Chi-Fang Chen

Neural mechanisms of impaired micturition reflex in rats with acute partial bladder outlet obstruction

To determine the contribution of neural elements to micturition, we evaluated, in intact rats, the cystometrogram, pelvic afferent nervous activity, pelvic efferent nervous activity and external urethral sphincter-electromyogram activity in the normal and acute partial bladder outlet obstruction states. In the normal state, in response to saline filling, mechanoreceptor-dependent pelvic afferent nervous activity gradually activated and finally triggered a voiding reflex, including four phases of detrusor contractions. Phase 1 was characterized by an initial rising intravesical pressure, Phase 2 was characterized by a series of high-frequency oscillations in intravesical pressure, Phase 3 contraction was characterized by a rebound intravesical pressure and Phase 4 contraction was characterized by a rapid fall in intravesical pressure. In the acute partial bladder outlet obstruction state, Phase 1 contraction rose and high-frequency oscillations fell in Phase 2. This voiding dysfunction is ascribed to the bursting extraurethral sphincter activity being converted to tonic extraurethral sphincter activity. In summary, the suppressed high-frequency oscillations in Phase 2 of the detrusor muscle contraction could be detrimental to efficient voidings in the acute partial bladder outlet obstructed rat.

Oxidative stress in bilateral total knee replacement, under ischaemic tourniquet

Free radicals, such as reactive oxygen species (ROS) which are released abruptly after deflation of an ischaemic tourniquet, cause reperfusion injuries. Ischaemic precondition (IPC), however, can reduce the injury. In clinical practice, the sequential application and release of tourniquets is often used in bilateral total knee replacement (TKR) to obtain a clearer operative field, but the effects on the production of free radicals and lipid peroxidation have not been studied. In this study, we have observed the production of free radicals and the subsequent lipid peroxidation in bilateral TKR with sequential application of a tourniquet to examine the effect of IPC. Patients undergoing elective TKR under intrathecal anaesthesia were studied. Blood samples were obtained after spinal anaesthesia, one minute before and five and 20 minutes after release of each tourniquet. We used the lucigenin chemiluminescence analysis and the phosphatidylcholine hydroperoxide (PCOOH) assay to measure the production of ROS and lipid peroxidation. Our results showed that production of ROS significantly increased at five and 20 minutes after release of the first tourniquet and at five minutes after release of the second tourniquet, but returned to normal at 20 minutes after the second reperfusion. The peak production of ROS was at 20 minutes after the first reperfusion; lipid peroxidation did not change significantly. We conclude that in spite of significant production of ROS after the release of tourniquet, the IPC phenomenon occurs during bilateral TKR with sequential application of a tourniquet.

Coupled Ocean–Acoustic Prediction of Transmission Loss in a Continental Shelfbreak Region: Predictive Skill, Uncertainty Quantification, and Dynamical Sensitivities

In this paper, we quantify the dynamical causes and uncertainties of striking differences in acoustic transmission data collected on the shelf and shelfbreak in the northeastern Taiwan region within the context of the 2008 Quantifying, Predicting, and Exploiting Uncertainty (QPE 2008) pilot experiment. To do so, we employ our coupled oceanographic (4-D) and acoustic (Nx2-D) modeling systems with ocean data assimilation and a best-fit depth-dependent geoacoustic model. Predictions are compared to the measured acoustic data, showing skill. Using an ensemble approach, we study the sensitivity of our results to uncertainties in several factors, including geoacoustic parameters, bottom layer thickness, bathymetry, and ocean conditions. We find that the lack of signal received on the shelfbreak is due to a 20-dB increase in transmission loss (TL) caused by bottom trapping of sound energy during up-slope transmissions over the complex and deeper bathymetry. Sensitivity studies on sediment properties show larger but isotropic TL variations on the shelf and smaller but more anisotropic TL variations over the shelfbreak. Sediment sound-speed uncertainties affect the shape of the probability density functions of the TLs more than uncertainties in sediment densities and attenuations. Diverse thicknesses of sediments lead to only limited effects on the TL. The small bathymetric data uncertainty is modeled and also leads to small TL variations. We discover that the initial transport conditions in the Taiwan Strait can affect acoustic transmissions downstream more than 100 km away, especially above the shelfbreak. Simulations also reveal internal tides and we quantify their spatial and temporal effects on the ocean and acoustic fields. One type of predicted waves are semidiurnal shelfbreak internal tides propagating up-slope with wavelengths around 40-80 km, horizontal phase speeds of 0.5-1 m/s, and vertical peak-to-peak displacements of isotherms of 20-60 m. These waves lead to variations of broadband TL estimates over 5-6-km range that are more isotropic and on bearing average larger (up to 5-8-dB amplitudes) on the shelf than on the complex shelfbreak where the TL varies rapidly with bearing angles.

