Chyi-How Lay
Feng Chia University
Network
Latest external collaboration on country level. Dive into details by clicking on the dots.
Publication
Featured researches published by Chyi-How Lay.
International Journal of Hydrogen Energy | 2004
Chiu-Yue Lin; Chyi-How Lay
Abstract Anaerobic sewage sludge acclimated with sucrose was used as the seed in a batch experiment to investigate the carbon/nitrogen (C/N)-ratio effects on biological hydrogen production from sucrose. Experimental results indicated that the hydrogen production ability of the anaerobic microflora (dominated by Clostridium pasteurianum) in the sewage sludge was dependent on the influent C/N-ratio. At a C/N-ratio of 47, the hydrogen productivity and hydrogen production rate reached 4.8 mol-H 2 / mol-sucrose and 270 mmol-H 2 / L-day , respectively. This increased by 500% and 80%, respectively, compared with the blank. Proper C/N-ratio on hydrogen production enhancement was accomplished by shifting the metabolic pathway. Strategies for optimal hydrogen production are also proposed.
Biotechnology and Bioengineering | 2008
Perttu E.P. Koskinen; Chyi-How Lay; Jaakko A. Puhakka; Ping Jei Lin; Shu-Yii Wu; Johann Orlygsson; Chiu-Yue Lin
Dark fermentative hydrogen production from glucose by a thermophilic culture (33HL), enriched from an Icelandic hot spring sediment sample, was studied in two continuous‐flow, completely stirred tank reactors (CSTR1, CSTR2) and in one semi‐continuous, anaerobic sequencing batch reactor (ASBR) at 58°C. The 33HL produced H2 yield (HY) of up to 3.2 mol‐H2/mol‐glucose along with acetate in batch assay. In the CSTR1 with 33HL inoculum, H2 production was unstable. In the ASBR, maintained with 33HL, the H2 production enhanced after the addition of 6 mg/L of FeSO4 · 7H2O resulting in HY up to 2.51 mol‐H2/mol‐glucose (H2 production rate (HPR) of 7.85 mmol/h/L). The H2 production increase was associated with an increase in butyrate production. In the CSTR2, with ASBR inoculum and FeSO4 supplementation, stable, high‐rate H2 production was obtained with HPR up to 45.8 mmol/h/L (1.1 L/h/L) and HY of 1.54 mol‐H2/mol‐glucose. The 33HL batch enrichment was dominated by bacterial strains closely affiliated with Thermobrachium celere (99.8–100%). T. celere affiliated strains, however, did not thrive in the three open system bioreactors. Instead, Thermoanaerobacterium aotearoense (98.5–99.6%) affiliated strains, producing H2 along with butyrate and acetate, dominated the reactor cultures. This culture had higher H2 production efficiency (HY and specific HPR) than reported for mesophilic mixed cultures. Further, the thermophilic culture readily formed granules in CSTR and ASBR systems. In summary, the thermophilic culture as characterized by high H2 production efficiency and ready granulation is considered very promising for H2 fermentation from carbohydrates. Biotechnol. Bioeng. 2008;101: 665–678.
Bioresource Technology | 2013
Chyi-How Lay; Biswarup Sen; Chin-Chao Chen; Jou-Hsien Wu; Shih-Chi Lee; Chiu-Yue Lin
Hydrogen (H2) production potential of water hyacinth (WH) and beverage wastewater (BW) mixture in powder and pellet form at various combination ratios were evaluated. Batch co-fermentation results showed peak biogas production of 105.5 mL and H2 production of 55.6 mL at the combination ratio of 1.6 g WH and 2.4 g BW in pellet form. With the same ratio in pellet form, the maximum H2 production rate 542 mL H2/L-d, maximum specific H2 production rate 869 mL H2/g VSS-d and H2 yield 13.65 mL/g feedstock were obtained, and were 88, 88 and 34% higher than its powder form. The predominant soluble metabolite was acetate in the concentration of 1059-2639 mg COD/L (40-79% of total metabolites) in most runs during co-fermentation of mixed feedstock. Carbon-to-nitrogen ratio and the physical form of the combined feedstock are essential criteria for optimum H2 production. Co-fermentation also alleviates the waste disposal problem of the industries.
