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Dive into the research topics where Charlotte Grenade is active.

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Featured researches published by Charlotte Grenade.


Journal of Prosthetic Dentistry | 2011

Fit of single tooth zirconia copings: comparison between various manufacturing processes

Charlotte Grenade; Amélie Mainjot; Alain Vanheusden

STATEMENT OF PROBLEM Various CAD/CAM processes are commercially available to manufacture zirconia copings. Comparative data on their performance in terms of fit are needed. PURPOSE The purpose of this in vitro study was to compare the internal and marginal fit of single tooth zirconia copings manufactured with a CAD/CAM process (Procera; Nobel Biocare) and a mechanized manufacturing process (Ceramill; Amann Girrbach). MATERIAL AND METHODS Abutments (n=20) prepared in vivo for ceramic crowns served as a template for manufacturing both Procera and Ceramill zirconia copings. Copings were manufactured and cemented (Clearfil Esthetic Cement; Kuraray) on epoxy replicas of stone cast abutments. Specimens were sectioned. Nine measurements were performed for each coping. Over- and under-extended margins were evaluated. Comparisons between the 2 processes were performed with a generalized linear mixed model (α=.05). RESULTS Internal gap values between Procera and Ceramill groups were not significantly different (P=.13). The mean marginal gap (SD) for Procera copings (51(50) μm) was significantly smaller than for Ceramill (81(66) μm) (P<.005). The percentages of over- and under-extended margins were 43% and 57% for Procera respectively, and 71% and 29% for Ceramill. CONCLUSIONS Within the limitations of this in vitro study, the marginal fit of Procera copings was significantly better than that of Ceramill copings. Furthermore, Procera copings showed a smaller percentage of over-extended margins than did Ceramill copings.


Journal of Oral Implantology | 2015

Less invasive surgical procedures using narrow diameter implants: a prospective study in 20 consecutive patients.

Geoffrey Lecloux; Charlotte Grenade; Alice Bouhy; Marc Lamy; Eric Rompen

Narrow-diameter implants (NDIs) are increasingly produced and used in implant dentistry, especially since the introduction of new, more resistant materials. The objective of the present study was to evaluate the clinical performance of NDIs (3.3 mm) placed in thin alveolar crests. Twenty consecutive patients needing implant-supported fixed partial dentures and presenting an alveolar thickness ≤6 mm were treated with 1 or several NDIs. The surgical protocol was chosen according to the clinical situation: (1) flapless, (2) mini-cervical flap, (3) wide flap, (4) wide flap + guided bone regeneration (GBR). Implants were immediately loaded if the primary stability was higher than 20 Ncm. Implant survival and success, prosthodontic success rates, and patient-centered outcomes were evaluated after a follow-up period of 1 year. A total of 39 implants were placed in 20 patients, 12 and 27 implants in the anterior regions and in the posterior mandible, respectively. All but 1 implant reached an insertion torque higher than 20 Ncm and were loaded within 48 hours. The implant survival and success rates both reached 94.7%. The need for GBR was avoided in 60% of the implant sites. The mean peri-implant bone remodeling after a follow-up period of 1 year was -0.35 mm at the implant level. Peri-implant bone remodeling was higher in the posterior region, when the alveolar crest was thinner than 4 mm and GBR was required in addition. In conclusion, use of NDIs to restore partial edentation in sites with limited horizontal thickness seems to be an effective treatment option that prevented GBR in the majority of the present cases. Immediate provisionalization of NDIs does not seem to impair the results.


Journal of Dentistry | 2017

No post-no core approach to restore severely damaged posterior teeth: An up to 10-year retrospective study of documented endocrown cases

Marcia M. Belleflamme; Sabine Geerts; Marie M. Louwette; Charlotte Grenade; Alain Vanheusden; Amélie Mainjot

OBJECTIVES The objectives of the present study were to (1) retrospectively evaluate documented cases of ceramic and composite endocrowns performed using immediate dentin sealing (IDS); (2) correlate failures with clinical parameters such as tooth preparation characteristics and occlusal parameters. METHODS 99 documented cases of endocrowns were evaluated after a mean observation period of 44.7±34.6months. A classification of restorations was established in function of the level of damage of residual tooth tissues after preparation, from 1 to 3. Evaluation was performed according to FDI criteria and endodontic outcomes were analyzed. Occlusal risk factors were examined and fractographic analysis was performed in case of fracture. RESULTS 48.4% of patients were shown to present occlusal risk factors. 75.8% of restorations were Class 3 endocrowns. 56.6% were performed on molars, 41.4% on premolars and 2.0% on canines. 84.8% were performed in lithium-disilicate glass-ceramic and 12.1% in Polymer-Infiltrated Ceramic Network (PICN) material. The survival and success rates of endocrowns were 99.0% and 89.9% respectively, while the 10-year Kaplan-Meier estimated survival and success rates were 98.8% and 54.9% respectively. Ten failures were detected: periodontal disease (n=3), endocrown debonding (n=2), minor chipping (n=2), caries recurrence (n=2) and major fractures (n=1). Due to the reduced amount of failures, no statistical correlation could be established with clinical parameters. CONCLUSIONS Endocrowns were shown to constitute a reliable approach to restore severely damaged molars and premolars, even in the presence of extensive coronal tissue loss or occlusal risk factors, such as bruxism or unfavorable occlusal relationships. CLINICAL SIGNIFICANCE Practitioners should consider the endocrown instead of the post and core approach to restore severely damaged non-vital posterior teeth. This minimally invasive solution reduces the risk of catastrophic failures and is easily performed. The use of IDS procedure and lithium-disilicate glass-ceramic as prosthesis material gave very good results.


