Clarice Weiss Arns
State University of Campinas
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Featured researches published by Clarice Weiss Arns.
Avian Diseases | 2010
Paulo A. N. Felippe; L. H. A. da Silva; Maciel Santos; Fernando Rosado Spilki; Clarice Weiss Arns
Abstract To detect the presence of infectious bronchitis virus or avian coronavirus, a nested reverse transcriptase PCR (RT-PCR) method was developed with the aim of amplifying a fragment of 530 bases, comprising the gene coding S1 protein. In the first step, all samples were submitted to RNA extraction, RT-PCR, and nested PCR. Next, only the positive nested-PCR samples were propagated in specific-pathogen-free (SPF) embryonated chicken eggs for virus isolation. Positive samples were then sequenced and analyzed using a molecular phylogeny approach. Tracheal swab samples were collected from 23 different domestic chickens distributed in three regions of Brazil, in the period between 2003 and 2009. Also analyzed were six swab samples (tracheal and cloacal) from asymptomatic pigeons (Columba livia), caught in an urbanized region in southeastern Brazil. The study revealed two major phylogenetic groups: one clustered with the Massachusetts vaccine serotype and another joined with the D207 strain. Interestingly, samples grouped with the Connecticut and Arkansas serotypes were also found. Pigeon isolates clustered with the Massachusetts serotype showed significant similarity (close to 100%) to those obtained from chickens. Only one pigeon isolate was seen to be grouped with the Connecticut serotype, and no correlation was observed between sample grouping and region origin. Understanding the diversity of genotypes and eco-epizootiology of the disease in different environments is expected to be helpful for vaccine production aimed at the main circulating variants. In this respect, one could also expect benefits in the management of other bird species that may act as avian coronavirus reservoirs.
Brazilian Journal of Poultry Science | 2009
Maria Angela Orsi; L. Doretto Júnior; D. Reischak; Lha da Silva; Fernando Rosado Spilki; Maria da Glória Buzinaro; Clarice Weiss Arns
Intracerebral pathogenicity index (ICPI) and mean death time (MDT) were determined using commercial live vaccines against Newcastle disease available in Brazil. The ICPI profiles obtained for B1 vaccine strains were nonvirulent and varied from 0 to 0.19, and their MDT was 104-116 hours. The LaSota strains had an ICPI varying between 0.02 and 0.37 and MDT from 92 to 116 hours. ICPI and MDT for the Clone 30 were 0.11 and 104 hours, respectively. For Ulster vaccines, ICPI and MDT were 0 and >150 hours; for VG-GA was 0.03 and 140 hours; and for C2, 0.04 and >144 hours. Eye drop vaccination and IM challenge, at the 1 st week and the 4 th week, respectively, resulted in highest protection for B1 (95-100%) and LaSota (90-100%) strains. The variability in vaccine ICPI did not interfere with immune response and all vaccines provided similar protection. All vaccines were considered non virulent and were classified as lentogenic according to the immunobiological product standards.
Brazilian Journal of Poultry Science | 2015
Luciana K. Kohn; Mary Ann Foglio; Rodney Alexandre Ferreira Rodrigues; Im de Oliveira Sousa; Matheus C. Martini; Ma Padilla; Df de Lima Neto; Clarice Weiss Arns
Avian metapneumovirus (aMPV) is a negative-sense single-stranded RNA enveloped virus of the Metapneumovirus genus belonging to theParamyxoviridae family. This virus may cause significant economic losses to the poultry industry, despite vaccination, which is the main tool for controlling and preventing aMPV. The aim of this study was to evaluate the antiviral activity of extracts of four different native plants of the Brazilian Cerrado against aMPV. The antiviral activity against aMPV was determined by titration. This technique measures the ability of plant extract dilutions (25 to 2.5 µg mL-1) to inhibit the cytopathic effect (CPE) of the virus, expressed as inhibition percentage (IP). The maximum nontoxic concentration (MNTC) of the extracts used in antiviral assay was 25 µg mL-1for Aspidosperma tomentosumand Gaylussacia brasiliensis, and 2.5 µg mL-1for Arrabidaea chicaand Virola sebifera. Twelve different extracts derived from four plant species collected from the Brazilian Cerrado were screened for antiviral activity against aMPV. G. brasiliensis, A. chica,and V. sebifera extracts presented inhibition rates of 99% in the early viral replication stages, suggesting that these extracts act during the adsorption phase. On the other hand, A. tomentosum inhibited 99% virus replication after the virus entered the cell. The biomonitored fractioning of extracts active against aMPV may be a tool to identify the active compounds of plant extracts and to determine their precise mode of action.
Arquivo Brasileiro De Medicina Veterinaria E Zootecnia | 2011
Luciana Helena Antoniassi da Silva; K. C. Cardoso; M. J. Silva; Fernando Rosado Spilki; Clarice Weiss Arns
The aim of this work was the cloning of those transmembrane glycoproteins G and F from an isolate bovine respiratory syncytial viruses (BRSV) - a Brazilian isolate of BRSV, named BRSV-25-BR in previous studies, in a prokaryotic system to proceed the sequencing of larger genomic fragments. The nucleotide substitutions were confirmed and these clones may also be used in further studies regarding the biological effects of those proteins in vitro and in vivo.
VII. International symposium on avian corona- and pneumoviruses and complicating pathogens, Rauischholzhausen, Germany, 18-21 June 2012. | 2012
Paulo A. N. Felippe; Maurício B. dos Santos; L. H. A. da Silva; Sonia Tatsumi Sakata; Matheus C. Martini; Humberto Ferreira; Clarice Weiss Arns; M. Lierz; U. Heffels-Redmann; E. F. Kaleta; J. Heckmann
Archive | 2012
Gutierrez Nelia Rosa; H. G. Domingues; Márcia Mercês Aparecida Bianchi dos Santos; Paulo A. N. Felippe; Fernando Rosado Spilki; Clarice Weiss Arns
Archive | 2011
H. G. Domingues; Fernando Rosado Spilki; Clarice Weiss Arns
Archive | 2011
Adriana Gut Lopes Riccetto; Elizabeth Belfiore; M. K. Pupim; Lenice Heloisa de Arruda Silva; Fernando Rosado Spilki; Clarice Weiss Arns; M. T. N. Silva; Emílio Carlos Elias Baracat
Archive | 2011
H. G. Domingues; Fernando Rosado Spilki; Clarice Weiss Arns
Archive | 2011
Lha Da Silva; Karina Cordeiro Cardoso; M.A.A. da Silva; Fernando Rosado Spilki; Clarice Weiss Arns