Christine Stumpp

Biodegradation: Updating the Concepts of Control for Microbial Cleanup in Contaminated Aquifers

Biodegradation is one of the most favored and sustainable means of removing organic pollutants from contaminated aquifers but the major steering factors are still surprisingly poorly understood. Growing evidence questions some of the established concepts for control of biodegradation. Here, we critically discuss classical concepts such as the thermodynamic redox zonation, or the use of steady state transport scenarios for assessing biodegradation rates. Furthermore, we discuss if the absence of specific degrader populations can explain poor biodegradation. We propose updated perspectives on the controls of biodegradation in contaminant plumes. These include the plume fringe concept, transport limitations, and transient conditions as currently underestimated processes affecting biodegradation.

Water droplets in oil are microhabitats for microbial life

Microbial life thrives in an oily bubble Microorganisms can break down hydrocarbons in oil reservoirs. Microbes grow primarily at the interface between oil and water, where they can find nutrients and dispose of metabolites. Meckenstock et al. now show that tiny water droplets can also provide a suitable home for hydrocarbon-degrading microorganisms. The authors examined oil from Pitch Lake, Trinidad and Tobago, and found that diverse microorganisms thrived in these tiny isolated microhabitats. Science, this issue p. 673 Methanogenic microorganisms living in water droplets may contribute to the degradation of oil reservoirs. Anaerobic microbial degradation of hydrocarbons, typically occurring at the oil-water transition zone, influences the quality of oil reservoirs. In Pitch Lake, Trinidad and Tobago—the world’s largest asphalt lake—we found that microorganisms are metabolically active in minuscule water droplets (1 to 3 microliters) entrapped in oil. Pyrotag sequencing of individual droplet microbiomes revealed complex methanogenic microbial communities actively degrading the oil into a diverse range of metabolites, as shown by nuclear magnetic resonance and Fourier transform ion cyclotron resonance mass spectrometry. High salinity and water-stable isotopes of the droplets indicate a deep subsurface origin. The 13.5% water content and the large surface area of the droplets represent an underestimated potential for biodegradation of oil away from the oil-water transition zone.

Quantification of the heterogeneity of the unsaturated zone based on environmental deuterium observed in lysimeter experiments

Abstract An approach is presented which enables the quantification, in integrative form, of the preferential flow and the heterogeneity in water transport through the unsaturated zone under natural atmospheric conditions. For these purposes, deuterium transport was estimated in seven lysimeters filled with different sediment materials by applying a conceptual model, which consists of preferential and matrix flow paths for each using the lumped parameter approach. The piston flow and dispersion transit time distribution functions were assumed, for preferential and matrix flow paths, respectively. Weekly 2H data measured in precipitation (1984–1991) were taken as the input function. Combining modelling of isotopes and hydrological data enabled the quantification of preferential flow rates. The fraction of preferential flow directly appearing in the outflow within one week varied between 17 and 30%. The fraction of preferential flow was practically independent of the soil texture and flow rates. The crucial parameter influencing the fraction of direct flow was found to be the saturated hydraulic conductivity (Ks ). In the matrix flow, it was found that the apparent dispersion parameter (PD )* is indirectly proportional to the mean water content ( ). This relationship shows that the heterogeneity of the water flux in the matrix is higher for lower water contents. Finally, the transit time distribution functions, determined for both flow paths and the fractions of preferential flow, were used to construct vulnerability diagrams. Such a diagram gives the amounts of tracer mass (conservative pollutant), which appear weekly in the outflow between 0 and 60 weeks. The vulnerability diagrams showed different patterns for different soils, depending on the saturated conductivity and mean water content. Coarser material with low and high Ks showed a short mean transit time for the matrix flow (about 10 weeks) and mean preferential flow equal to or higher than 20%. Finer sand, with lower Ks and higher , resulted in mean transit times of approximately 30 weeks and preferential flow of 17–21%. These diagrams can be used to estimate the vulnerability of groundwater to pollution for different soil materials of the unsaturated zone.

Toward operational methods for the assessment of intrinsic groundwater vulnerability: A review

ABSTRACT Assessing the vulnerability of groundwater to adverse effects of human impacts is one of the most important problems in applied hydrogeology. At the same time, many of the widespread vulnerability assessment methods do not provide physically meaningful and operational indicators of vulnerability. Therefore, this review summarizes (i) different methods used for intrinsic vulnerability assessment and (ii) methods for different groundwater systems. It particularly focuses on (iii) timescale methods of water flow as an appropriate tool and (iv) provides a discussion on the challenges in applying these methods. The use of such physically meaningful indices based on timescales is indispensable for groundwater resources management.

