Christiane Kan

Production of indole alkaloids by in vitro root cultures from Catharanthus trichophyllus

Abstract Following in vitro infection of an aseptic Catharanthus trichophyllus plant with Agrobacterium rhizogenes (15834), six hairy root cultures were established. These were compared with normal root cultures, derived from the same plant, with respect to production of indole alkaloids. A rapidly growing hairy root line was used for fermenter (201) cultures. The alkaloid content of the roots obtained was examined. Seventeen monomeric indole alkaloids were purified and characterized, including five hitherto undescribed substances. Normal root and hairy root line cultures showed similar alkaloid composition. Analyses, performed at five-week intervals on five-week-old cultures showed variable alkaloid yields.

Epiallo-yohimbine derivatives isolated from in vitro hairy-root cultures of catharanthus trichophyllus

Abstract From the in vitro hairy-root cultures of Catharanthus trichophyllus have been isolated five new indole alkaloids, belonging to a new series of yohimbine type bases. Anthraserpine and dimethoxyanthraserpine have been found to contain the 11-methoxy- epiallo -yohimbine skeleton, esterified at C-18 with 2-acetamidobenzoic acid and 2-acetamido-4,5-dimethoxybenzoic acid, respectively. Their structures have been established by detailed spectral analysis. Isolated in trace amounts, three other congeners of the same series have been identified on the basis of their mass spectral data.

Determination de structures par RMN du 1H A 400 MH : Alcaloides de tabernaemontana albiflora.

Abstract The structures of two new alkaloids of the 21-nor (+) pandolane type from the bark of Tabernaemontana albiflora (Miq.) Pull. (Apocynaceae), were determined.

Indole Alkaloids from Kopsia deverrei

Abstract Three new monoterpene indole alkaloids, 16-hydroxymethyl-pleiocarpamine 2, 16-epi-deacetylakuammiline 4 and 14α-hydroxycondylocarpine 8, have been isolated from the stem bark of Kopsia deverrei (Apocynaceae). The proposed structures were assigned after detailed analysis of their 1H and 13C NMR spectra and by chemical correlation.

Determination de structures par RMN du 1H A 400 MHz: Quatre nouveaux alcaloides de Tabernaemontana albiflora

Resume The structures of four new alkaloids 1 – 4 of the 21-nor (+) pandolane type from the stem bark of Tabernaemontana albiflora (Miq.) Pull. (Apocynaceae) have been determined.

Alkaloids from Nitraria Billardieri

Abstract Five alkaloids have been isolated and identified in Nitraria billardieri from Australia. They consist of schoberine 1, nitraramine 2 1-epinitraramine 3, nitrarine 4 and 3-epinitrarine 5. Complete assignment of their NMR spectra is presented.

Structure of Aristoaristone, A New Dimeric Indole Alkaloid from Aristotelia Australasica

Abstract A new dimer, aristoaristone 3, has been isolated from Aristotelia australasica. It is comprised of one moiety each of aristone and aristolasicone linked by the dihydroindole nitrogen and an aromatic carbon. A tentative hemisynthesis from the two monomers led to oxidized products which were identified as bis-aristone B and 19-oxo-aristotelone 4.

Determination de structures par RMN du 1H A 400 MH : Alcaloides de .

Abstract The structures of two new alkaloids of the 21-nor (+) pandolane type from the bark of Tabernaemontana albiflora (Miq.) Pull. (Apocynaceae), were determined.

Determination de structures par RMN du 1H A 400 MHz: Quatre nouveaux alcaloides de

Resume The structures of four new alkaloids 1 – 4 of the 21-nor (+) pandolane type from the stem bark of Tabernaemontana albiflora (Miq.) Pull. (Apocynaceae) have been determined.

Structure determination using 1H nuclear magnetic resonance at 400 mhz: four new alkaloids from Tabernaemontana albiflora.

Resume The structures of four new alkaloids 1 – 4 of the 21-nor (+) pandolane type from the stem bark of Tabernaemontana albiflora (Miq.) Pull. (Apocynaceae) have been determined.