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Dive into the research topics where Chunjian Zhao is active.

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Featured researches published by Chunjian Zhao.


Environmental Toxicology and Pharmacology | 2011

Chemical composition and antimicrobial activity of the essential oil of Rosemary

Yang Jiang; Nan Wu; Yujie Fu; Wei Wang; Meng Luo; Chunjian Zhao; Yuangang Zu; Xiao-Lei Liu

The composition of the essential oil of Rosemary was analyzed by gas chromatography-mass spectrometry (GC-MS). 22 components, which constitute 97.41% of the oil, were identified. The major constituents were 1,8-Cineole (26.54%) and α-Pinene (20.14%). Minimum inhibitory concentrations (MICs), minimal bactericidal concentration (MBC) and time-kill dynamic processes against three Gram-positive bacteria (Staphylococcus epidermidis, Staphylococcus aureus and Bacillus subtilis), three Gram-negative bacteria (Proteus vulgaris, Pseudomonas aeruginosa and Escherichia coli) and two fungi (Candida albicans and Aspergillus niger) were determined for the oil, 1,8-Cineole and α-Pinene. The oil showed pronounced antibacterial and antifungal activity than 1,8-Cineole and α-Pinene against all of the tested microbes. Furthermore, the survival rates and morphological changes of S. aureus after treatment with different concentrations of the essential oil were assessed by flow cytometry (FCM) and atomic force microscopy (AFM).


Journal of Chromatography A | 2011

Ionic liquid-based microwave-assisted extraction of essential oil and biphenyl cyclooctene lignans from Schisandra chinensis Baill fruits.

Chunhui Ma; Tingting Liu; Lei Yang; Yuangang Zu; Xiaoqiang Chen; Lin Zhang; Ying Zhang; Chunjian Zhao

Ionic liquid-based microwave-assisted extraction (ILMAE) has been successfully applied in extracting essential oil and four kinds of biphenyl cyclooctene lignans from Schisandra chinensis Baill. 0.25 M 1-lauryl-3-methylimidazolium bromide ionic liquid is selected as solvent. The optimum parameters of dealing with 25.0 g sample are 385 W irradiation power, 40 min microwave extraction time and 1:12 solid-liquid ratio. The yields of essential oil and lignans are 12.12±0.37 ml/kg and 250.2±38.2 mg/kg under the optimum conditions. The composition of the essential oil extracted by hydro-distillation, steam-distillation and ILMAE is analyzed by GC-MS. With ILMAE method, the energy consumption time has not only been shortened to 40 min (hydro-distillation 3.0 h for extracting essential oil and reflux extraction 4.0 h for extracting lignans, respectively), but also the extraction efficiency has been improved (extraction of lignans and distillation of essential oil at the same time) and reduces the environmental pollution. S. chinensis materials treated by different methods are observed by scanning electronic microscopy. Micrographs provide more evidence to prove that ILMAE is a better and faster method. The experimental results also indicate that ILMAE is a simple and efficient technique for sample preparation.


Bioresource Technology | 2012

Biodiesel production from yellow horn (Xanthoceras sorbifolia Bunge.) seed oil using ion exchange resin as heterogeneous catalyst

Ji Li; Yujie Fu; Xue-Jin Qu; Wei Wang; Meng Luo; Chunjian Zhao; Yuangang Zu

In this study, biodiesel production from yellow horn (Xanthoceras sorbifolia Bunge.) seed oil using ion exchange resin as heterogeneous catalyst was investigated. After illustration of the mechanisms of transesterification reactions catalyzed by typical ion exchange resins, the factors affecting microwave-assisted transesterification process were studied. A high conversion yield of about 96% was achieved under optimal conditions using high alkaline anion exchange resins as catalyst. Analyzing the FAMEs composition by GC-MS and main physical-chemical properties demonstrated that the biodiesel product prepared from yellow horn seed oil was of high quality. Compared with conventional alkali catalyst, the outstanding characteristics of reusability and operational stability made the resin catalyst more predominant for biodiesel production. In addition, a comprehensive kinetic model was established for analyzing the reaction. The results of present research showed that microwave-assisted transesterification process catalyzed by high alkaline anion exchange resin was a green, effective and economic technology for biodiesel industry.


Journal of Agricultural and Food Chemistry | 2013

Dihydroquercetin (DHQ) induced HO-1 and NQO1 expression against oxidative stress through the Nrf2-dependent antioxidant pathway.

