Christian W. Bauer

Refolding of the integral membrane protein light-harvesting complex II monitored by pulse EPR

The major light-harvesting chlorophyll a/b complex (LHCII) of the photosynthetic apparatus in plants self-organizes in vitro. The recombinant apoprotein, denatured in dodecyl sulfate, spontaneously folds when it is mixed with its pigments, chlorophylls, and carotenoids in detergent solution, and assembles into structurally authentic LHCII in the course of several minutes. Pulse EPR techniques, specifically double-electron-electron resonance (DEER), have been used to analyze protein folding during this process. Pairs of nitroxide labels were introduced site-specifically into recombinant LHCII and shown not to affect the stability and function of the pigment-protein complex. Interspin distance distributions between two spin pairs were measured at various time points, one pair located on either end of the second transmembrane helix (helix 3), the other one located near the luminal ends of the intertwined transmembrane helices 1 and 4. In the dodecyl sulfate-solubilized apoprotein, both distance distributions were consistent with a random-coil protein structure. A rapid freeze-quench experiment on the latter spin pair indicated that 1 s after initiating reconstitution the protein structure is virtually unchanged. Subsequently, both distance distributions monitored protein folding in the same time range in which the assembly of chlorophylls into the complex had been observed. The positioning of the spin pair spanning the hydrophobic core of LHCII clearly preceded the juxtaposition of the spin pair on the luminal side of the complex. This indicates that superhelix formation of helices 1 and 4 is a late step in LHCII assembly.

B decay shape variables and the precision determination of |V(cb)| and m(b)

We present expressions for shape variables of B decay distributions in several different mass schemes, to order

Power suppressed operators and gauge invariance in soft-collinear effective theory

\alpha_s^2\beta_0

Lasing in organic circular grating structures

and (Lambda_{QCD}/mb)^3. Such observables are sensitive to the b quark mass and matrix elements in the heavy quark effective theory, and recent measurements allow precision determinations of some of these parameters. We perform a combined fit to recent experimental results from CLEO, BABAR, and DELPHI, and discuss the theoretical uncertainties due to nonperturbative and perturbative effects. We discuss the possible discrepancy between the OPE prediction, recent BABAR results and the measured branching fraction to D and D* states. We find |Vcb| = (40.8 +- 0.9) x 10^{-3} and mb^{1S} = 4.74 +- 0.10 GeV, where the errors are dominated by experimental uncertainties.

Light-cone distribution functions for B decays at subleading order in 1=mb

The form of collinear gauge invariance for power suppressed operators in the soft-collinear effective theory is discussed. Using a field redefinition we show that it is possible to make any power suppressed ultrasoft-collinear operators invariant under the original leading order gauge transformations. Our manipulations avoid gauge fixing. The Lagrangians to O(lambda^2) are given in terms of these new fields. We then give a simple procedure for constructing power suppressed soft-collinear operators in SCET_II by using an intermediate theory SCET_I.

Design of a permanent magnet with a mechanical sweep suitable for variable-temperature continuous-wave and pulsed EPR spectroscopy

Optically pumped organic polymer lasers are fabricated by spin coating a thin polymer film onto a nanopatterned SiO2 circular-grating surface-emitting distributed Bragg reflector. For certain grating parameters, we observe a peak inside the stop band that leads to lasing with a reduced threshold. An analytical model, based on the transfer-matrix method, has been developed to investigate the origin of this peak. The theoretical results are in good agreement with the experimental findings.

Improving jet distributions with effective field theory.

We construct the higher twist structure functions that describe inclusive b hadron decays in regions of phase space where the hadronic decay products carry high energy but have low invariant mass. We show that, for B meson decays, there are four new non-vanishing matrix elements of non-local operators. We show that to subleading twist these decays are parametrized in terms of four functions. We compute the tree-level matching for a general heavy-to-light current and apply it to B ! Xs. Using a simple model for these functions we estimate the subleading twist contributions to this decay.

Factorization of boosted multijet processes for threshold resummation

A magnetic system is introduced which consists of three nested rings of permanent magnets of a Halbach dipolar layout and is capable for EPR spectroscopy. Two of the rings can be rotated independently to adjust the magnetic flux in the center and even allow for mechanical field sweeps. The presented prototype achieves a magnetic flux range of 0.0282-0.3013T with a minimal sweep of 0.15mT and homogeneity of about 10(-3). First applications with CW and pulsed Mims ENDOR as well as ESEEM experiments on a sample of a glycine single crystal doped with 1% copper nitrate demonstrate that flux range, sweep accuracy and homogeneity of this prototype is sufficient for EPR experiments on most solid samples. Together with a recently improved design magnets can be build which could serve as compact and easily transportable replacement of standard electromagnets with negligible consumption of power or coolants.

SCET Analysis of B -> K pi, B -> K Kbar, and B -> pi pi Decays

We obtain perturbative expressions for jet distributions using soft-collinear effective theory (SCET). By matching SCET onto QCD at high energy, tree level matrix elements and higher order virtual corrections can be reproduced in SCET. The resulting operators are then evolved to lower scales, with additional operators being populated by required threshold matchings in the effective theory. We show that the renormalization group evolution and threshold matchings reproduce the Sudakov factors and splitting functions of QCD, and that the effective theory naturally combines QCD matrix elements and parton showers. The effective theory calculation is systematically improvable and any higher order perturbative effects can be included by a well-defined procedure.

Shape function effects in B -> X_s gamma and B -> X_u l nu decays

Explicit applications of factorization theorems for processes at hadron colliders near the hadronic end point have largely focused on simple final states with either no jets (e.g., Drell-Yan) or one inclusive jet (e.g., deep-inelastic scattering and prompt photon production). Factorization for the former type of process gives rise to a soft function that depends on time like momenta, whereas the soft function for the latter type depends on null momenta. We derive in soft-collinear effective theory a factorization theorem that allows for an arbitrary number of jets, where the jets are defined with respect to a jet algorithm, together with any number of nonstrongly interacting particles. We find the soft function in general depends on the null components of the soft momenta inside the jets and on a timelike component of the soft momentum outside of the jets. This generalizes and interpolates between the soft functions for the cases of no jets and one inclusive jet. We verify consistency of our factorization theorem to O(α s ) for any number of jets. While in this paper we demonstrate consistency only near the hadronic end point, we keep the kinematics general enough (in particular allowing for nonzero boost) to allow for an extension to partonic threshold resummation away from the hadronic end point.