Chi Shing Cheung

High resolution Fourier domain optical coherence tomography in the 2 μm wavelength range using a broadband supercontinuum source.

A 220 nm bandwidth supercontinuum source in the two-micron wavelength range has been developed for use in a Fourier domain optical coherence tomography (FDOCT) system. This long wavelength source serves to enhance probing depth in highly scattering material with low water content. We present results confirming improved penetration depth in high opacity paint samples while achieving the high axial resolution needed to resolve individual paint layers. This is the first FDOCT developed in the 2 μm wavelength regime that allows fast, efficient capturing of 3D image cubes at a high axial resolution of 13 μm in air (or 9 μm in paint).

Optical coherence tomography in the 2-μm wavelength regime for paint and other high opacity materials

An optical coherence tomography system using a compact fiber source emitting amplified spontaneous emission at central wavelength of 1960 nm with bandwidth of 40 nm is developed to enhance the probing depth in a highly scattering material with low water content. Examples of application to paint are used to demonstrate significantly improved penetration depth in high opacity materials in the 2-μm wavelength regime.

Wavelength-swept Tm-doped fiber laser operating in the two-micron wavelength band

A wavelength-swept thulium-doped silica fiber laser using an intracavity rotating slotted-disk wavelength scanning filter in combination with an intracavity solid etalon for passive control of temporal and spectral profiles is reported. The laser yielded a wavelength swept output in a step-wise fashion with each laser pulse separated from the previous pulse by a frequency interval equal to the free-spectral-range of the etalon and with an instantaneous linewidth of <0.05 nm. Scanning ranges from 1905 nm to 2049 nm for a cladding-pumping laser configuration, and from 1768 nm to 1956 nm for a core-pumping laser configuration were achieved at average output powers up to ~1 W.

The origins of the Selden map of China: scientific analysis of the painting materials and techniques using a holistic approach

Since the ‘rediscovery’ of the Selden map of China, an early seventeenth century map of Asia, in the Bodleian Library in Oxford, the importance of the map in our understanding of globalisation in the early seventeenth century has been recognised. One of the unresolved questions is the origin of the map. This paper addresses the question through material evidence provided by a holistic approach using a suite of complementary analytical techniques. The map was examined in situ and non-invasively by a remote spectral imaging instrument (PRISMS) modified for close range imaging, which was followed by a range of complementary techniques applied to a number of detached fragments, though most of the techniques are non-invasive and can be applied to the map directly in the future. The binding medium was found to be a gum, almost certainly gum Arabic, rather than the animal glue commonly used in Chinese paintings. Some of the pigments and their usage were found to be at odds with the common practice in paintings from China. The detection of gum Arabic, a binding medium used by the Europeans, South and West Asians and the use of a mixture of orpiment and indigo, commonly found in European, South and West Asian paintings gives further evidence on the unusual origins of this map. The likely detection of a basic copper chloride, such as atacamite, in the green areas suggests an influence from the South and West Asian rather than the European tradition. Detailed analysis of the various spectral bands of the spectral image cube along with visual inspection of the large scale colour image showed that the map was not fully planned at the beginning but rather painted in stages, at times by trial and error and that it was unfinished. A new hypothesis for the origin of the Selden map in Aceh Sumatra is proposed based on the new evidences.

Long wavelength optical coherence tomography for painted objects

Optical Coherence Tomography has been successfully applied to the imaging of painted objects in recent years. However, a significant limitation is the low penetration depth of OCT in paint because of the high opacity of paint due to either scattering or absorption. It has been shown that the optimum spectral window for OCT imaging of paint layers is around 2.2μm in wavelength. In this paper, we demonstrate a 1950nm OCT for imaging painted objects using a superfluorescent fiber source at low power.

Optical coherence tomography and non-linear microscopy for paintings – a study of the complementary capabilities and laser degradation effects

This paper examines for the first time the potential complementary imaging capabilities of Optical coherence tomography (OCT) and non-linear microscopy (NLM) for multi-modal 3D examination of paintings following the successful application of OCT to the in situ, non-invasive examination of varnish and paint stratigraphy of historic paintings and the promising initial studies of NLM of varnish samples. OCT provides image contrast through the optical scattering and absorption properties of materials, while NLM provides molecular information through multi-photon fluorescence and higher harmonics generation (second and third harmonic generation). OCT is well-established in the in situ non-invasive imaging of the stratigraphy of varnish and paint layers. While NLM examination of transparent samples such as fresh varnish and some transparent paints showed promising results, the ultimate use of NLM on paintings is limited owing to the laser degradation effects caused by the high peak intensity of the laser source necessary for the generation of non-linear phenomena. The high intensity normally employed in NLM is found to be damaging to all non-transparent painting materials from slightly scattering degraded varnish to slightly absorbing paint at the wavelength of the laser excitation source. The results of this paper are potentially applicable to a wide range of materials given the diversity of the materials encountered in paintings (e.g. minerals, plants, insects, oil, egg, synthetic and natural varnish).

Rapid in situ imaging and whole genome sequencing of biofilm in neonatal feeding tubes: A clinical proof of concept

The bacterial flora of nasogastric feeding tubes and faecal samples were analysed for a low-birth weight (725 g) neonate EGA 25 weeks in intensive care. Samples were collected at age 6 and 8 weeks of life. Optical coherence tomography (OCT) was used to visualise bacterial biofilms inside the nasogastric feeding tubes. The biofilm was heterogeneously distributed along the tube lumen wall, and had a depth of up to 500 µm. The bacterial biofilm and faecal samples included Enterococcus faecalis and Enterobacter hormaechei. Representative strains, recovered from both feeding tubes and faecal samples, were whole genome sequenced using Illumina, Mi-Seq, which revealed indistinguishable strains, each with less than 28 SNP differences, of E. faecalis and E. hormaechei. The E. faecalis strains were from two sequence types (ST191 and ST211) and encoded for a number of traits related to biofilm formation (BopD), adherence (Epb pili), virulence (cps loci, gelatinase, SprE) and antibiotic resistances (IsaA, tetM). The E. hormaechei were all ST106, and encoded for blaACT-15 β–lactamase and fosfomycin resistance (fosA). This proof of concept study demonstrates that bacterial flora within the neonatal feeding tubes may influence the bacterial colonisation of the intestinal tract and can be visualised non-destructively using OCT.

Ultra-high resolution Fourier domain optical coherence tomography for resolving thin layers in painted works of art

While OCT has been applied to the non-invasive examination of the stratigraphy of paint layers in recent years, it has been recognized that the resolutions of commercially available OCT cannot compete in depth resolution with conventional microscopic examination of cross-sections of paint samples. It is necessary to achieve resolutions better than 3 microns to resolve the thinnest layers of paint and varnish. In this paper, we demonstrate a Fourier domain ultrahigh resolution OCT at 810nm with depth resolution of 1.8 μm in air (or 1.2μm in varnish or paint).

High resolution Fourier domain Optical Coherence Tomography at 2 microns for painted objects

Optical Coherence Tomography has been successfully applied to the non-invasive imaging of subsurface microstructure of a variety of materials from biological tissues to painted objects of art. One of the limitations of the technique is the low depth of penetration due to the strong scattering and absorption in the material. Previous studies found that for paint materials, the optimum window for large depth of penetration is around 2.2 microns. This is also true for many other materials with low water content. We have previously demonstrated OCT systems in this wavelength regime for imaging with improved depth of penetration. In this paper, we present an improved 2 micron high resolution Fourier domain OCT system using a broadband supercontinuum source. The system achieved a depth resolution of 9 microns in air (or 6 microns in paint or any polymer).