxa0+xa0C content of the isolate was 68.9xa0molxa0%. The major fatty acids were anteiso-C15: 0 (39.7xa0%), anteiso-C17: 0 (24.4xa0%) and iso-C16: 0 (18.5xa0%). The major polar lipids of strain THG-SL1T were phosphatidylglycerol (PG) and an unidentified glycolipid (GL). The predominant respiratory isoprenoid quinones were menaquinone-11 and menaquinone-12. The diamino acid in the cell-wall peptidoglycan was ornithine. Based on the results of polyphasic characterization, strain THG-SL1T represented a novel species within the genus Microbacterium, for which the name Microbacterium horti sp. nov. is proposed. The type strain is THG-SL1T (=KACC 18286T=CCTCC AB 2015117T).

Massilia humi sp. nov. isolated from soil in Incheon, South Korea

A Gram-negative, strictly aerobic, non-motile, rod-shaped bacterium, designated THG-S6.8T, was isolated from soil in Incheon, South Korea. Based on 16S rRNA gene sequence, strain THG-S6.8T was moderately related to Massilia plicata 76T (97.3xa0%), Massilia dura 16T (97.2xa0%) and Massilia albidiflava 45T (96.9xa0%). Chemotaxonomic data revealed that strain THG-S6.8T possesses ubiquinone-8 as the predominant respiratory quinone, and summed feature 3 (C16:1ω7c and/or C16:1ω6c) and C16:0 as the major fatty acids. The major polar lipids were found to be phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. The DNA–DNA relatedness between strain THG-S6.8T and Massilia plicata KCTC 12344T and Massilia dura KACC 12342T was 38.7 and 40.5xa0%, respectively. The DNA G+C content was 66.8xa0%. These data, together with phenotypic characterization, suggest the isolate represents a novel species, for which the name Massiliahumi sp. nov. is proposed, with THG-S6.8T as the type strain (=KCTC 42737Txa0=xa0CCTCC AB 2015296T).

Pseudoclavibacter terrae sp. nov. isolated from rhizosphere soil of Ophiopogon japonicus

Strain THG-MD12T, a Gram-reaction-positive, aerobic, non-motile, rod-shaped bacterium was isolated from rhizosphere soil of Ophiopogon japonicus in PR China. THG-MD12T was closely related to members of the genus Pseudoclavibacter and showed the highest 16S rRNA gene sequence similarities with Pseudoclavibacter helvolus KCTC 19531T (98.8u2009%) and Pseudoclavibacter chungangensis KCTC 22691T (96.9u2009%). DNA-DNA hybridization showed 41.9u2009±u20092.1u2009% and 12.4u2009±u20090.9u2009% DNA reassociation with P. helvolus KCTC 19531T and P. chungangensis KCTC 22691T, respectively. Chemotaxonomic analyses revealed that strain THG-MD12T possesses menaquinone-9 as the predominant respiratory quinone, 2,4-diaminobutyric acid as the diamino acid in the peptidoglycan and anteiso-C15u2009:u20090, iso-C16u2009:u20090, C16u2009:u20090 and anteiso-C17u2009:u20090 as the major fatty acids. The polar lipid profile was found to consist of diphosphatidylglycerol, phosphatidylglycerol, two unknown glycolipids and two unknown lipids. These data corroborated the affiliation of THG-MD12T to the genus Pseudoclavibacter. Thus, the isolate represents a novel species, for which the name Pseudoclavibacter terrae sp. nov. is proposed, with THG-MD12T as the type strain (u2009=u2009CCTCC AB 2015124Tu2009=u2009KCTC 39562T).

