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Dive into the research topics where Christopher J. Mitchell is active.

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Featured researches published by Christopher J. Mitchell.


Nature | 2014

A draft map of the human proteome

Min Sik Kim; Sneha M. Pinto; Derese Getnet; Raja Sekhar Nirujogi; Srikanth S. Manda; Raghothama Chaerkady; Dhanashree S. Kelkar; Ruth Isserlin; Shobhit Jain; Joji Kurian Thomas; Babylakshmi Muthusamy; Pamela Leal-Rojas; Praveen Kumar; Nandini A. Sahasrabuddhe; Lavanya Balakrishnan; Jayshree Advani; Bijesh George; Santosh Renuse; Lakshmi Dhevi N. Selvan; Arun H. Patil; Vishalakshi Nanjappa; Aneesha Radhakrishnan; Samarjeet Prasad; Tejaswini Subbannayya; Rajesh Raju; Manish Kumar; Sreelakshmi K. Sreenivasamurthy; Arivusudar Marimuthu; Gajanan Sathe; Sandip Chavan

The availability of human genome sequence has transformed biomedical research over the past decade. However, an equivalent map for the human proteome with direct measurements of proteins and peptides does not exist yet. Here we present a draft map of the human proteome using high-resolution Fourier-transform mass spectrometry. In-depth proteomic profiling of 30 histologically normal human samples, including 17 adult tissues, 7 fetal tissues and 6 purified primary haematopoietic cells, resulted in identification of proteins encoded by 17,294 genes accounting for approximately 84% of the total annotated protein-coding genes in humans. A unique and comprehensive strategy for proteogenomic analysis enabled us to discover a number of novel protein-coding regions, which includes translated pseudogenes, non-coding RNAs and upstream open reading frames. This large human proteome catalogue (available as an interactive web-based resource at http://www.humanproteomemap.org) will complement available human genome and transcriptome data to accelerate biomedical research in health and disease.


Molecular & Cellular Proteomics | 2012

TSLP Signaling Network Revealed by SILAC-Based Phosphoproteomics

Jun Zhong; Min Sik Kim; Raghothama Chaerkady; Xinyan Wu; Tai Chung Huang; Derese Getnet; Christopher J. Mitchell; Shyam Mohan Palapetta; Jyoti Sharma; Robert N. O'Meally; Robert N. Cole; Akinori Yoda; Albrecht Moritz; Marc Loriaux; John Rush; David M. Weinstock; Jeffrey W. Tyner; Akhilesh Pandey

Thymic stromal lymphopoietin (TSLP) is a cytokine that plays diverse roles in the regulation of immune responses. TSLP requires a heterodimeric receptor complex consisting of IL-7 receptor α subunit and its unique TSLP receptor (gene symbol CRLF2) to transmit signals in cells. Abnormal TSLP signaling (e.g. overexpression of TSLP or its unique receptor TSLPR) contributes to the development of a number of diseases including asthma and leukemia. However, a detailed understanding of the signaling pathways activated by TSLP remains elusive. In this study, we performed a global quantitative phosphoproteomic analysis of the TSLP signaling network using stable isotope labeling by amino acids in cell culture. By employing titanium dioxide in addition to antiphosphotyrosine antibodies as enrichment methods, we identified 4164 phosphopeptides on 1670 phosphoproteins. Using stable isotope labeling by amino acids in cell culture-based quantitation, we determined that the phosphorylation status of 226 proteins was modulated by TSLP stimulation. Our analysis identified activation of several members of the Src and Tec families of kinases including Btk, Lyn, and Tec by TSLP for the first time. In addition, we report TSLP-induced phosphorylation of protein phosphatases such as Ptpn6 (SHP-1) and Ptpn11 (Shp2), which has also not been reported previously. Co-immunoprecipitation assays showed that Shp2 binds to the adapter protein Gab2 in a TSLP-dependent manner. This is the first demonstration of an inducible protein complex in TSLP signaling. A kinase inhibitor screen revealed that pharmacological inhibition of PI-3 kinase, Jak family kinases, Src family kinases or Btk suppressed TSLP-dependent cellular proliferation making them candidate therapeutic targets in diseases resulting from aberrant TSLP signaling. Our study is the first phosphoproteomic analysis of the TSLP signaling pathway that greatly expands our understanding of TSLP signaling and provides novel therapeutic targets for TSLP/TSLPR-associated diseases in humans.


