Changqin Zhu

A flow-injection chemiluminescence method for the determination of some estrogens by enhancement of luminol-hydrogen peroxide-tetrasulfonated manganese phthalocyanine reaction.

A novel flow-injection chemiluminescence (FI-CL) method for the determination of estrogens is proposed, based upon its enhancing effect on the CL reaction of luminol with hydrogen peroxide catalyzed by tetrasulfonated manganese phthalocyanine (MnTSPc) in alkaline solution. Under the selected experimental conditions, a linear relationship was obtained between the CL intensity and the concentration of estrone in the range of 1.0x10(-7) to 1.0x10(-6)mol/l, estradiol in the range of 9.0x10(-8) to 1.0x10(-6)mol/l and estriol in the range of 3.0x10(-7) to 2.0x10(-6)mol/l, respectively. The detection limits were 5.1x10(-8)mol/l for estrone, 7.2x10(-9)mol/l for estradiol and 6.5x10(-8)mol/l for estriol with a relative standard deviation of 2.8% for 5.0x10(-7)mol/l estrone, 2.4% for 1.0x10(-7)mol/l estradiol, and 3.1% for 7.0x10(-7)mol/l estriol (n=11). This method has been applied to the determination of estrogen in pharmaceutical injections and tap water with satisfactory results.

APPLICATION OF MANGANESE-TETRASULFONATOPHTHALOCYANINE AS A NEW MIMETIC PEROXIDASE IN THE DETERMINATION OF HYDROGEN PEROXIDE BY CHEMILUMINESCENCE REACTION WITH LUMINOL

A new promising mimetic peroxidase, manganese-tetra-sulfonatophthalocyanine (MnTSPc) has been synthesized and applied to the determination of hydrogen peroxide based on its catalytic effect on the chemiluminescence reaction between luminol and hydrogen peroxide. Under optimum conditions, the calibration graph has a linear range of 4.0 × 10−8–2.0 × 10−5 mol/l hydrogen peroxide, the detection limit of the method is 6.8 × 10−9 mol/l. At a hydrogen peroxide concentration of 1.0 × 10−6 mol/l, the relative standard deviation is 2.4% (n = 10).

Determination of Microamounts of Proteins by Resonance Light Scattering with Copper Phthalocyanine Tetrasulfonic Acid

Abstract Based on the strong enhancement effect of proteins on the resonance light scattering of copper phthalocyanine tetrasulfonic acid, a method for the determination of microamounts of proteins has been developed. Under the experimental conditions (2.0×10−6 mol/L copper phthalocyanine tetrasulfonic acid, pH 2.60, ionic strength 0.001 mol/L NaCl), the linear range of this assay is 0.06–4.0 µg/mL for bovine serum albumin (BSA), 0.1–2.0 µg/mL for human serum albumin (HSA), 0.0–2.0 µg/mL for human γ‐IgG, and 0.2–6.0 µg/mL for ovalbumin. The detection limits (3δ) are 16.8 ng/mL for BSA, 23.4 ng/mL for HSA, 37.6 ng/mL for human γ‐IgG, and 48.3 ng/mL for ovalbumin, respectively. This method has been applied to the analysis of total proteins in human serum samples collected from the hospital, and the results were in good agreement with those reported by the hospital.