Choh Hao Li

Increase in Glyceride Content of Brown Fat by Treatment with Adrenocorticotropin.

Summary Treatment of intact or castrated rats with ACTH caused an increase in the glyceride content of brown adipose tissue. Treatment of hypophysectomized rats with 3 times the dose used above failed to maintain the fat content of the interscapular gland at a normal level.

CHARACTERIZATION OF 3H-β-ENDORPHIN BINDING IN RAT BRAIN

Summary 3 H-β-Endorphin (βEP) binding was characterized in rat brain homogenates. It exhibits many characteristics of opiate binding, including stereospecificity, inhibition by sodium, and displacement by all opiate agonists and antagonists tested. Interesting differences from 3 H-naloxone binding include some discrepancies in the profile of regional distribution and a significantly slower rate of dissociation of βP. Enkephalin and βEP exhibit a 25-fold difference in relative potency depending on which 3 H-Ligand they are competing against. These results may prove useful in exploring differences between subtypes of opiate receptors.