Transport and Bacterial Interactions of Three Bacterial Strains in Saturated Column Experiments

The impact of bacteria-solid and bacteria-bacteria interactions on the transport of Klebsiella oxytoca, Burkholderia cepacia G4PR1, and Pseudomonas sp. #5 was investigated in saturated sand column experiments (L = 114 mm; ø = 33 mm) under constant water velocities (∼ 5 cm · h(-1)). Bacterial strains were injected into the columns as pulses either individually, simultaneously, or successively. A one-dimensional mathematical model for advective-dispersive transport and for irreversible and reversible bacterial kinetic sorption was used to analyze the bacterial breakthrough curves. Different sorption parameters were obtained for each strain in each of the three experimental setups. In the presence of other bacteria, sorption parameters for B. cepacia G4PR1 remained similar to results from individual experiments, indicating the presence of other bacteria generally had a lesser influence on its migration than for the other bacteria. K. oxytoca is more competitive for the sorption sites when simultaneously injected with the other bacteria. Ps. sp. #5 generally yielded the greatest detachment rates and the least affinity to attach to the sand, indicative of its mobility in groundwater systems. The results of this study clearly indicate both bacteria-solid and bacteria-bacteria interactions influence the migration of bacteria. A more complete understanding of such interactions is necessary to determine potential migration in groundwater systems.

Correcting Laser-Based Water Stable Isotope Readings Biased by Carrier Gas Changes

Recently, laser-based water stable isotope spectrometers have become popular as they enable previously impossible approaches of environmental observations. Consequently, they have been subjected to increasingly heterogeneous atmospheric conditions. However, there is still a severe lack of data on the impact of nonstandardized gas matrices on analyzer performances. Against this background, we investigated the influence of changing proportions of N2, O2, and CO2 in the carrier gas on the isotope measurements of a typical laser-based water stable isotope analyzer (Picarro L2120-i). We combined environmentally relevant mixtures of N2, O2, and CO2 with referenced, flash-evaporated water and found that isotope readings of the same water were altered by up to +14.57‰ for δ(18)O and -35.9‰ for δ(2)H. All tested relationships between carrier gas changes and respective isotope readings were strongly linearly correlated (R(2) > 0.99). Furthermore, an analyzer-measured variable allowed for reliable postcorrection of the biased isotope readings, which we additionally tested on field data. Our findings are of importance for environmental data obtained by analyzers based on the same technology. They are relevant for assays where inconsistent gas matrices or a mismatch in this regard between unknown and reference analyses cannot be excluded, which is in particular common when investigating the soil-vegetation-atmosphere continuum.

Response and recovery of a pristine groundwater ecosystem impacted by toluene contamination – A meso-scale indoor aquifer experiment

Microbial communities are the driving force behind the degradation of contaminants like aromatic hydrocarbons in groundwater ecosystems. However, little is known about the response of native microbial communities to contamination in pristine environments as well as their potential to recover from a contamination event. Here, we used an indoor aquifer mesocosm filled with sandy quaternary calciferous sediment that was continuously fed with pristine groundwater to study the response, resistance and resilience of microbial communities to toluene contamination over a period of almost two years, comprising 132days of toluene exposure followed by nearly 600days of recovery. We observed an unexpectedly high intrinsic potential for toluene degradation, starting within the first two weeks after the first exposure. The contamination led to a shift from oxic to anoxic, primarily nitrate-reducing conditions as well as marked cell growth inside the contaminant plume. Depth-resolved community fingerprinting revealed a low resistance of the native microbial community to the perturbation induced by the exposure to toluene. Distinct populations that were dominated by a small number of operational taxonomic units (OTUs) rapidly emerged inside the plume and at the plume fringes, partially replacing the original community. During the recovery period physico-chemical conditions were restored to the pristine state within about 35days, whereas the recovery of the biological parameters was much slower and the community composition inside the former plume area had not recovered to the original state by the end of the experiment. These results demonstrate the low resilience of sediment-associated groundwater microbial communities to organic pollution and underline that recovery of groundwater ecosystems cannot be assessed solely by physico-chemical parameters.

