Chenggang Liu

Photoprotective and antioxidative mechanisms against oxidative damage in Fargesia rufa subjected to drought and salinity

Drought and salinity are the two most common and frequently co-occurring abiotic stresses limiting plant productivity worldwide, yet it remains unclear whether bamboo species possess effective mechanisms to protect against oxidative damage caused by drought and salinity, either alone or in combination. In this study, we utilised Fargesia rufa Yi, a species important to forest carbon sequestration and endangered giant pandas, to evaluate physiological, biochemical and ultrastructural responses to drought, salinity and their combination. Under drought alone, F. rufa exhibited reduced water loss from leaves, photochemistry inhibition, pigment degradation, reactive oxygen species accumulation, lipid peroxidation, and damage to organelles compared with salinity and combined stress treatments. The superior performance under drought alone was attributed to greater thermal dissipation and the water-water cycle capacities, increased SOD/AsA-GSH cycle enzymes activities, and a favourable redox balance of antioxidants. Therefore, relative to salinity alone and drought+salinity, F. rufa plants under drought exhibit highly efficient mechanisms to protect against oxidative damage, which most likely allow accelerated recovery of photosynthetic plasticity once the stress is removed.

Phosphorous Application Improves Drought Tolerance of Phoebe zhennan

Phoebe zhennan (Gold Phoebe) is a threatened tree species in China and a valuable and important source of wood and bioactive compounds used in medicine. Apart from anthropogenic disturbances, several biotic constraints currently restrict its growth and development. However, little attention has been given to building adaptive strategies for its conservation by examining its morphological and physio-biochemical responses to drought stress, and the role of fertilizers on these responses. A randomized experimental design was used to investigate the effects of two levels of irrigation (well-watered and drought-stressed) and phosphorous (P) fertilization treatment (with and without P) to assess the morphological and physio-biochemical responses of P. zhennan seedlings to drought stress. In addition, we evaluated whether P application could mitigate the negative impacts of drought on plant growth and metabolism. Drought stress had a significant negative effect on the growth and metabolic processes of P. zhennan. Despite this, reduced leaf area, limited stomatal conductance, reduced transpiration rate, increased water use efficiency, enhanced antioxidant enzymes activities, and osmolytes accumulation suggested that the species has good adaptive strategies for tolerating drought stress. Application of P had a significant positive effect on root biomass, signifying its improved water extracting capacity from the soil. Moreover, P fertilization significantly increased leaf relative water content, net photosynthetic rate, and maximal quantum efficiency of PSII under drought stress conditions. This may be attributable to several factors, such as enhanced root biomass, decreased malondialdehyde content, and the up-regulation of chloroplast pigments, osmolytes, and nitrogenous compounds. However, P application had only a slight or negligible effect on the growth and metabolism of well-watered plants. In conclusion, P. zhennan has a strong capability for drought resistance, while P application facilitates and improves drought tolerance mostly through physio-biochemical adjustments, regardless of water availability. It is imperative to explore the underlying metabolic mechanisms and effects of different levels of P fertilization on P. zhennan under drought conditions in order to design appropriate conservation and management strategies for this species, which is at risk of extinction.

The Synergistic Responses of Different Photoprotective Pathways in Dwarf Bamboo (Fargesia rufa) to Drought and Subsequent Rewatering

Dwarf bamboo-dominated forests are often subjected to temporary periods of drought due to rising air temperature and decreasing rainfall. Nevertheless, the relationship among CO2 assimilation, photoprotective pathways and metabolism of reactive oxygen species (ROS) remains unexplored in bamboo species. Changes in leaf gas exchange, chlorophyll fluorescence, energy partitioning, antioxidative system and compounds related to ROS metabolism in Fargesia rufa plants subjected to drought and subsequent rewatering were analyzed. Drought resulted in a reversible inhibition of photochemistry, particularly net CO2 assimilation, and lipid peroxidation due to ROS accumulation. Meanwhile, photoprotective pathways, including the water–water cycle (especially for moderate drought), and adjustment in antenna pigments, thermal dissipation and antioxidative defense capacity at organelle levels (especially for severe drought), were up-regulated at the stress phase. Conversely, photorespiration was down-regulated after drought stress. As a result, rewatering restored most of the photochemical activity under drought, especially moderate drought. Moreover, thermal dissipation under severe drought was still operated for avoiding high ROS levels after rewatering. Therefore, the synergistic function of these photoprotective pathways except photorespiration can protect the photosynthetic apparatus from oxidative damage in response to varying intensities of drought stress when CO2 assimilation is restricted. This is helpful for the gradual recovery of photosynthetic capacity after rewatering. Thus, F. rufa plants can withstand drought and is capable of survival in such environment. Highlights:1. The effects of drought and subsequent rewatering on Fargesia rufa were studied.2. Drought resulted in a reversible inhibition of photochemistry.3. Photoprotective pathways except photorespiration were up-regulated at the drought phase.4. Rewatering rapidly restored photochemical activity, especially under moderate drought.5. Fargesia rufa plant is capable of resisting and surviving drought environment.

