Changcheng Wang

Selective detection of 5-formyl-2'-deoxycytidine in DNA using a fluorogenic hydroxylamine reagent.

Fluorogenic hydroxylamine reagents were used for detecting 5-fC through a labeling pathway. Chemical synthesis, HPLC, denaturing PAGE, and DNA MS were applied to testify that the probe reacted with 5-fC with oligodeoxynucleotide selectivity to achieve 5-fC detection conveniently and quantificationally with the method of fluorescence. The feasibility of fluorescently detecting 5-fC in a genome was also investigated.

A two-photon fluorescent probe for intracellular detection of tyrosinase activity.

AAN effective sensor: The two-photon turn-on fluorescent probe NHU was synthesized to optically detect tyrosinase activity in vitro and in melanoma cells. NHU is composed of a 4-aminophenol moiety and a naphthylamine unit, both of which are connected through a urea linkage. Upon exposure to tyrosinase, the 4-aminophenol site is gradually oxidized to the corresponding orthoquinone, ultimately releasing the highly fluorescent product 6-acyl-N-methyl-2-naphthylamine (AAN).

A turn-on fluorescent probe for detection of tyrosinase activity

We have presented a fluorescent probe that exhibits a fluorescence turn-on signal upon reaction with tyrosinase, and we show that it can be readily employed for the assessment of tyrosinase activity and tyrosinase inhibitor activities in buffered aqueous solution.

A selective turn-on fluorescence strategy for the detection of 5-hydroxymethyl-2′-deoxycytidine

A new type of 1,3-O,N-heterocycle, including two fluorescent derivatives, has been synthesised by the reaction of the hydroxyl and amine groups of 5-hydroxymethyl-2′-deoxycytidine (hmC). This reactivity is shown to enable the selective recognition of hmC over other natural nucleotides in aqueous buffer.