Clarence M. Lee

Trichinella spiralis: immunization of rats with an antigen fraction enriched for allergenicity.

Trichinella spiralis whole muscle larval extract was fractionated by gel filtration and anion-exchange chromatography, and the protein fractions were assayed for allergenicity by a footpad-swelling test in mice; IgE antibody levels in rats immunized with the fractions were determined by passive cutaneous anaphylaxis test in rats. By these methods, an allergenic fraction from T. spiralis was isolated. The fraction, F1-b, was shown to be monodisperse by analysis with SDS-PAGE, IEP, and isoelectric focusing, indicating that it is a single protein moiety with a molecular weight of approximately 45,000 and a pI of approximately 5.1. The Schiff-periodate test showed F1-b to be a glycoprotein. Rats immunized with F1-b had significantly fewer intestinal worms than did nonimmunized controls at 24 hr and 7 days after oral challenge with T. spiralis larvae.

Trypanosoma lewisi. Body weight gains and food consumption of riboflavin-deficient rats given living cells, cell homogenates and cell metabolic products

Abstract 1. 1. Riboflavin deficiency was studied in rats infected with Trypanosoma lewisi . 2. 2. Body weight gains in rats on complete, riboflavin-deficient or pair-fed control diets and given living cells or homogenate of T. lewisi showed significant increases over uninoculated controls. 3. 3. Irrespective of dietary group, animals receiving living cells or homogenate of T. lewisi ate more food than the control animals beginning 12 days after inoculation. 4. 4. Regardless of diet, no differences in weight gain or food consumption were seen in animals inoculated with physiological saline or metabolic products of T. lewisi when compared with uninoculated controls.

Effect of malnutrition on susceptibility of mice to Trypanosoma musculi: Vitamin A-deficiency

SummaryVitamin A-deficiency was studied in mice infected with Trypanosoma musculi.Irrespective of diet, trypomastigotes (trypanosomes) appeared in peripheral tail blood of all inoculated mice after 6-day incubation periods. On the average, vitamin A-deficient mice had parasitemias about 10 times greater than animals fed a complete diet and 8 times pair-fed controls. Parasitemias lasted longer in vitamin-deficient hosts, and reached a maximum five days later than those from control hosts.The action of the antibody which inhibits reproduction of the trypomastigotes was delayed five days in vitamin-deficient mice; in pair-fed animals ablastic action occurred slightly earlier than in normal control animals. The action of the terminal lytic antibody was delayed by 6 days in metabolically deficient mice than in normal controls and by 4 days in pair-fed controls.Body weight gains in mice on complete, vitamin A-deficient or pair-fed control diets and given living cells or homogenate of T. musculi showed significant increases over uninoculated controls. Irrespective of dietary group, animals receiving living cells or homogenate of T. musculi ate more food than the control animals beginning 10 days after inoculation. Regardless of diet, no differences in weight gain or food consumption were seen in animals inoculated with physiological saline or metabolic products of T. musculi when compared with uninoculated controls.

Trypanosoma duttoni: Oxygen uptake by liver slices of normal and pantothenate-deficient mice

SummaryEndogenously liver slices from mice infected with Trypanosoma duttoni and fed a control diet, consumed an average of 31 μl O2/mg N/hour which was significantly different from that (20 μl) utilized by uninfected mouse liver slices. Average endogenous n

Ultrastructure of Trypanosoma duttoni

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Trypanosoma duttoni: Body weight gains, food consumption, and longevity of pantothenate-deficient mice given living cells, cell homogenates, and cell metabolic products

nvalues for liver slices from pantothenate-deficient mice were 25 μl for infected and 20 μl for non-infected mice. Average endogenous n

Oxygen uptake by Trypanosoma lewisi—Complex cells. IV. “E” and “Sp” isolates☆

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