Chu-An Chang

Hybridization properties of the 5-methyl-isocytidine/isoguanosine base pair in synthetic oligodeoxynucleotides

Abstract The hybridization properties of the 5-methyl-isocytidine/isoguanosine base pair in synthetic oligodeoxynucleotides have been investigated. The 5-methyl-isocytidine/is oguanosine base pair has been determined to be isoenergetic with the cytidine/guanosine, and each base can effectively discriminate mismatches. The synthesis of oligodeoxynucleotides containing 5-methyl-2′-deoxyisocytidine was improved using N 2 -((di-n-butylamino)methylene) protection.

Construction of branched DNA (bDNA) molecules by chemical ligation

Abstract Chemical ligation has been investigated in the assembly of branched DNA (bDNA) molecules. Water soluble coupling reagents were used to ligate single-stranded linear oligomers to bDNA molecules containing 5 and 15 branches. The ligations were particularly efficient when one of the oligomers was partially double-stranded constructed by branching to create a terminal ligation linker (AutoLink). Branched DNA molecules containing more than 1000 nucleotides were assembled.

HYBRIDIZATION PROPERTIES OF THE 5-METHYL-ISOCYTIDINE/ISOGUANOSINE BASE PAIR IN SYNTHETIC OLIGODEOXYNUCLEOTIDES

Abstract The hybridization properties of the 5-methyl-isocytidine/isoguanosine base pair in synthetic oligodeoxynucleotides have been investigated. The 5-methyl-isocytidine/isoguanosine base pair has been determined to be isoenergetic with the cytidine/guanosine, and each base can effectively discriminate mismatches.

An Improved Deprotection Procedure of Amine-Containing Oligonucleotides from Acrylonitrile Modification

Abstract Alkylation of alkylamine by acrylonitrile via Michael addition1 occurred when standard ammonium hydroxide deprotection was used. The alkylation was greatly reduced using an improved two-step procedure when BCE phosphotriester protecting groups were selectively removed with t-butylamine prior to ammonium hydroxide deprotection.