Chung-Youl Park

First report of Maize yellow mosaic virus infecting Panicum miliaceum and Sorghum bicolor in South Korea

Maize yellow mosaic virus (MaYMV) is a tentative new Polerovirus, which was recently identified from maize (Zea mays) in China (Chen et al. 2016). MaYMV has also been reported to infect sugarcane (Saccharum spp.) and itch grass (Rottboellia cochinchinensis), and it has been reported in Asia, Africa and South America (Goncalves et al. 2017; Palanga et al. 2017; Yahaya et al. 2017). In this study, MaYMV was detected in Panicum miliaceum and Sorghum bicolor using Illumina HiSeq2500 system by Theragen Etex Bio Institute (Suwon, Korea) and SG-VIPdb by SeqGenesis (Daejeon, Korea), as described by Lim et al. (2015). Twenty samples of P. miliaceum and sixty three samples of S. bicolor were collected from July to September and from June to October, 2016, respectively, in South Korea. The high-throughput RNA sequencing of the samples mixed into one pool resulted in a single large contig (5606-nt) with nearly complete MaYMV genome coverage. The contig was assembled from a total of 234,537 reads; the maximum, minimum...

First Report of Soybean mosaic virus and Soybean yellow mottle mosaic virus in Vigna angularis

In 2015 and 2016, 203 samples of adzuki bean (Vigna angularis) exhibiting viral disease-like symptoms, such as mosaic, mottle, yellowing, dwarfing and leaf rolling, were collected from 18 cities/counties in Korea. Total RNA was prepared from a pooled sample using easy-spinTM Total RNA Extraction Kit (iNtRON Biotechnology, Seongnam, Korea) and used for high-throughput paired-end RNA sequencing. Raw data analysis was conducted by Illumina HiSeq 2500 System at the Theragen Etex Bio Institute (Suwon, Korea) and SG-VIPdb at SeqGenesis (Daejeon, Korea), respectively, as previously described (Lim et al. 2015). As expected, 239 and 10 contigs related to Bean common mosaic virus and Cucumber mosaic virus, which have been previously reported in adzuki bean, were obtained from the adzuki bean transcriptome, respectively. However, in addition to this, 143 and 8 contigs were attributed to two soybean viruses, Soybean mosaic virus (SMV) and Soybean yellow mottle mosaic virus (SYMMV), respectively. To confirm the presen...

First Complete Genome Sequence of Barley Virus G Identified from Proso Millet (Panicum miliaceum) in South Korea

ABSTRACT The complete genome sequence of an Uiseong isolate of barley virus G (BVG) on proso millet plants in a field in South Korea was determined by RNA sequencing and Sanger sequencing. To our knowledge, this is the first complete genome sequence report of BVG infecting proso millet in South Korea.