Chryseobacterium formosus sp. nov., a bacterium isolated from an ancient tree trunk

AbstractnA Gram-reaction-negative, non-motile and rod-shaped bacterium, designated as THG-DN3.6T, was isolated from an ancient tree trunk from Republic of Korea. On the basis of 16S rRNA gene sequence analysis, strain THG-DN3.6T was shown to belong to the genus Chryseobacterium and the highest similarity to Chryseobacteriumindoltheticum LMG 4025T (97.2xa0%) and the closest phylogenetic relatives were Chryseobacterium scophthalmum (96.8xa0%), Chryseobacterium piscium (96.7xa0%) and Chryseobacterium balustinum KCTC 2903T (96.3xa0%). The DNA Gxa0+xa0C content of the isolate was 33.2xa0molxa0%. The predominant isoprenoid quinone was menaquinone-6. The major fatty acids were iso-C15:0, summed feature 3 (C16:1ω7c and/or C16:1ω7t and/or iso-C15:0 2-OH), iso-C17:1ω9c and iso-C17:0 3-OH. The major polar lipids of strain THG-DN3.6T were phosphatidylethanolamine. The mean DNA–DNA relatedness of strain THG-DN3.6T to C.indoltheticum LMG 4025T was 52xa0±xa00.5xa0%. Based on the results of polyphasic characterization, strain THG-DN3.6T represented a novel species within the genus Chryseobacterium, for which the name Chryseobacterium formosus sp. nov. is proposed. The type strain is THG-DN3.6T (=KCTC 42606xa0=xa0CCTCC AB 2015118). The NCBI GenBank accession number for the 16S rRNA gene sequence of strain THG-DN3.6T is KM035938.n

Sphingobium soli sp. nov. isolated from rhizosphere soil of a rose

Strain THG-SQA7T, a Gram-negative, strictly aerobic, non-motile, rod-shaped bacterium was isolated from rhizosphere soil of a rose in PR China. Strain THG-SQA7T is closely related to the members of the genus Sphingobium, showing the highest 16S rRNA gene sequence similarities with Sphingobiumlactosutens KACC 18100T (98.2xa0%) and Sphingobium abikonense KCTC 2864T (98.1xa0%). The DNA–DNA relatedness between strain THG-SQA7T and S. lactosutens KACC 18100T and S. abikonense KCTC 2864T was 26.2xa0±xa00.9 and 28.3xa0±xa01.2xa0%, respectively. Chemotaxonomic data showed that strain THG-SQA7T possesses ubiquinone Q-10 as the predominant respiratory quinone, and C18:1ω7c, C16:0, summed feature 3 (C16:1ω7c and/or C16:1ω6c) and C14:0 2OH as the major fatty acids. The major polar lipids were found to be phosphatidylethanolamine, phosphatidylmonomethylethanolamine, phosphatidylglycerol, sphingoglycolipid, diphosphatidylglycerol and phosphatidyldimethylethanolamine. Based on these results, together with phenotypic characterization, a novel species, Sphingobiumsoli sp. nov. is proposed.with the type strain is THG-SQA7T (=CCTCC AB 2015125Txa0=xa0KCTC 42607T).

Sphingosinicella cucumeris sp. nov., isolated from soil of a cucumber garden

A novel bacterial strain, THG-sc1T, was isolated from a soil sample of a cucumber garden and was characterised by using a polyphasic approach. Cells were observed to be Gram-stain negative, non-motile and rod-shaped. The strain was found to be aerobic, catalase and oxidase positive, esculin and starch negative, and to have an optimum growth temperature and pH of 28xa0°C and 7.5, respectively. On the basis of 16S rRNA gene sequence analysis, strain THG-sc1T was found to belong to the genus Sphingosinicella and to be closely related to Sphingosinicella vermicomposti KCTC 22446T, followed by Sphingosinicella xenopeptidilytica DSM 17130T and Sphingosinicella microcystinivorans KCTC 12019T. The DNA G+C content was determined to be 60.8xa0mol% and the predominant respiratory quinone was identified as ubiquinone-10. The major polyamine was found to be sym-homospermidine. The major polar lipids were identified as sphingoglycolipid, phosphatidylethanolamine, phosphatidylglycerol and an unidentified phospholipid. The major fatty acids were identified as C18:1ω7c, C16:0 and summed feature 3 (C16:1ω7c and/or iso-C15:0 2-OH, as defined by MIDI). The results of the genotypic analysis, in combination with chemotaxonomic and physiological data, demonstrated that strain THG-sc1T represents a novel species within the genus Sphingosinicella, for which the name Sphingosinicella cucumeris is proposed. The type strain is THG-sc1T (=KACC 18279Txa0=xa0CCTCC AB 2015120T).