PLOS ONE | 2015

miRge - A Multiplexed Method of Processing Small RNA-Seq Data to Determine MicroRNA Entropy

Alexander S. Baras; Christopher J. Mitchell; Jason R. Myers; Simone Gupta; Lien Chun Weng; John M. Ashton; Toby C. Cornish; Akhilesh Pandey; Marc K. Halushka

Small RNA RNA-seq for microRNAs (miRNAs) is a rapidly developing field where opportunities still exist to create better bioinformatics tools to process these large datasets and generate new, useful analyses. We built miRge to be a fast, smart small RNA-seq solution to process samples in a highly multiplexed fashion. miRge employs a Bayesian alignment approach, whereby reads are sequentially aligned against customized mature miRNA, hairpin miRNA, noncoding RNA and mRNA sequence libraries. miRNAs are summarized at the level of raw reads in addition to reads per million (RPM). Reads for all other RNA species (tRNA, rRNA, snoRNA, mRNA) are provided, which is useful for identifying potential contaminants and optimizing small RNA purification strategies. miRge was designed to optimally identify miRNA isomiRs and employs an entropy based statistical measurement to identify differential production of isomiRs. This allowed us to identify decreasing entropy in isomiRs as stem cells mature into retinal pigment epithelial cells. Conversely, we show that pancreatic tumor miRNAs have similar entropy to matched normal pancreatic tissues. In a head-to-head comparison with other miRNA analysis tools (miRExpress 2.0, sRNAbench, omiRAs, miRDeep2, Chimira, UEA small RNA Workbench), miRge was faster (4 to 32-fold) and was among the top-two methods in maximally aligning miRNAs reads per sample. Moreover, miRge has no inherent limits to its multiplexing. miRge was capable of simultaneously analyzing 100 small RNA-Seq samples in 52 minutes, providing an integrated analysis of miRNA expression across all samples. As miRge was designed for analysis of single as well as multiple samples, miRge is an ideal tool for high and low-throughput users. miRge is freely available at http://atlas.pathology.jhu.edu/baras/miRge.html.


Molecular & Cellular Proteomics | 2014

Annotation of the Zebrafish Genome through an Integrated Transcriptomic and Proteomic Analysis

Dhanashree S. Kelkar; Elayne Provost; Raghothama Chaerkady; Babylakshmi Muthusamy; Srikanth S. Manda; Tejaswini Subbannayya; Lakshmi Dhevi N. Selvan; Chieh-Huei Wang; Keshava K. Datta; Sunghee Woo; Sutopa B. Dwivedi; Santosh Renuse; Derese Getnet; Tai Chung Huang; Min-Sik Kim; Sneha M. Pinto; Christopher J. Mitchell; Praveen Kumar; Jyoti Sharma; Jayshree Advani; Gourav Dey; Lavanya Balakrishnan; Nazia Syed; Vishalakshi Nanjappa; Yashwanth Subbannayya; Renu Goel; T. S. Keshava Prasad; Vineet Bafna; Ravi Sirdeshmukh; Harsha Gowda