A THREE-DIMENSIONAL AZIMUTHAL WIDE-ANGLE MODEL FOR THE PARABOLIC WAVE EQUATION

In predicting wave propagations in either direction, the size of the angle of propagation plays an important role; thus, the concept of wide-angle is introduced. Most existing acoustic propagation prediction models do have the capability of treating the wide-angle but the treatment, in practice, is vertical. This is desirable for solving two-dimensional (range and depth) problems. In extending the two-dimensional treatment to 3 dimensions, even though the wide-angle capability is maintained in most 3D models, it is still vertical. Owing to the need of a wide-angle capability in the azimuth direction, this paper formulates an azimuthal wide-angle wave equation whose theoretical development is presented. An illustrative example is included to demonstrate the need for such azimuthal wide-angle capability. Also, a comparison is shown between results using narrow-angle and wide-angle equations separately.

EXPERIMENTAL EVIDENCE OF HORIZONTAL REFRACTION BY NONLINEAR INTERNAL WAVES OF ELEVATION IN SHALLOW WATER IN THE SOUTH CHINA SEA: 3D VERSUS Nx2D ACOUSTIC PROPAGATION MODELING

A joint Taiwanese-U.S. field experiment was conducted in the South China Sea (SCS), entitled the South China Sea Oceanic Processes Experiment (Taiwan)/Non-Linear Internal Waves Initiative (US) (SCOPE/NLIWI), the ocean acoustics portion of which occurred during April 12–22, 2007. The acoustics objective was to quantify the temporal and spatial variability in acoustic propagation characteristics on the continental shelf in the presence of locally-generated and trans-basin nonlinear internal waves (NLIW). Broadband (400 Hz center frequency) m-sequence signals transmitted nearly continuously by a source moored near the seabed were received by vertical line arrays at 3 and 6 km range. The acoustic transect was oriented approximately parallel to the wave fronts of the shoaling trans-basin NLIWs which had crossed the deep basin from their origin in the Luzon Strait. The acoustic propagation variability due to strong vertical and horizontal refraction induced by the very large NLIWs creates an extremely complex acoustic field as a function of time and space. Experimental data and numerical acoustic propagation modeling results are presented to (1) examine and estimate the contribution of internal wave induced horizontal refraction to the received acoustic field; and (2) to quantify the range of propagation angles relative to the internal wave fronts within which strong horizontal refraction occurs and 3D propagation models are required to accurately predict the range- and depth-dependent acoustic propagation.

ACOUSTIC THREE-DIMENSIONAL EFFECTS AROUND THE TAIWAN STRAIT: COMPUTATIONAL RESULTS

A set of experiments were performed in the offshore area off the coasts of Taiwan and three-dimensional (3-D) measurements recorded. The 3-D effect on underwater propagation due to azimuthal variation of bottom topography is studied for the offshore regions southwest of Taiwan, where submarine canyons exist. A 3-D acoustic propagation model, FOR3D, is used to detect the 3-D effect. Computational results show that the 3-D effect is more prominent along the axis of the canyon than across it. Calculations show a very good agreement with field data, which indicate that the 3-D effect exists in this realistic ocean environment.

Passive acoustic monitoring of the temporal variability of odontocete tonal sounds from a long-term marine observatory.

