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Dive into the research topics where Chuanfeng Zhou is active.

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Featured researches published by Chuanfeng Zhou.


Medical Science Monitor | 2012

Hydrogen-rich saline protects immunocytes from radiation-induced apoptosis.

Yanyong Yang; Bailong Li; Cong Liu; Yunhai Chuai; Jixiao Lei; Fu Gao; Jianguo Cui; Ding Hui Sun; Ying Cheng; Chuanfeng Zhou; Jianming Cai

Summary Background Radiation often causes depletion of immunocytes in tissues and blood, which results in immunosuppression. Molecular hydrogen (H2) has been shown in recent studies to have potential as a safe and effective radioprotective agent through scavenging free radicals. This study was designed to test the hypothesis that H2 could protect immunocytes from ionizing radiation (IR). Material/Methods H2 was dissolved in physiological saline or medium using an apparatus produced by our department. A 2-[6-(4′-hydroxy) phenoxy-3H-xanthen-3-on-9-yl] benzoate (HPF) probe was used to detect intracellular hydroxyl radicals (•OH). Cell apoptosis was evaluated by annexin V-FITC and Propidium iodide (PI) staining as well as the caspase 3 activity. Finally, we examined the hematological changes using an automatic Sysmex XE 2100 hematology analyzer. Results We demonstrated H2-rich medium pretreatment reduced •OH level in AHH-1 cells. We also showed H2 reduced radiation-induced apoptosis in thymocytes and splenocytes in living mice. Radiation-induced caspase 3 activation was also attenuated by H2 treatment. Finally, we found that H2 rescued the radiation-caused depletion of white blood cells (WBC) and platelets (PLT). Conclusions This study suggests that H2 protected the immune system and alleviated the hematological injury induced by IR.


Scientific Reports | 2015

A critical role of toll-like receptor 2 (TLR2) and its’ in vivo ligands in radio-resistance

Fu Gao; Chaoxiong Zhang; Chuanfeng Zhou; Weimin Sun; Xin Liu; Pei Zhang; Jiaqi Han; Linfeng Xian; Dongchen Bai; Hu Liu; Ying Cheng; Bailong Li; Jianguo Cui; Jianming Cai; Cong Liu

The role of Toll-like receptor-2 (TLR2) in radio-resistance remained largely unknown. TLR2 knockout (TLR2−/−) mice received radiation of 6.5 Gy, and then were studied. We found that radiation resulted in more severe mortality and morbidity rates in TLR2−/− mice. The cause of death in TLR2−/− mice may be severe and persistent bone marrow cell loss. Injection of the TLR2 agonist Pam3CSK4 into wild type (WT) mice induced radio-resistance. Myd88−/− mice were more susceptible to radiation. In conclusion, our data indicate that, similar to TLR4, TLR2 plays a critical role in radio-resistance.


Journal of Cellular Biochemistry | 2012

Mangiferin aglycone attenuates radiation-induced damage on human intestinal epithelial cells†‡

Jixiao Lei; Chuanfeng Zhou; Honggang Hu; Lina Hu; Meijia Zhao; Yanyong Yang; Yunhai Chuai; Jin Ni; Jianming Cai

Recent studies suggest that mangiferin aglycone (norathyriol) has great potential as a novel radioprotector without any known toxic side effects. In this study, we assessed the protective effects of mangiferin aglycone against radiation‐induced injuries on normal human intestinal epithelial cells (HIECs), while using mangiferin as a reference compound. The in vitro experiments showed that pretreatment of either mangiferin aglycone or mangiferin could inhibit cytotoxic effects of ionizing irradiation (IR) on HIECs. Cellular changes were estimated by measuring cell viability, clonogenic surviving rate, and apoptotic rate. Compared to mangiferin, we found mangiferin aglycone had greater radioprotective effects of mangiferin aglycone on HIECs. It has been demonstrated that the cytotoxicity of ionizing radiation relates to its capacity to induce DNA damage. In view of this, we monitored DNA double‐strand breaks (DSBs) using γH2AX foci formation to test whether mangiferin aglycone and mangiferin could modulate genotoxic effects of radiation. It shows that mangiferin aglycone could eliminate 46.8% of the total DSBs of the cells exposed to 2 Gy IR, which is significantly better than mangiferin. Complementing earlier results from our group, it appears possible to conclude that mangiferin aglycone presents potential useful effects on IR‐induced damage and may be a better radioprotective agent than mangiferin therapeutically. J. Cell. Biochem. 113: 2633–2642, 2012.


