Christopher S. Brown

Surface roughness, asperity contact and gold RF MEMS switch behavior

Modeling predictions and experimental measurements were obtained to characterize the electro-mechanical response of radio frequency (RF) microelectromechanical (MEM) switches due to variations in surface roughness and finite asperity deformations. Three-dimensional surface roughness profiles were generated, based on a Weierstrass–Mandelbrot fractal representation, to match the measured roughness characteristics of contact bumps of manufactured RF MEMS switches. Contact asperity deformations due to applied contact pressures were then obtained by a creep constitutive formulation. The contact pressure is derived from the interrelated effects of roughness characteristics, material hardening and softening, temperature increases due to Joule heating and contact forces. This modeling framework was used to understand how contact resistance evolves due to changes in the real contact area, the number of asperities in contact, and the temperature and resistivity profiles at the contact points. The numerical predictions were qualitatively consistent with the experimental measurements and observations of how contact resistance evolves as a function of deformation time history. This study provides a framework that is based on integrated modeling and experimental measurements, which can be used in the design of reliable RF MEMS devices with extended life cycles.

Arabidopsis thaliana calcium-dependent lipid-binding protein (AtCLB): a novel repressor of abiotic stress response

Ca(2+) is an important second messenger in plant signal transduction pathways regulating stress-induced gene expression. Functional analysis of plant proteins containing Ca(2+)-binding domains (C2 domains) will help us understand the mechanisms behind the role of transcriptional regulators in the Ca(2+) signalling pathway and open new perspectives for crop genetic improvement. We identified a novel transcriptional regulator, a Ca(2+)-dependent lipid-binding protein (AtCLB) containing a C2 domain. AtCLB binds specifically to the promoter of the Arabidopsis thalianol synthase gene (AtTHAS1), whose expression is induced by gravity and light. Here we describe the role of the Atclb gene encoding the AtCLB protein. Expression of the Atclb gene was documented in all analysed tissues of Arabidopsis (leaf, root, stem, flower, and silique) by real-time PCR analysis. Immunofluorescence analysis revealed that AtCLB protein is localized in the nucleus of cells in Arabidopsis root tips. We demonstrated that the AtCLB protein was capable of binding to the membrane lipid ceramide. The role of the Atclb gene in negatively regulating responses to abiotic stress in Arabidopsis thaliana was identified. The loss of the Atclb gene function confers an enhanced drought and salt tolerance and a modified gravitropic response in T-DNA insertion knockout mutant lines. Expression of AtTHAS1 in Atclb knockout mutant lines was increased compared with wild type and a 35S-Atclb overexpression line suggesting AtCLB as a transcriptional repressor of AtTHAS1.

Increasing inositol (1,4,5)-trisphosphate metabolism affects drought tolerance, carbohydrate metabolism and phosphate-sensitive biomass increases in tomato

Inositol-(1,4,5)-trisphosphate (InsP(3)) is a second messenger in plants that increases in response to many stimuli. The metabolic consequences of this signalling pathway are not known. We reduced the basal level of InsP(3) in tomato (Solanum lycopersicum cv. Micro-Tom) by expressing the human type I inositol polyphosphate 5-phosphatase (InsP 5-ptase) gene. Transgenic lines producing InsP 5-ptase protein had between 15% and 30% of the basal InsP(3) level of control plants. This increased hydrolysis of InsP(3) caused dramatic increases in drought tolerance, vegetative biomass and lycopene and hexose concentrations in the fruits. Transcript profiling of root, leaf and fruit tissues identified a small group of genes, including a cell-wall invertase inhibitor gene, that were differentially regulated in all tissues of the InsP 5-ptase expressing plants. Significant differences were found in the amounts of carbohydrates and organic phosphate in these plants. Plants with increased hydrolysis of InsP(3) in the cytosol also showed increased net CO(2)-fixation and sucrose export into sink tissue and storage of hexoses in the source leaves. The increase in biomass was dependent on the supply of inorganic phosphate in the nutrient medium. Uptake and storage of phosphate was increased in the transgene expressing lines. This suggests that in tomato, increased flux through the inositol phosphate pathway uncoupled phosphate sensing from phosphate metabolism. Altering the second messenger, InsP(3), revealed multiple coordinated changes in development and metabolism in tomato that have potential for crop improvement.

Temperature dependence of asperity contact and contact resistance in gold RF MEMS switches

Experimental measurements and modeling predictions were obtained to characterize the electro-mechanical response of two different gold contact radio frequency microelectromechanical system (RF MEMS) switches due to variations in the temperature and applied contact voltage. A three-dimensional surface roughness profile from AFM measurements of the top contact surface of a sample RF MEMS switch was used to obtain modeling predictions of the time-dependent deformation of the asperity microcontacts, real areas of contact, number of asperity microcontacts and constriction resistance. The experimental data indicated a decrease in the overall resistance and a decrease in the creep mechanism at 77 K and 5.6 K when compared to measurements at 293 K. At 293 K, there is more contact area per unit time, and the resistance drop from the increase in real contact area dominates the resistance increase due to asperity heating. At 77 K, the creep rate is reduced, and fewer asperities are in contact. At 5.6 K, the change in contact area over time is small, and the contact resistance measurement is dominated by the Joule heating. The data presented and constriction resistance modeling for gold RF MEMS switches show that temperature plays a significant role in the creep deformation and heating of switch contacts.

