Che Dai-di

Study on Mutant Induction of Gladiolus by in vitro Culture of Petals

Petals of Gladiolus ”Rose Supreme” were used to establish regeneration system. Developmental characteristics of petals were observed. A total of 94 plantlets of petal somaclonal line were marked in M1 generation by ISSR molecular markers and traits were observed in M3 generation. The results showed that the best medium to induce callus was MS+2, 4-D 4.0 mg˙L^(-1)+6-BA 0.5 mg˙L^(-1) and the best medium to induce somatic embryogenesis was MS+2, 4-D1.0 mg˙L^(-1)+TDZ0.3 mg˙L^(-1). New petals could be formed from petal callus directly. Pigments of mother plant appeared on plantlets while they were formed from petals. Two mutants were obtained from petal somaclonal line in M3 generation which demonstrated the correctness of ISSR analysis in M1 generation.

Components of Flower Pigments in Petals of Lily

Abstract This paper carried on a preliminary study on pigment composition of lilies of different colors by means of specific color reactions and UV-visible spectra. The results showed that the colors of lilies were usually caused by the combined action of several pigments; yellow and orange lilies mainly contained the flavonoids and carotenoids; pink lilies mainly contained flavonoids and anthocyanins, and white lilies contained small amount of flavonoids.

ISSR Analysis of Hybrid Descendants of Roses

Abstract Four different rose hybrid populations were analyzed by ISSR technique with nine polymorphic primers. All of the primers produced 64 bands, of which 59 were polymorphic. The average of polymorphic bands was 6.6 for each primer. The percentage of polymorphism ranged from 57.1% to 100%, with an average of 92.3%. An UPGMA dendrogram showed five groups. The primer numbered with PI9 and PI11 had relatively high resolution in identifying the F 1 offspring in this research.