Accurate annotation of protein-coding genes is one of the primary tasks upon the completion of whole genome sequencing of any organism. In this study, we used an integrated transcriptomic and proteomic strategy to validate and improve the existing zebrafish genome annotation. We undertook high-resolution mass-spectrometry-based proteomic profiling of 10 adult organs, whole adult fish body, and two developmental stages of zebrafish (SAT line), in addition to transcriptomic profiling of six organs. More than 7,000 proteins were identified from proteomic analyses, and ∼69,000 high-confidence transcripts were assembled from the RNA sequencing data. Approximately 15% of the transcripts mapped to intergenic regions, the majority of which are likely long non-coding RNAs. These high-quality transcriptomic and proteomic data were used to manually reannotate the zebrafish genome. We report the identification of 157 novel protein-coding genes. In addition, our data led to modification of existing gene structures including novel exons, changes in exon coordinates, changes in frame of translation, translation in annotated UTRs, and joining of genes. Finally, we discovered four instances of genome assembly errors that were supported by both proteomic and transcriptomic data. Our study shows how an integrative analysis of the transcriptome and the proteome can extend our understanding of even well-annotated genomes.


Genome Research | 2017

Toward the human cellular microRNAome

Matthew N. McCall; Min Sik Kim; Mohammed Adil; Arun H. Patil; Yin Lu; Christopher J. Mitchell; Pamela Leal-Rojas; Jinchong Xu; Manoj Kumar; Valina L. Dawson; Ted M. Dawson; Alexander S. Baras; Avi Z. Rosenberg; Dan E. Arking; Kathleen H. Burns; Akhilesh Pandey; Marc K. Halushka

MicroRNAs are short RNAs that serve as regulators of gene expression and are essential components of normal development as well as modulators of disease. MicroRNAs generally act cell-autonomously, and thus their localization to specific cell types is needed to guide our understanding of microRNA activity. Current tissue-level data have caused considerable confusion, and comprehensive cell-level data do not yet exist. Here, we establish the landscape of human cell-specific microRNA expression. This project evaluated 8 billion small RNA-seq reads from 46 primary cell types, 42 cancer or immortalized cell lines, and 26 tissues. It identified both specific and ubiquitous patterns of expression that strongly correlate with adjacent superenhancer activity. Analysis of unaligned RNA reads uncovered 207 unknown minor strand (passenger) microRNAs of known microRNA loci and 495 novel putative microRNA loci. Although cancer cell lines generally recapitulated the expression patterns of matched primary cells, their isomiR sequence families exhibited increased disorder, suggesting DROSHA- and DICER1-dependent microRNA processing variability. Cell-specific patterns of microRNA expression were used to de-convolute variable cellular composition of colon and adipose tissue samples, highlighting one use of these cell-specific microRNA expression data. Characterization of cellular microRNA expression across a wide variety of cell types provides a new understanding of this critical regulatory RNA species.


BMC Systems Biology | 2015

A multi-omic analysis of human naïve CD4+ T cells

Christopher J. Mitchell; Derese Getnet; Min Sik Kim; Srikanth S. Manda; Praveen Kumar; Tai Chung Huang; Sneha M. Pinto; Raja Sekhar Nirujogi; Mio Iwasaki; Patrick G. Shaw; Xinyan Wu; Jun Zhong; Raghothama Chaerkady; Arivusudar Marimuthu; Babylakshmi Muthusamy; Nandini A. Sahasrabuddhe; Rajesh Raju; Caitlyn E. Bowman; Ludmila Danilova; Jevon Cutler; Dhanashree S. Kelkar; Charles G. Drake; T. S. Keshava Prasad; Luigi Marchionni; Peter Murakami; Alan F. Scott; Leming Shi; Jean Thierry-Mieg; Danielle Thierry-Mieg; Rafael A. Irizarry