Role of inositol 1,4,5‐triphosphate signalling in gravitropic and phototropic gene expression

Plants sense light and gravity to orient their direction of growth. One common component in the early events of both phototropic and gravitropic signal transduction is activation of phospholipase C (PLC), which leads to an increase in inositol 1,4,5-triphosphate (InsP(3)) levels. The InsP(3) signal is terminated by hydrolysis of InsP(3) through inositolpolyphosphate-5-phosphatases (InsP 5-ptases). Arabidopsis plants expressing a heterologous InsP 5-ptase have low basal InsP(3) levels and exhibit reduced gravitropic and phototropic bending. Downstream effects of InsP(3)-mediated signalling are not understood. We used comparative transcript profiling to characterize gene expression changes in gravity- or light-stimulated Arabidopsis root apices that were manipulated in their InsP(3) metabolism either through inhibition of PLC activity or expression of InsP 5-ptase. We identified InsP(3)-dependent and InsP(3)-independent co-regulated gene sets in response to gravity or light stimulation. Inhibition of PLC activity in wild-type plants caused similar changes in transcript abundance in response to gravitropic and phototropic stimulation as in the transgenic lines. Therefore, we conclude that changes in gene expression in response to gravitropic and phototropic stimulation are mediated by two signal transduction pathways that vary in their dependence on changes in InsP(3).

The role of creep in the time-dependent resistance of Ohmic gold contacts in radio frequency microelectromechanical system devices

It is shown that measured and calculated time-dependent electrical resistances of closed gold Ohmic switches in radio frequency microelectromechanical system (rf-MEMS) devices are well described by a power law that can be derived from a single asperity creep model. The analysis reveals that the exponent and prefactor in the power law arise, respectively, from the coefficient relating creep rate to applied stress and the initial surface roughness. The analysis also shows that resistance plateaus are not, in fact, limiting resistances but rather result from the small coefficient in the power law. The model predicts that it will take a longer time for the contact resistance to attain a power law relation with each successive closing of the switch due to asperity blunting. Analysis of the first few seconds of the measured resistance for three successive openings and closings of one of the MEMS devices supports this prediction. This work thus provides guidance toward the rational design of Ohmic contacts with ...

ROSY1, a novel regulator of gravitropic response is a stigmasterol binding protein

The gravitropic bending in plant roots is caused by asymmetric cell elongation. This requires an asymmetric increase in cell surface and therefore plasma membrane components such as lipids, sterols, and membrane proteins. We have identified an early gravity-regulated protein in Arabidopsis thaliana root apices that binds stigmasterol and phosphoethanolamines. This root-specific protein interacts with the membrane transport protein synaptotagmin-1 and was therefore named InteractoR Of SYnaptotagmin1 (ROSY1). While interactions between ML-domain proteins with membrane transport proteins and their impact have been reported from animal cell systems, this is the first report of such an interaction in a plant system. Homozygous mutants of ROSY1 exhibit decreased basipetal auxin transport, a faster root gravitropic response, and an increase in salt stress tolerance. Our results suggest that ROSY1 plays a role in root gravitropism, possibly by facilitating membrane trafficking and asymmetric cell elongation via its interaction with synaptotagmin-1.

How software users recommend tools to each other

To help users gain awareness of tools and features available in applications, recommender systems can automatically suggest useful tools. Such systems aim to present recommendations just like users would recommend tools to one another, but little is known about the nature of these user-to-user recommendations. This paper explores user-to-user recommendations through a study of 13 pairs of software users performing data analysis tasks. We found that users were more likely to adopt tools when they were receptive to the recommendation, but did not find the recommendations were any more likely to be effective when they contained other characteristics such as politeness, persuasiveness, or referred to observable tools. These findings suggest that, for example, automated systems should avoid recommending obscure and unfamiliar tools, but making recommendations politely is not a critical design goal.

Flower: Navigating program flow in the IDE

Program navigation is a critical task for software developers. State-of-the-art tools have been shown to support effective program navigation strategies, and do so by adding widgets, secondary views, and visualizations to the screen. In this work, we build on prior work by exploring what types of navigation can be supported with relatively few interface elements. To that end, we designed and implemented a prototype tool, named Flower, that supports structural program navigation while maintaining a minimalistic interface. Flower enables developers to simultaneously navigate control flow and data flow within the Eclipse Integrated Development Environment. Based on a preliminary evaluation with eight programmers, Flower succeeds when call graphs contained relatively few branches, but was strained by complex program structures.

Analysis of Cycle Lifetimes and Failure Modes for RF MEMS Switches

RF MEMS switch lifetimes are limited by stiction of the moving components and degradation of the metal to metal contact points during cycling. Currently, maximum switch lifetimes are around 10 to 25 billion cycles. Past experimentation has shown that some stiction problems can be overcome by carefully controlling the operating parameters, but problems at the contact points remain [1]. It is believed that by developing a set of tribological design rules which limit the factors leading to catastrophic failure, switches can operate in excess of 100 billion cycles. Recent research has quantified the reliability and durability of gold contact points on RF MEMS switches as a function of current [2]. Most experimentation on RF MEMS switches has focused on controlling the operating parameters such as current, voltage, electrode materials, contact area, switching mode and force; however, limited work has been performed on a single device type in multiple environmentally controlled testing conditions such as vacuum, cryogenic temperatures, etc. This presentation will discuss performance of the wiSpry RF MEMS switch focusing on quantification of device reliability and failure mechanisms under various atmospheric and temperature conditions. Environmental testing conditions include switching in open air, vacuum and inert gasses, in temperatures ranging from 294 K to 4 K.© 2005 ASME