Free Radical Research | 2013

Protective effects of carboxyfullerene in irradiated cells and BALB/c mice

Jianguo Cui; Y. Yang; Y. Cheng; Fu Gao; Cong Liu; Chuanfeng Zhou; Bailong Li; Jianmei Cai

Abstract It has been reported that carboxyfullerene (C3) exhibits a strong free radical scavenging capacity, which plays a critical role in radiation damage. We hypothesized that C3 is an effective radioprotective agent. In this study, we demonstrated that C3 effectively scavenged hydroxyl radical in a cell-free Fenton system and that C3 showed no obvious toxicity for cultured cells. Different concentrations (100–400 mg/L) of C3 pretreatment effectively protected AHH-1 cells from radiation-induced apoptosis, by about 2-folds, while only 100 mg/L of C3 reduced the apoptosis rate of human intestinal crypt epithelial cells (HIECs). C3 also alleviated DNA damage detected using comet assay at 0, 4, and 8 h after irradiation. It was found that 75% of mice were protected from 7.2 Gy γ-irradiation-induced death when 100 mg/kg of C3 was administered prior to irradiation, but no change was observed for the survival time of mice which died. We also found that C3 attenuated radiation-mediated decreases in endogenous antioxidants such as SOD and GSH and reduced the level of MDA. In conclusion, these data showed that C3 effectively protected cells and mice from radiation injury, thus indicating the potential of C3 as a safe and effective radioprotectant.


Basic & Clinical Pharmacology & Toxicology | 2011

The mechanism for the ameliorative effect of CpG-oligodeoxynucleotides on bone marrow hemopoiesis radiation injury.

Chao Zhang; Jin Ni; Fu Gao; Ding Sun; Chuanfeng Zhou; Ying Cheng; Jianming Cai; Bailong Li

Bone marrow is a major site of radiation injury. The extreme sensitivity of bone marrow cells to genotoxic stress largely determines the adverse side effects of radiation. CpG-oligodeoxynucleotide (ODN) is known to be radioprotective in extramedullary hemopoiesis, but its effect on bone marrow hemopoiesis remains unknown. In this study, we investigated whether CpG-ODN ameliorated hemopoiesis radiation injury when administered after total-body irradiation (TBI). Mice were treated with 50 μg of CpG-ODN via intraperitoneal injection (i.p) 30 min., 24 and 48 hr after TBI. Our results show that CpG-ODN was able to mediate the activation of nuclear factor κB (NF-κB) via degradation of inhibitor NF-κB (IκB-α), and some oxidative stress parameters (malondialdehyde, glutathione and superoxide dismutase) showed significant differences between the radiation control group and the radiation and administration of CpG-ODN group. White blood cell count, bone marrow cell count and bone marrow histological examination indicated that CpG-ODN minimized bone marrow damage induced by radiation. Exogenous colony-forming unit-spleen count indicated that CpG-ODN reduced primitive hemopoietic stem cell damage and reconstituted the hemopoietic system after TBI. The survival of mice was also enhanced after various levels of TBI. The calculated dose reduction factor was 1.2. Thus, we conclude that CpG-ODN may contribute to the amelioration of hemopoiesis radiation injury.


International Journal of Biological Sciences | 2011

MiR-34a in age and tissue related radio-sensitivity and serum miR-34a as a novel indicator of radiation injury.

Cong Liu; Chuanfeng Zhou; Fu Gao; Shengyun Cai; Chao Zhang; Luqian Zhao; Fang Zhao; Fei Cao; Jing Lin; Yanyong Yang; Jin Ni; Jun Jia; Wei Wu; Li Zhou; Jianguo Cui; Wei Zhang; Bailong Li; Jianming Cai


International Journal of Biological Sciences | 2011

Hydrogen Protects Mice from Radiation Induced Thymic Lymphoma in BALB/c Mice

Luqian Zhao; Chuanfeng Zhou; Jian Zhang; Fu Gao; Bailong Li; Yunhai Chuai; Cong Liu; Jianming Cai


Oncotarget | 2014

MTERFD1 functions as an oncogene

Chaoxiong Zhang; Ning Wu; Fu Gao; Jiaqi Han; Yanyong Yang; Chuanfeng Zhou; Weimin Sun; Linfeng Xian; Ying Cheng; Bailong Li; Jianming Cai; Cong Liu


Academic Journal of Second Military Medical University | 2011

Effect of irradiation-induced miR-34a expression on irradiation-sensitivity of cells: Effect of irradiation-induced miR-34a expression on irradiation-sensitivity of cells

Ying Cheng; Sheng-hui He; Cong Liu; Chuanfeng Zhou; Jianguo Cui; Ding Sun; Jian-ming Cai; Bai-long Li; Fu Gao


Academic Journal of Second Military Medical University | 2011

Protective effects of carboxyfullerene C 3 against γ-ray irradiation injury in mice: Protective effects of carboxyfullerene C 3 against γ-ray irradiation injury in mice

Jianguo Cui; Ying Cheng; Fu Gao; Ding Sun; Chuanfeng Zhou; Hao Jiang; Jian-ming Cai; Bai-long Li

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Fu Gao

Second Military Medical University

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Jianguo Cui

Second Military Medical University

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Ying Cheng

Second Military Medical University

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Bailong Li

Second Military Medical University

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Jianming Cai

Second Military Medical University

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Ding Sun

Second Military Medical University

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Yanyong Yang

Second Military Medical University

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Jian-ming Cai

Second Military Medical University

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Chao Zhang

Second Military Medical University

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