BackgroundCellular function and diversity are orchestrated by complex interactions of fundamental biomolecules including DNA, RNA and proteins. Technological advances in genomics, epigenomics, transcriptomics and proteomics have enabled massively parallel and unbiased measurements. Such high-throughput technologies have been extensively used to carry out broad, unbiased studies, particularly in the context of human diseases. Nevertheless, a unified analysis of the genome, epigenome, transcriptome and proteome of a single human cell type to obtain a coherent view of the complex interplay between various biomolecules has not yet been undertaken. Here, we report the first multi-omic analysis of human primary naïve CD4+ T cells isolated from a single individual.ResultsIntegrating multi-omics datasets allowed us to investigate genome-wide methylation and its effect on mRNA/protein expression patterns, extent of RNA editing under normal physiological conditions and allele specific expression in naïve CD4+ T cells. In addition, we carried out a multi-omic comparative analysis of naïve with primary resting memory CD4+ T cells to identify molecular changes underlying T cell differentiation. This analysis provided mechanistic insights into how several molecules involved in T cell receptor signaling are regulated at the DNA, RNA and protein levels. Phosphoproteomics revealed downstream signaling events that regulate these two cellular states. Availability of multi-omics data from an identical genetic background also allowed us to employ novel proteogenomics approaches to identify individual-specific variants and putative novel protein coding regions in the human genome.ConclusionsWe utilized multiple high-throughput technologies to derive a comprehensive profile of two primary human cell types, naïve CD4+ T cells and memory CD4+ T cells, from a single donor. Through vertical as well as horizontal integration of whole genome sequencing, methylation arrays, RNA-Seq, miRNA-Seq, proteomics, and phosphoproteomics, we derived an integrated and comparative map of these two closely related immune cells and identified potential molecular effectors of immune cell differentiation following antigen encounter.


Genomics | 2016

Long non-coding RNA expression in primary human monocytes.

Hoda Mirsafian; Srinivas Srikanth Manda; Christopher J. Mitchell; Sreelakshmi Sreenivasamurthy; Adiratna Mat Ripen; Saharuddin B. Mohamad; Amir Feisal Merican; Akhilesh Pandey

Long non-coding RNAs (lncRNAs) have been shown to possess a wide range of functions in both cellular and developmental processes including cancers. Although some of the lncRNAs have been implicated in the regulation of the immune response, the exact function of the large majority of lncRNAs still remains unknown. In this study, we characterized the lncRNAs in human primary monocytes, an essential component of the innate immune system. We performed RNA sequencing of monocytes from four individuals and combined our data with eleven other publicly available datasets. Our analysis led to identification of ~8000 lncRNAs of which >1000 have not been previously reported in monocytes. PCR-based validation of a subset of the identified novel long intergenic noncoding RNAs (lincRNAs) revealed distinct expression patterns. Our study provides a landscape of lncRNAs in monocytes, which could facilitate future experimental studies to characterize the functions of these molecules in the innate immune system.


Leukemia | 2017

Differential signaling through p190 and p210 BCR-ABL fusion proteins revealed by interactome and phosphoproteome analysis

Jevon Cutler; R. Tahir; Sreelakshmi K. Sreenivasamurthy; Christopher J. Mitchell; Santosh Renuse; Raja Sekhar Nirujogi; Arun H. Patil; Mohammad Heydarian; X. Wong; Xinyan Wu; Tai Chung Huang; Min Sik Kim; Akhilesh Pandey

Two major types of leukemogenic BCR-ABL fusion proteins are p190BCR-ABLand p210BCR-ABL. Although the two fusion proteins are closely related, they can lead to different clinical outcomes. A thorough understanding of the signaling programs employed by these two fusion proteins is necessary to explain these clinical differences. We took an integrated approach by coupling protein–protein interaction analysis using biotinylation identification with global phosphorylation analysis to investigate the differences in signaling between these two fusion proteins. Our findings suggest that p190BCR-ABL and p210BCR-ABL differentially activate important signaling pathways, such as JAK-STAT, and engage with molecules that indicate interaction with different subcellular compartments. In the case of p210BCR-ABL, we observed an increased engagement of molecules active proximal to the membrane and in the case of p190BCR-ABL, an engagement of molecules of the cytoskeleton. These differences in signaling could underlie the distinct leukemogenic process induced by these two protein variants.