Dental Materials | 2017

Biocompatibility of polymer-infiltrated-ceramic-network (PICN) materials with Human Gingival Keratinocytes (HGKs)

Charlotte Grenade; Marie-Claire De Pauw-Gillet; Catherine Pirard; Virginie Bertrand; Corinne Charlier; Alain Vanheusden; Amélie Mainjot

OBJECTIVE Biocompatibility of polymer-infiltrated-ceramic-network (PICN) materials, a new class of CAD-CAM composites, is poorly explored in the literature, in particular, no data are available regarding Human Gingival Keratinocytes (HGK). The first objective of this study was to evaluate the in vitro biocompatibility of PICNs with HGKs in comparison with other materials typically used for implant prostheses. The second objective was to correlate results with PICN monomer release and indirect cytotoxicity. METHODS HGK attachment, proliferation and spreading on PICN, grade V titanium (Ti), yttrium zirconia (Zi), lithium disilicate glass-ceramic (eM) and polytetrafluoroethylene (negative control) discs were evaluated using a specific insert-based culture system. For PICN and eM samples, monomer release in the culture medium was quantified by high performance liquid chromatography and indirect cytotoxicity tests were performed. RESULTS Ti and Zi exhibited the best results regarding HGK viability, number and coverage. eM showed inferior results while PICN showed statistically similar results to eM but also to Ti regarding cell number and to Ti and Zi regarding cell viability. No monomer release from PICN discs was found, nor indirect cytotoxicity, as for eM. SIGNIFICANCE The results confirmed the excellent behavior of Ti and Zi with gingival cells. Even if polymer based, PICN materials exhibited intermediate results between Ti-Zi and eM. These promising results could notably be explained by PICN high temperature-high pressure (HT-HP) innovative polymerization mode, as confirmed by the absence of monomer release and indirect cytotoxicity.


Dental Materials | 2016

Biocompatibility of polymer-infiltrated-ceramic-network (PICN) materials with Human Gingival Fibroblasts (HGFs).

Charlotte Grenade; Marie-Claire De Pauw-Gillet; Patrick Gailly; Alain Vanheusden; Amélie Mainjot

OBJECTIVES Polymer-infiltrated-ceramic-network (PICN) materials constitute an innovative class of CAD-CAM materials offering promising perspectives in prosthodontics, but no data are available in the literature regarding their biological properties. The objective of the present study was to evaluate the in vitro biocompatibility of PICNs with human gingival fibroblasts (HGFs) in comparison with materials typically used for implant prostheses and abutments. METHODS HGF attachment, proliferation and spreading on discs made of PICN, grade V titanium (Ti), yttrium zirconia (Zi), lithium disilicate glass-ceramic (eM) and polytetrafluoroethylene (negative control), were evaluated using a specific insert-based culture system (IBS-R). Sample surface properties were characterized by XPS, contact angle measurement, profilometry and SEM. RESULTS Ti and Zi gave the best results regarding HGF viability, morphology, number and coverage increase with time in comparison with the negative control, while PICN and eM gave intermediate results, cell spreading being comparable for PICN, Ti, Zi and eM. Despite the presence of polymers and their related hydrophobicity, PICN exhibited comparable results to glass-ceramic materials, which could be explained by the mode of polymerization of the monomers. SIGNIFICANCE The results of the present study confirm that the currently employed materials, i.e. Ti and Zi, can be considered to be the gold standard of materials in terms of HGF behavior, while PICN gave intermediate results comparable to eM. The impact of the present in vitro results needs to be further investigated clinically, particularly in the view of the utilization of PICNs for prostheses on bone-level implants.


Cytotechnology | 2016

A new method using insert-based systems (IBS) to improve cell behavior study on flexible and rigid biomaterials.

Charlotte Grenade; Nicolas Moniotte; Eric Rompen; Alain Vanheusden; Amélie Mainjot; Marie-Claire De Pauw-Gillet


Journal of Dental Research | 2018

One-Step No-Prep Treatment of Worn Dentition using PICNs

Julie Oudkerk; Maher Eldafrawy; Sandrine Bekaert; Charlotte Grenade; Alain Vanheusden; Amélie Mainjot


European Cells & Materials | 2017

One-step and no-prep treatment of worn dentition using PICNs. A prospective clinical study

Julie Oudkerk; Maher Eldafrawy; Sandrine Bekaert; Charlotte Grenade; Alain Vanheusden; Amélie Mainjot


Archive | 2014

In vitro evaluation of HGF and HGK attachment and proliferation on prostheses materials: a pilot study

Charlotte Grenade; Amélie Mainjot; Alain Van Heusden; Eric Rompen; Marie-Claire Gillet


Dental Materials | 2013

A new in vitro protocol to study HGF/HGK attachment and proliferation on prostheses materials

Charlotte Grenade; Amélie Mainjot; Alain Vanheusden; Eric Rompen; Marie-Claire Gillet

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