Christine Froidevaux

Towards a semi-automatic functional annotation tool based on decision-tree techniques

BackgroundDue to the continuous improvements of high throughput technologies and experimental procedures, the number of sequenced genomes is increasing exponentially. Ultimately, the task of annotating these data relies on the expertise of biologists. The necessity for annotation to be supervised by human experts is the rate limiting step of the data analysis. To face the deluge of new genomic data, the need for automating, as much as possible, the annotation process becomes critical.ResultsWe consider annotation of a protein with terms of the functional hierarchy that has been used to annotate Bacillus subtilis and propose a set of rules that predict classes in terms of elements of the functional hierarchy, i.e., a class is a node or a leaf of the hierarchy tree. The rules are obtained through two decision-trees techniques: first-order decision-trees and multilabel attribute-value decision-trees, by using as training data the proteins from two lactic bacteria: Lactobacillus sakei and Lactobacillus bulgaricus. We tested the two methods, first independently, then in a combined approach, and evaluated the obtained results using hierarchical evaluation measures. Results obtained for the two approaches on both genomes are comparable and show a good precision together with a high prediction rate. Using combined approaches increases the recall and the prediction rate.ConclusionThe combination of the two approaches is very encouraging and we will further refine these combinations in order to get rules even more useful for the annotators. This first study is a crucial step towards designing a semi-automatic functional annotation tool.

Extracting sequential nuggets of knowledge

We present the notion of sequential association rule and introduce Sequential Nuggets of Knowledge as sequential association rules with possible low support and good quality, which may be highly relevant to scientific knowledge discovery. Then we propose the algorithm SNK that mines some interesting subset of sequential nuggets of knowledge and apply it to an example of molecular biology. Unexpected nuggets that are produced may help scientists refine a rough preliminary classification. A first implementation in Java is freely available on the web.