Chenggui Zhang

Utilization of Polislidae Wasp Venom as Potential New Insect Drugs in the R&D of Wellness Industry

The Polislidae wasp, one species of omnivorous social insects mainly living in the bush or under the leaves. The wasp has a venom sac in its tail, and the venom secreted by a sting can cause a series of body reactions and diseases. Multiple organ failure could be the outcome of wasp sting, if timely treatment or rescue has not been performed. Based on published reports on wasp sting related to medical concerns in recent years, this review summarizes the symptoms caused by wasp sting and corresponding mechanisms of actions. The medical application and relational utilization of the title insect is suggested derived from findings of the systematic review. Furthermore, we herewith sketch the perspectives of R&D on the venom of Polislidae wasp. It is expected to afford comprehensive references and be useful for broader study on the natural components and pharmacological effects of wasp venom.

The protective role of all-transretinoic acid (ATRA) against colorectal cancer development is achieved via increasing miR-3666 expression and decreasing E2F7 expression

OBJECTIVE Colorectal cancer (CRC) is one of the most common malignancies with high morbidity and mortality rates worldwide. This study aimed to investigate whether miR-3666 was involved in inhibitory effects of all-transretinoic acid (ATRA) on the development of colorectal cancer (CRC). MATERIAL AND METHODS Surgical specimens of CRC tissues and adjacent non-tumor mucosa were collected for determining miR-3666 expression. Human CRC HCT116 cells were treated with different doses of ATRA (10, 20, 40, and 60 μM, respectively) and/or transfected with miR-3666 mimic, miR-3666 inhibitor, E2F7 siRNAs or their controls, respectively. After different treatments, cell viability, apoptosis, migration and invasion were detected. The regulatory relationship between miR-3666 and E2F7 was investigated. Furthermore, the association between MAPK/ERK pathway and ATRA or miR-3666/E2F7 was explored. RESULTS The miR-3666 was lowly expressed in CRC tissues, while E2F7 was highly expressed. ATRA decreased HCT116 cell viability, migration, and invasion, and induced apoptosis, indicating that ATRA inhibited the malignant behaviors of HCT116 cells. Moreover, ATRA increased miR-3666 expression, and effects of ATRA on the malignant behaviors of HCT116 cells were achieved by positive regulating miR-3666 expression. Furthermore, E2F7 was a target gene of miR-3666, and knockdown of E2F7 reversed the combined effects of ATRA and miR-3666 inhibitor on the malignant behaviors of HCT116 cells. Besides, ATRA inhibited the activation of MAPK/ERK signaling pathway, which was reversed by inhibition of miR-3666. CONCLUSIONS Our results reveal that ATRA protects against CRC development possible via increasing miR-3666 expression and decreasing E2F7 expression. MiR-3666/E2F7 may play a key role in regulating the inhibitory effects of ATRA on HCT116 cells via suppressing the activation of MAPK/ERK signaling pathway.

The History and Outlook of Animal Drugs Treating Asthma, Chronic Bronchitis, and Haze Episode-induced Respiratory Diseases

Animal drugs have been historically applied in Chinese remedies for more than two thousands. It was reported that Chinese medical animals consisting of 1,590 species took up 12.5% of the total number of all TCM resources. Those animal drugs such as, earthworm, gecko, periostracum cicadae , and scorpios, of commonly used in China, are very remarkable and traditional for the treatment of asthma or chronic bronchitis. This review presents research advance of animal drugs possessing significant implications for the development of novel anti-asthma or chronic bronchitis drugs. The experimental studies and clinical efficacies against asthma and chronic bronchitis of animal drugs were summarized herein. Moreover, the potential utilization of animal drugs on inhibiting haze/fog induced respiratory diseases was also discussed