Chiung Yao Huang
National Sun Yat-sen University
Network
Latest external collaboration on country level. Dive into details by clicking on the dots.
Publication
Featured researches published by Chiung Yao Huang.
Journal of Natural Products | 2010
Bo Wei Chen; Shu Ming Chang; Chiung Yao Huang; Chih Hua Chao; Jui Hsin Su; Zhi Hong Wen; Chi Hsin Hsu; Chang-Feng Dai; Yang Chang Wu; Jyh-Horng Sheu
Eight new eunicellin-base diterpenoids, hirsutalins A-H (1-8), were isolated from the soft coral Cladiella hirsuta. Their structures were elucidated by spectroscopic methods, particularly in 1D and 2D NMR experiments. The absolute configuration of 1 was determined by Moshers method. Compounds 1, 5, and 6 have been shown to exhibit cytotoxicity toward several cancer cell lines. Compounds 2-4 and 8 were found to display significant in vitro anti-inflammatory activity in LPS-stimulated RAW264.7 macrophage cells by inhibiting the expression of the iNOS protein, with compound 2 also effectively reducing the level of COX-2 protein.
Organic and Biomolecular Chemistry | 2011
Bo Wei Chen; Chih Hua Chao; Jui Hsin Su; Chung Wei Tsai; Wei Hsien Wang; Zhi Hong Wen; Chiung Yao Huang; Ping-Jyun Sung; Yang Chang Wu; Jyh-Horng Sheu
New eunicellin-base diterpenoids, klysimplexins I-T (1-12), were isolated from a cultured soft coral Klyxum simplex. Their structures were elucidated by spectroscopic methods, particularly in 1D and 2D NMR experiments. The absolute stereochemistry of 4 was determined by Moshers method. Compounds 9 and 12 have been shown to exhibit cytotoxicity toward a limited panel of cancer cell lines. Compounds 2-6, 10 and 11 were found to display significant in vitro anti-inflammatory activity in LPS-stimulated RAW264.7 macrophage cells by inhibiting the expression of the iNOS protein. Compounds 10 and 11 also could effectively reduce the level of COX-2 protein.
Marine Drugs | 2011
Chih Hua Chao; Kuei Ju Chou; Chiung Yao Huang; Zhi Hong Wen; Chi Hsin Hsu; Yang Chang Wu; Chang-Feng Dai; Jyh-Horng Sheu
Eight new cembranoids, crassarines A–H (1–8) were isolated from the Formosan soft coral Sinularia crassa. Compounds 1–3 represent the rare cembranoids with a 1,12-oxa-bridged tetrahydrofuran ring, while 4 and 5 are the firstly discovered 1,11-oxa-bridged tetrahydropyranocembranoids. The absolute configuration of 6 was determined using the Mosher’s method. Compounds 6 and 8 were found to significantly inhibit the expression of both pro-inflammatory iNOS and COX-2 proteins at 10 μM, respectively, while compounds 4–8 were found to be non-cytotoxic toward the selected human liver cancer cells.
Organic and Biomolecular Chemistry | 2011
Bo Wei Chen; Shu Ming Chang; Chiung Yao Huang; Jui Hsin Su; Zhi Hong Wen; Yang Chang Wu; Jyh-Horng Sheu
Seven new polyoxygenated steroids, hirsutosterols A-G (1-7), were isolated from the Formosan soft coral Cladiella hirsuta. Their structures were elucidated by spectroscopic methods, particularly in 1D and 2D NMR experiments. The absolute configurations of 1 and 5 were determined by Moshers method. Sterols 4-6 possess hydroxy groups at C-9 and C-11 and might be oxidatively cleaved to the corresponding 9,11-secosterols. Hirsutosterol A (1) was found to exhibit a stronger cytotoxicity against a limited panel of cancer cell lines.
Journal of Natural Products | 2011
Fang Jung Hsu; Bo Wei Chen; Zhi Hong Wen; Chiung Yao Huang; Chang-Feng Dai; Jui Hsin Su; Yang Chang Wu; Jyh-Horng Sheu
Eight new eunicellin-based diterpenoids, klymollins A-H (1-8), were isolated during the chemical investigation of the soft coral Klyxum molle from Taiwan waters. Their structures were elucidated by extensive spectroscopic analysis. The absolute configuration of 4 was determined by Moshers method. Bioassays of the new metabolites showed that compounds 3-8 displayed significant in vitro anti-inflammatory activity by inhibiting the expression of the iNOS protein, and compounds 3, 4, and 6-8 also could effectively reduce the accumulation of COX-2 protein in LPS-stimulated RAW264.7 macrophage cells.