Reviews in Environmental Science and Bio\/technology | 2015
Estela Tapia-Venegas; Juan Esteban Ramirez-Morales; Fernando Silva-Illanes; Javiera Toledo-Alarcón; Florian Paillet; Renaud Escudié; Chyi-How Lay; Chen-Yeon Chu; Hoang-Jyh Leu; Antonella Marone; Chiu-Yue Lin; Dong-Hoon Kim; Eric Trably; Gonzalo Ruiz-Filippi
Currently, the use of alternative renewable energies is broadly supported in many countries, some of which are seriously evaluating the possibility of using hydrogen as an alternative fuel in their power systems. Hydrogen production by biological processes, such as dark fermentation, is a very promising alternative. However, this process has only been studied on the laboratory scale, and there is limited experience at the pilot scale. The main reasons of non-scaling hydrogen production by dark fermentation at large scale are unpurified hydrogen production, stability of the bioprocesses, as well as their low conversion yields joined at the formation of byproducts. Improvement of energetic yields of dark fermentation requires a better knowledge of the microorganisms involved in the mixed culture and their possible interactions, as well as the use of appropriate substrates and strategies, such as solid-state fermentation, the purification of hydrogen and the coupling of dark fermentation with other biological processes as anaerobic digestion. The present work offers an overview of the current knowledge dealing with H2-production by dark fermentation and its integration into a concept of an environmental biorefinery. Several key points are addressed, such as the benefits of using local waste as substrates, the new solid-state fermentation processes, the coupling of hydrogen purification with the production process, the association of the H2-producing process with other biological processes, such as anaerobic digestion towards biohythane production (H2/CH4). Information about pilot plant experiments was added to illustrate the feasibility of producing fermentative hydrogen and methane from organic waste at a pilot scale, as developed at Feng Chia University (Taiwan).
Biotechnology and Bioengineering | 2018
Jui-Jen Chang; Yu-Ju Lin; Chyi-How Lay; Caroline Thia; Yueh-Chin Wu; Yu-Han Hou; Chieh-Chen Huang; Wen-Hsiung Li
Cellulose is a renewable feedstock for green industry. It is therefore important to develop a technique to construct a host with a high cellulolytic efficiency to digest cellulose. In this study, we developed a convenient host‐engineering technique to adjust the expression levels of heterologous genes in the host by promoter rearrangement and gene copy number adjustment. Using genes from different glycoside hydrolase (GH) families including GH2, GH3, GH5, GH6, GH7, and GH12 from Aspergillus niger, Trichoderma reesei, and Neocallimastix patriciarum, we constructed a cellulolytic Kluyveromyces marxianus with eight cellulase gene‐cassettes that produced a cellulase cocktail with a high cellulolytic efficiency, leading to a significant reduction in enzyme cost in a rice straw saccharification process. Our technique can be used to design a host that can efficiently convert biomass feedstock to biofuel.
Current Developments in Biotechnology and Bioengineering#R##N#Biological Treatment of Industrial Effluents | 2017
Chiu-Yue Lin; Chyi-How Lay; Chin-Chao Chen
Various wastewaters rich in organic content are attractive for bioenergy production because of advantages such as high organic loading possibilities, low nutrient requirements, and positive net energy gain and are known as the second-generation biofuel feedstock. For bioenergy generation, the characteristics of food industrial wastewater, livestock industrial wastewater, and municipal wastewater sludge are described in this chapter. Examples of two basic bioreactor configurations of suspended-growth and granulation systems that are generally used for achieving anaerobic biomass immobilization and sustaining the desired efficient biochemical reactions are given. For efficient anaerobic treatment, some operation values of key parameters such as feedstock pretreatment, loading rate, pH, and temperature are summarized. Moreover, to show the potential of bioenergy production from wastewater, a net energy gain analysis is illustrated for vinasse, glycerin, and domestic wastewaters. As future prospects, the concept of a bioenergy and bioresources center for wastewater management, potential locations for constructing organic wastewater-based bioenergy production systems, and a biohydrogen utopia are proposed.
International Journal of Hydrogen Energy | 2005
Chiu-Yue Lin; Chyi-How Lay
International Journal of Hydrogen Energy | 2004
Chian-Yu Lin; Chyi-How Lay
International Journal of Hydrogen Energy | 2012
Chiu-Yue Lin; Chyi-How Lay; Biswarup Sen; Chen-Yeon Chu; Gopalakrishnan Kumar; Chin-Chao Chen; Jo Shu Chang
International Journal of Hydrogen Energy | 2010
Chyi-How Lay; Jou-Hsien Wu; Chin-Lang Hsiao; Jui-Jen Chang; Chin-Chao Chen; Chiu-Yue Lin