Lu Liang; Chang Gao; Meng Luo; Wei Wang; Chunjian Zhao; Yuangang Zu; Thomas Efferth; Yujie Fu

Dihydroquercetin (DHQ) is a well-known antioxidant agent. In the present investigation, we reported for the first time that DHQ stimulates the expression of phase II detoxifying enzymes through the Nrf2-dependent signaling pathway. The IC50 values of DHQ for reduction of 2,2-diphenyl-1-picrylhydrazol (DPPH), reducing power assay, lipid peroxidation assay, and xanthine oxidase inhibition were 5.96, 4.31, 2.03, and 13.24 μM, respectively. DHQ possessed considerable protective activity from oxidative DNA damage. A luciferase reporter assay also demonstrated that DHQ-activated signaling resulted in the increased transcriptional activity of Nrf2 through binding to the ARE (antioxidant response element) enhancer sequence. Furthermore, Western blotting and luciferase assay revealed DHQ activated ERK1/2, Akt, and JNK signaling pathways, subsequently leading to Nrf2 nuclear translocation. DHQ upregulated the Nrf2-related antioxidant genes heme oxygenase-1 (HO-1), NAD(P)H quinone oxidoreductase-1 (NQO1), and glutamate-cysteine ligase modifier subunits. Inhibition of Nrf2 by siRNA reduced DHQ-induced upregulation of these antioxidant genes.


Food Chemistry | 2012

Development of an ionic liquid-based microwave-assisted method for simultaneous extraction and distillation for determination of proanthocyanidins and essential oil in Cortex cinnamomi.

Ye Liu; Lei Yang; Yuangang Zu; Chunjian Zhao; Lin Zhang; Ying Zhang; Zhonghua Zhang; Wenjie Wang

Cortex cinnamomi is associated with many health benefits and is used in the food and pharmaceutical industries. In this study, an efficient ionic liquid-based microwave-assisted simultaneous extraction and distillation (ILMSED) technique was used to extract cassia oil and proanthocyanidins from Cortex cinnamomi; these were quantified by gas chromatography/mass spectrometry (GC-MS) and the vanillin-HCl colorimetric method, respectively. 0.5M 1-butyl-3-methylimidazolium bromide ionic liquid was selected as solvent. The optimum parameters of dealing with 20.0 g sample were 230 W microwave irradiation power, 15 min microwave extraction time and 10 liquid-solid ratio. The yields of essential oil and proanthocyanidins were 1.24 ± 0.04% and 4.58 ± 0.21% under the optimum conditions. The composition of the essential oil was analysed by GC-MS. Using the ILMSED method, the energy consumption was reduced and the extraction yields were improved. The proposed method was validated using stability, repeatability, and recovery experiments. The results indicated that the developed ILMSED method provided a good alternative for the extraction of both the essential oil and proanthocyanidins from Cortex cinnamomi.


Journal of Pharmaceutical and Biomedical Analysis | 2012

PREPARATIVE SEPARATION OF DRYOFRAGIN AND ASPIDIN BB FROM DRYOPTERIS FRAGRANS EXTRACTS BY MACROPOROUS RESIN COLUMN CHROMATOGRAPHY

Xiao-Juan Li; Yujie Fu; Meng Luo; Wei Wang; Lin Zhang; Chunjian Zhao; Yuangang Zu

A simple, efficient and environment-friendly chromatographic separation method was developed for preparative separation and enrichment of dryofragin and aspidin BB from Dryopteris fragrans. The adsorption properties of twelve macroporous adsorption resins were evaluated. The three selected resins were further screened depending on the separation performance of their packed columns, in which AB-8 resin showed better separation efficiency for dryofragin and aspidin BB. In order to maximize column efficiency, the operating parameters (flow rate, ethanol concentration and volume) of the resin column chromatography were optimized and compared with the conventional resin column adsorption. After preparative separation and enrichment on resin column chromatography, the contents of dryofragin and aspidin BB in the product were 8.39- and 5.99-fold increased with recovery yields of 91.22% and 75.64%, respectively. Moreover, the regenerated adsorbent exhibited excellent reusability within at least five cycles of adsorption/desorption. It suggested that multi-targets would be enriched effectively by resin column chromatography.


Bioresource Technology | 2013

Oil removal from water with yellow horn shell residues treated by ionic liquid.

Ji Li; Meng Luo; Chunjian Zhao; Chunying Li; Wei Wang; Yuangang Zu; Yujie Fu

In the present study, yellow horn shell residues treated by ionic liquid (IL) were prepared for oil removal from water. Scanning electron microscope (SEM) analysis was applied to represent the effects of IL-treatment on this new cellulosic biosorbent. Furthermore, the comprehensive sorption capacities of the shell residues for five types of oils in pure oil and oily water medium were evaluated. The maximum sorption capacities of IL-treated shell residues (0.39-0.61 g/g) were about 1.5-fold to those of untreated shell residues (0.32-0.42 g/g), respectively. The effects of main environmental factors (temperature and pH) on oil removal were also studied. Kinetic models were successfully established for analyzing the oil sorption process. In addition, it was found that the IL-treated shell residues could be reused for several times. The results indicated that IL-treated yellow horn shell residues could be developed as an ideal biosorbent for oil removal from water.