The developments of marine observatories and automatic sound detection algorithms have facilitated the long-term monitoring of multiple species of odontocetes. Although classification remains difficult, information on tonal sound in odontocetes (i.e., toothed whales, including dolphins and porpoises) can provide insights into the species composition and group behavior of these species. However, the approach to measure whistle contour parameters for detecting the variability of odontocete vocal behavior may be biased when the signal-to-noise ratio is low. Thus, methods for analyzing the whistle usage of an entire group are necessary. In this study, a local-max detector was used to detect burst pulses and representative frequencies of whistles within 4.5–48 kHz. Whistle contours were extracted and classified using an unsupervised method. Whistle characteristics and usage pattern were quantified based on the distribution of representative frequencies and the composition of whistle repertoires. Based on the one year recordings collected from the Marine Cable Hosted Observatory off northeastern Taiwan, odontocete burst pulses and whistles were primarily detected during the nighttime, especially after sunset. Whistle usage during the nighttime was more complex, and whistles with higher frequency were mainly detected during summer and fall. According to the multivariate analysis, the diurnal variation of whistle usage was primarily related to the change of mode frequency, diversity of representative frequency, and sequence complexity. The seasonal variation of whistle usage involved the previous three parameters, in addition to the diversity of whistle clusters. Our results indicated that the species and behavioral composition of the local odontocete community may vary among seasonal and diurnal cycles. The current monitoring platform facilitates the evaluation of whistle usage based on group behavior and provides feature vectors for species and behavioral classification in future studies.

Enhanced acoustic mode coupling resulting from an internal solitary wave approaching the shelfbreak in the South China Sea

Internal waves and bathymetric variation create time- and space-dependent alterations in the ocean acoustic waveguide, and cause subsequent coupling of acoustic energy between propagating normal modes. In this paper, the criterion for adiabatic invariance is extended to the case of an internal solitary wave (ISW) encountering a sloping bathymetry (i.e., continental shelfbreak). Predictions based on the extended criterion for adiabatic invariance are compared to experimental observations from the Asian Seas International Acoustics Experiment. Using a mode 1 starter field, results demonstrate time-dependent coupling of mode 1 energy to higher adjacent modes, followed by abrupt coupling of mode 5-7 energy to nonadjacent modes 8-20, produces enhanced mode coupling and higher received levels downrange of the oceanographic and bathymetric features. Numerical simulations demonstrate that increasing ISW amplitude and seafloor slope enhance the coupling of energy to adjacent and nonadjacent modes. This enhanced coupling is the direct result of the simultaneous influence of the ISW and its proximity to the shelfbreak, and, compared to the individual effect of the ISW or shelfbreak, has the capacity to scatter 2-4 times the amount of acoustic energy from below the thermocline into the upper water column beyond the shelfbreak in realistic environments.

Focused sound from three-dimensional sound propagation effects over a submarine canyon

Ship noise data reveal an intensification of the near-surface sound field over a submarine canyon. Numerical modeling of sound propagation is used to study the effect. The noise data were collected during an ocean acoustic and physical oceanography experiment northeast of Taiwan in 2009. In situ measurements of water sound-speed profiles and a database of high-resolution bathymetry are used in the modeling study. The model results suggest that the intensification is caused by three-dimensional sound focusing by the concave canyon seafloor. Uncertainties in the model results from unsampled aspects of the environment are discussed.

Acoustics and oceanographic observations collected during the QPE Experiment by Research Vessels OR1, OR2 and OR3 in the East China Sea in the Summer of 2009

Abstract : This document describes data, sensors, and other useful information pertaining to the ONR sponsored QPE field program to quantify, predict and exploit uncertainty in observations and prediction of sound propagation. This experiment was a joint operation between Taiwanese and U.S. researchers to measure and assess uncertainty of predictions of acoustic transmission loss and ambient noise, and to observe the physical oceanography and geology that are necessary to improve their predictability. This work was performed over the continental shelf and slope northeast of Taiwan at two sites: one that was a relatively flat, homogeneous shelf region and a more complex geological site just shoreward of the shelfbreak that was influenced by the proximity of the Kuroshio Current. Environmental moorings and ADCP moorings were deployed and a shipboard SeaSoar vehicle was used to measure environmental spatial structure. In addition, multiple bottom moored receivers and a horizontal hy drophone array were deployed to sample transmission loss from a mobile source and ambient noise. The acoustic sensors, environmental sensors, shipboard resources, and experiment design, and their data, are presented and described in this technical report.