Carbohydrate dynamics of three dominant species in a Chinese savanna under precipitation exclusion

The potential impact of drought on the carbon balance in plants has gained great attention. Non-structural carbohydrate (NSC) dynamics have been suggested as an important trait reflecting carbon balance under drought conditions. However, NSC dynamics under drought and the response mechanisms of NSC to drought remain unclear, especially in water-limited savanna ecosystems. A precipitation exclusion experiment was performed to simulate different drought intensities in a savanna ecosystem in Yuanjiang valley in southwestern China. Growth, total NSC concentration and diurnal change of NSC were determined for the leaves and non-photosynthetic organs of three dominant species (Lannea coromandelica, Polyalthia cerasoides and Heteropogon contortus) throughout the growing season. Drought significantly reduced the growth of all the three species. Total NSC concentration averaged ~8.1%, varying with species, organ and sampling period, and did not significantly decrease under drought stress. By contrast, the diurnal change of NSC in these three species increased under drought stress. These results indicate that these three dominant species did not undergo carbon limitation. Thus, relative change in NSC is a more sensitive and effective indicator than carbon reserves in evaluation of plant carbon balance. These findings provide new insights for the understanding of carbon balance and the mechanisms of carbon starvation.

Phosphorous fractions in soils of rubber-based agroforestry systems: Influence of season, management and stand age

Rubber-based agroforestry system is a vital management practice and its productivity is often controlled by soil phosphorus (P) nutrient, but little information is available on P fractions dynamics in such system. The aim of this study was to examine the seasonal, management and stand age effects on P fractions, acid phosphatase activity, microbial biomass P, other physical-chemical properties and litter and roots in four systems: 10-year-old rubber mono- (YM) and intercropping (YI) with N-fixing species (NFS), 22-year-old mono- (MM) and intercropping (MI) in Xishuangbanna, Southwestern China. Most P fractions varied seasonally at different depths, with highest values in the fog-cool season (i.e. labile P at 5-60cm, non-labile P and total P at 30-60cm). By contrast, moderately labile P varied little over time, except in MI that had lower values in the rainy season. Compared with their monoculture counterparts, YI doubled resin-Pi concentration but decreased NaHCO3-extractable P, HCl-Pi and residual-Po at the 0-30cm depth, whereas MI had hardly any changes in P species at the 60-cm depth. Surprisingly, residual-Po was enriched down to the deepest soil (30-60cm) in both YI and MI in the fog-cool season. All P fractions, except NaOH0.1-Pi, were greatly reduced with increasing stand age. In addition to plants uptake, these changes can be explained by seasonality in soil environments (e.g. moisture, temperature, pH and microbial activity) and decomposition of litter and roots. Moreover, YI decreased labile Po stock, but MI increased moderately labile Pi at the 60-cm depth across seasons. The results imply that a large amount of residual-Po exists in acidic Oxisol from China and that they can be reasonably exploited to reduce the application of P fertilizers, highlighting the importance of Po pool. Taken together, intercropping mature rubber plantation with NFS appears to be an effective way to enhance productivity while maintaining adequate soil P concentration over the long run.

Mobile carbon supply in trees and shrubs at the alpine treeline ecotone

Although the growth limitation hypothesis (GLH) is the most accepted physiological explanation for alpine treeline formation, the debate about its formation mechanisms still remains controversial due to contradictory findings from different studies. The functional difference between trees and alpine low-stature shrubs may hold answers, as shrubs grow well at higher elevations. We investigated carbon (C) storage in deciduous treeline species Betula ermanii (Erman’s birch) and two dominant shrubs (deciduous Vaccinium uliginosum and evergreen Rhododendron aureum), which naturally grow next to each other at the treeline ecotone on Changbai Mountain, Northeast China. We determined growth and non-structural carbohydrate (NSC) concentrations in organs with increasing elevation at the mid-growing season. Results showed that in the treeline ecotone soil temperature was lower than tree canopy air temperature due to unobvious aerodynamic decoupling near the ground. Species growth consistently decreased with increasing elevation, while NSC concentrations responded differently to elevation between trees and shrubs. An elevational increase and decrease in NSC were observed in leaves and woody organs, respectively, of B. ermanii. NSC concentrations in each organ significantly increased with increasing elevation for R. aureum but decreased for V. uliginosum. At the treeline, shrubs had higher values than B. ermanii in NSC, ratios of soluble sugars to starch in leaves, and leaf mass per area. Organ dependence of NSC with increasing elevation in Betula trees provided partial support for the GLH, while R. aureum and V. uliginosum provided strong support for the GLH and carbon limitation hypothesis, respectively. These imply that alpine shrubs may have evolved to maintain more advantageous C balance and functional features than did trees as an adaptation to higher-elevation climates.