Food Chemistry | 2013

Aqueous enzymatic process assisted by microwave extraction of oil from yellow horn (Xanthoceras sorbifolia Bunge.) seed kernels and its quality evaluation.

Ji Li; Yuangang Zu; Meng Luo; Cheng-Bo Gu; Chunjian Zhao; Thomas Efferth; Yujie Fu

In this study, aqueous enzymatic process (AEP) assisted by microwave extraction (ME) of oil from yellow horn (Xanthoceras sorbifolia Bunge.) seed kernel was investigated. Central composite design (CCD) and response surface methodology (RSM) were used to optimise an enzyme cocktail (cellulase, hemicellulase, pectinase) for AEP. The main factors of ME were also studied. A maximal oil extraction yield of 55.8% was achieved under optimal conditions. Moreover, scanning electron microscope (SEM) was applied to characterise the extraction process. Analysing chemical composition of the extracted oil by GC-MS showed that the content of unsaturated fatty acids by this emerging method (91.18%) was similar to that by conventional organic solvent extraction (88.76%). In addition, the main physicochemical properties and antioxidant activities of yellow horn oil were measured to evaluate its quality. The present research supported necessary data for the green extraction method of edible oil in food industry.


International Journal of Pharmaceutics | 2012

Preparation, characterization and in vivo assessment of the bioavailability of glycyrrhizic acid microparticles by supercritical anti-solvent process.

Xiaoyu Sui; Wei Wei; Lei Yang; Yuangang Zu; Chunjian Zhao; Lin Zhang; Fengjian Yang; Zhonghua Zhang

In this study, glycyrrhizic acid (GA) microparticles were successfully prepared using a supercritical anti-solvent (SAS) process. Carbon dioxide and ethanol were used as the anti-solvent and solvent, respectively. The influences of several process parameters on the mean particle size (MPS), particle size distribution (PSD) and total yield were investigated. Processed particle sizes gradually decreased as temperature and solution flow rate increased. In addition, processed particle sizes increased from 119 to 205 nm as GA concentration increased. However, CO(2) flow rate did not significantly affect particle size. The optimized process conditions were applied, those included temperature (65 °C), pressure (250 bar), CO(2) and drug solution flow rate (15 and 8 mL min(-1)), drug concentration in ethanol (20 mg mL(-1)). Microparticles with a span of PSD ranging from 95 and 174 nm, MPS of 128 nm were obtained, and total yield was 63.5%. The X-ray diffraction patterns of glycyrrhizic acid microparticles show apparent amorphous nature. Fourier transform infrared (FT-IR) spectroscopy results show that no chemical structural changes occurred. The in vitro dissolution tests showed that the GA microparticles exhibited great enhancement of dissolution performance when compared to GA original drug. Furthermore, the in vivo studies revealed that the microparticles provided improved pharmacokinetic parameter after oral administration to rats as compared with original drug.


International Journal of Molecular Sciences | 2012

Ultrasound-Assisted Extraction of Carnosic Acid and Rosmarinic Acid Using Ionic Liquid Solution from Rosmarinus officinalis

Ge Zu; Rongrui Zhang; Lei Yang; Chunhui Ma; Yuangang Zu; Wenjie Wang; Chunjian Zhao

Ionic liquid based, ultrasound-assisted extraction was successfully applied to the extraction of phenolcarboxylic acids, carnosic acid and rosmarinic acid, from Rosmarinus officinalis. Eight ionic liquids, with different cations and anions, were investigated in this work and [C8mim]Br was selected as the optimal solvent. Ultrasound extraction parameters, including soaking time, solid–liquid ratio, ultrasound power and time, and the number of extraction cycles, were discussed by single factor experiments and the main influence factors were optimized by response surface methodology. The proposed approach was demonstrated as having higher efficiency, shorter extraction time and as a new alternative for the extraction of carnosic acid and rosmarinic acid from R. officinalis compared with traditional reference extraction methods. Ionic liquids are considered to be green solvents, in the ultrasound-assisted extraction of key chemicals from medicinal plants, and show great potential.

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Yuangang Zu

Northeast Forestry University

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Yujie Fu

Northeast Forestry University

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Lei Yang

Northeast Forestry University

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Meng Luo

Northeast Forestry University

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Wei Wang

Northeast Forestry University

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Lin Zhang

Northeast Forestry University

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Chunying Li

Northeast Forestry University

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Fengjian Yang

Northeast Forestry University

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Baishi Zu

Northeast Forestry University

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Cheng-Bo Gu

Northeast Forestry University

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