A decision tree tool supporting the assessment of groundwater vulnerability

The Water Framework Directive and Groundwater Directive aim at preserving and improving the groundwater status. Groundwater bodies are classified as being or not being at risk of failing to meet these objectives. Those at risk are subject to more precise risk assessment where the concept of vulnerability is considered in the pathway part of the source–pathway–receptor scheme. However, no further details on implementation strategies are provided. In order to support groundwater managers and decision-makers in implementation of programs protecting groundwater, a systematic operational approach based on a decision tree is proposed, which leads the user through the stages of vulnerability assessment. First, a problem has to be formulated related to a threatening of the quantitative and/or qualitative status of a groundwater body. Next, the stated problem needs to be related to the intrinsic or specific vulnerability. Methods used for the intrinsic vulnerability assessment belong to two categories: subjective rating and objective methods. Method selection depends primarily on: data availability, knowledge and available resources. A key issue is the lag time associated with transport between a source/event of contamination and the water body. This lag time is primarily controlled by the temporal scale of water flow. It provides information about flow processes and at the same time also about timescales required for the implementation of strategies. Effects of any measures taken cannot be observed immediately but at the earliest after these estimated lag times emphasizing the need to also proactively safeguard groundwater resources and preserve their good status.

Advantages and challenges of using soil water isotopes to assess groundwater recharge dominated by snowmelt at a field study located in Canada

ABSTRACT Seasonal signals of stable isotopes in precipitation, combined with measurements of isotope ratios in soil water, can be used for quantitative estimation of groundwater recharge rates. This study investigates the applicability of using the piston flow principle and the peak shift displacement method to estimate actual groundwater recharge rates in a humid Nordic region located in the province of Quebec, Canada. Two different sites with and without vegetation (C1 and C2) in an unconfined aquifer were tested by measuring soil water isotope ratios (18O/16O and 2H/1H) and volumetric pore water content. Core samples were obtained along the vadose zone down to the groundwater table at the two sites (2.45 m for Site C1 and 4.15 m for Site C2). The peak shift method to estimate groundwater recharge rates was shown to be accurate only in certain specific conditions inherent to the soil properties and the topographical situation of the investigated sites. Indeed, at Site C2, recharge from the snowmelt could not be estimated because of heterogeneity in the lower part of the vadose zone. At this same site the later recharge after the snowmelt (in the period from late spring to early autumn) could be estimated accurately because the upper part of the vadose zone was homogeneous. Furthermore, at site C1, runoff/runon phenomena hampered calculations of actual infiltration and thus produced inaccurate results for recharge. These two different site effects (heterogeneity in the first site and runoff/runon in the other site) were identified as being limiting factors in the accurate assessment of actual recharge. This study therefore recommends the use of the peak shift method for (1) humid Nordic regions, (2) homogeneous and thick vadose zones, and (3) areas with few or limited site effects (runoff/runon).

High resolution monitoring above and below the groundwater table uncovers small – scale hydrochemical gradients

Hydrochemical solute concentrations in the shallow subsurface can be spatially highly variable within small scales, particularly at interfaces. However, most monitoring systems fail to capture these small scale variations. Within this study, we developed a high resolution multilevel well (HR-MLW) with which we monitored water across the interface of the unsaturated and saturated zone with a vertical resolution of 0.05-0.5 m. We installed three of these 4 m deep HR-MLWs in the riparian zone of a third-order river and analyzed for hydrochemical parameters and stable water isotopes. The results showed three distinct vertical zones (unsaturated zone, upper saturated zone, lower saturated zone) within the alluvial aquifer. A 2 m thick layer influenced by river water (upper saturated zone) was not captured by existing monitoring wells with higher screen length. Hydrochemical data (isotopes, total ions) were consistent in all HR-MLWs and showed similar variation over time emphasizing the reliability of the installed monitoring system. Further, the depths zones were also reflected in the NO3-N concentrations; with high spatial variabilities between the three wells. The zonation was constant over time, with seasonal variability in the upper saturated zone due to the influence of river water. This study highlights the use of high resolution monitoring for identifying the spatial and temporal variability of hydrochemical parameters present in many aquifer systems. Possible applications range from riparian zones, agricultural field sites to contaminated site studies, wherever an improved understanding of biogeochemical turnover processes is necessary.