Marine Drugs | 2012
Chih Hua Chao; Kuei Ju Chou; Chiung Yao Huang; Zhi Hong Wen; Chi Hsin Hsu; Yang Chang Wu; Chang-Feng Dai; Jyh-Horng Sheu
One new sterol, crassarosterol A (1), and four new steroidal glycosides, crassarosterosides A–D (2–5) were isolated from the Formosan soft coral Sinularia crassa. The absolute configuration of 1 was determined using the Mosher’s method. The absolute configurations for the sugar moieties of 2–5 were determined by HPLC analysis on the o-tolylthiocarbamates derived from the liberated sugar after acid hydrolysis. Compounds 2 and 4 could significantly inhibit the expression of pro-inflammatory iNOS protein at 10 µM. In contrast, 1–3 were found to stimulate the expression of COX-2 protein at this concentration. Steroids 1 and 4 also showed cytotoxicity toward the selected human liver cancer cells.
Molecules | 2013
Wen Li Hsu; Siou Jin Chiu; Yao Ting Tsai; Che Mai Chang; Jaw Yan Wang; Eric Terry Wang; Ming Feng Hou; Chiung Yao Huang; Jyh-Horng Sheu; Wei Chiao Chang
Epidermal growth factor receptor (EGFR) is overexpressed in many types of cancer cells. EGFR-mediated signaling involves inflammatory gene expression including cyclooxygenase (COX)-2 and interleukin (IL)-8, and is associated with cancer pathogenesis. In a search of phytochemicals with anti-inflammatory activity, the COX-2 and IL-8 inhibitory activities of some marine compounds were examined. After screening these compounds 11-episinulariolide acetate (1) from soft coral exhibited the most potent activity. Reverse-transcription PCR; western blotting; ELISA and luciferase assays were used to test the effect of compound 1 on EGF-stimulated expressions of COX-2 and IL-8 in A431 human epidermoid carcinoma cells. After exposure to 10 μM of compound 1, expression levels of COX-2 and IL-8 were reduced. In addition; intracellular Ca2+ increase and Ca2+-dependent transcription factor activation were blocked by compound 1. Thus, compound 1 can potentially serve as a lead compound for targeting Ca2+ signaling-dependent inflammatory diseases.
Marine Drugs | 2017
Chih Hua Chao; Wen Liang Li; Chiung Yao Huang; Atallah F. Ahmed; Chang-Feng Dai; Yang Chang Wu; Mei Chin Lu; Chih-Chuang Liaw; Jyh-Horng Sheu
Five new isoprenoids, 3,4,8,16-tetra-epi-lobocrasol (1), 1,15β-epoxy-deoxysarcophine (2), 3,4-dihydro-4α,7β,8α-trihydroxy-Δ2-sarcophine (3), ent-sarcophyolide E (4), and 16-deacetyl- halicrasterol B (5) and ten known compounds 6‒15, were characterized from the marine soft coral Sarcophyton glaucum, collected off Taitung coastline. Their structures were defined by analyzing spectra data, especially 2D NMR and electronic circular dichroism (ECD). The structure of the known compound lobocrasol (7) was revised. Cytotoxicity potential of the isolated compounds was reported, too.
Marine Drugs | 2016
Chih Hua Chao; Chia Yun Wu; Chiung Yao Huang; Hui Chun Wang; Chang-Feng Dai; Yang Chang Wu; Jyh-Horng Sheu
Two new cubitanoids, nanoculones A and B (1 and 2), and three new cembranoids, nanolobols A–C (3–5), as well as six known compounds, calyculone I (6), sinulariuol A (7), sinulariols C, D, H, and J (8–11), were isolated from the soft coral Sinularia nanolobata, collected off the coast of the eastern region of Taiwan. Their structures were elucidated on the basis of extensive spectroscopic analysis. Cytotoxicity of compounds 1–11 was evaluated. The nitric oxide (NO) inhibitory activity of selected compounds was further measured by assay of lipopolysaccharide (LPS)-stimulated NO production in activated RAW264.7 cells. The results showed that none of 1–11 exhibited cytotoxicity against the tested cancer cell lines, whereas compound 8 was found to significantly reduce NO production.
Tetrahedron | 2013
Bo Wei Chen; Sheng-Yang Wang; Chiung Yao Huang; Shu Li Chen; Yang Chang Wu; Jyh-Horng Sheu