Molecular & Cellular Proteomics | 2016

PyQuant: A Versatile Framework for Analysis of Quantitative Mass Spectrometry Data

Christopher J. Mitchell; Min Sik Kim; Chan Hyun Na; Akhilesh Pandey

Quantitative mass spectrometry data necessitates an analytical pipeline that captures the accuracy and comprehensiveness of the experiments. Currently, data analysis is often coupled to specific software packages, which restricts the analysis to a given workflow and precludes a more thorough characterization of the data by other complementary tools. To address this, we have developed PyQuant, a cross-platform mass spectrometry data quantification application that is compatible with existing frameworks and can be used as a stand-alone quantification tool. PyQuant supports most types of quantitative mass spectrometry data including SILAC, NeuCode, 15N, 13C, or 18O and chemical methods such as iTRAQ or TMT and provides the option of adding custom labeling strategies. In addition, PyQuant can perform specialized analyses such as quantifying isotopically labeled samples where the label has been metabolized into other amino acids and targeted quantification of selected ions independent of spectral assignment. PyQuant is capable of quantifying search results from popular proteomic frameworks such as MaxQuant, Proteome Discoverer, and the Trans-Proteomic Pipeline in addition to several standalone search engines. We have found that PyQuant routinely quantifies a greater proportion of spectral assignments, with increases ranging from 25–45% in this study. Finally, PyQuant is capable of complementing spectral assignments between replicates to quantify ions missed because of lack of MS/MS fragmentation or that were omitted because of issues such as spectra quality or false discovery rates. This results in an increase of biologically useful data available for interpretation. In summary, PyQuant is a flexible mass spectrometry data quantification platform that is capable of interfacing with a variety of existing formats and is highly customizable, which permits easy configuration for custom analysis.


Journal of Proteomics & Bioinformatics | 2014

Prediction of Gene Activity in Early B Cell Development Based on an Integrative Multi-Omics Analysis

Mohammad Heydarian; Teresa R Luperchio; Jevon Cutler; Christopher J. Mitchell; Min Sik Kim; Akhilesh Pandey; Barbara Sollner-Webb

An increasingly common method for predicting gene activity is genome-wide chromatin immuno-precipitation of ‘active’ chromatin modifications followed by massively parallel sequencing (ChIP-seq). In order to understand better the relationship between developmentally regulated chromatin landscapes and regulation of early B cell development, we determined how differentially active promoter regions were able to predict relative RNA and protein levels at the pre-pro-B and pro-B stages. Herein, we describe a novel ChIP-seq quantification method (cRPKM) to identify active promoters and a multi-omics approach that compares promoter chromatin status with ongoing active transcription (GRO-seq), steady state mRNA (RNA-seq), inferred mRNA stability, and relative proteome abundance measurements (iTRAQ). We demonstrate that active chromatin modifications at promoters are good indicators of transcription and steady state mRNA levels. Moreover, we found that promoters with active chromatin modifications exclusively in one of these cell states frequently predicted the differential abundance of proteins. However, we found that many genes whose promoters have non-differential but active chromatin modifications also displayed changes in abundance of their cognate proteins. As expected, this large class of developmentally and differentially regulated proteins that was uncoupled from chromatin status used mostly post-transcriptional mechanisms. Strikingly, the most differentially abundant protein in our B-cell development system, 2410004B18Rik, was regulated by a post-transcriptional mechanism, which further analyses indicated was mediated by a micro-RNA. These data highlight how this integrated multi-omics data set can be a useful resource in uncovering regulatory mechanisms. This data can be accessed at: https://usegalaxy.org/u/thereddylab/p/prediction-of-gene-activity-based-on-an-integrative-multi-omics-analysis

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Akhilesh Pandey

Johns Hopkins University School of Medicine

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Min Sik Kim

Johns Hopkins University School of Medicine

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Raghothama Chaerkady

Johns Hopkins University School of Medicine

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Tai Chung Huang

Johns Hopkins University School of Medicine

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Derese Getnet

Johns Hopkins University

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