Claude Périssol

Phenoloxidases of the white-rot fungus Marasmius quercophilus isolated from an evergreen oak litter (Quercus ilex L.)

Abstract A white-rot basidiomycete, Marasmius quercophilus , was isolated from an evergreen oak litter. This fungus, which very thoroughly bleaches the whole leaf area, produces laccases, a property which has not been studied before. Except on a ground leaf medium, we did not reveal phenoloxidases other than laccases. M. quercophilus is able to transform many aromatic compounds which are by-products of lignin and tannin metabolism. We observed, for example, that lignin monomers, namely p -coumaryl, coniferyl, and sinapyl alcohols are oxidized at different rates by the extracellular crude enzyme extract. The highest production of laccases is obtained with shaking cultures on malt extract supplemented with Tween 80 (0.1%) and CuSO 4 (0.5 mg l −1 ). These enzymes have an optimum activity at 80°C and a stable activity at 40°C for 18 h. The extracellular crude enzyme extract medium contains three isoenzymes with pI values of 3.8, 3.5, and 3.2. It is able to significantly reduce the kappa number (21.2%) of a kraft pulp without inclusion of any mediator.

Comparison between UV spectroscopy and Nirs to assess humification process during sewage sludge and green wastes co-composting.

The humification of organic matter during composting was studied by the quantification and monitoring of the evolution of humic substances (Humic Acid-HA and Fulvic Acid-FA) by UV spectra deconvolution (UVSD) and near-infrared reflectance spectroscopy (NIRS) methods. The final aim of this work was to compare UVSD to NIRS method, already applied on the same compost samples in previous studies. Finally, UVSD predictions were good for HA and HA/FA (r(2) of 0.828 and 0.531) but very bad for FA (r(2) of 0.092). In contrary, all NIRS correlations were accurate and significant with r(2) of 0.817, 0.806 and 0.864 for HA, FA and HA/FA ratio respectively. From these results, HA/FA ratio being a well-used index of compost maturity, UVSD and NIRS represent two invaluable tools for the monitoring of the composting process. However, we can note that NIRS predictions were more accurate than UVSD calibrations.

Influence of plants on microbial activity in a vertical-downflow wetland system treating waste activated sludge with high organic matter concentrations

The rhizosphere is a key zone for pollutant removal in treatment wetlands; therefore, studies on microbial activity may provide helpful information for a better understanding of purification processes. We studied microbial activity in a vertical-downflow constructed wetland system treating waste activated sludge with high organic matter concentrations, under Mediterranean climate. The aims of the work were to study the influence of (i) the presence of plants, (ii) the plant species (Phragmites australis Cav., Typha latifolia L., Iris pseudacorus L.), and (iii) the plant growth stage (plant senescence and plant fast growing stage) on total respiration rate and phosphatase activity in the substrate (intented here as the solid support on which the plants grow). The presence of plants had a positive influence on microbial activity, since substrate respiration and both acid and alkaline phosphatase activity were always higher in planted than in unplanted mesocosms. Among the three tested species, Phragmites was the one that most stimulated both substrate respiration rate and phosphatase activity, followed by Typha and Iris. These differences of microbial activity between mesocosms were corresponding to differences of removal efficiency. Substrate respiration and phosphatase activity were of similar magnitude at the two growth stages, while the stimulating effect of plants seemed to have been delayed and microbial activity showed higher fluctuations at plant fast growing stage than at plant senescence.

Changes in the level of alkaline and acid phosphatase activities during green wastes and sewage sludge co-composting

The aim of this work was to study the activity level of alkaline and acid phosphatases during the composting of green wastes and sewage sludge and to determine relationships between biotic and abiotic properties of compost and phosphatase activities. This study has revealed a noticeable separation of phosphomonoesterase activities into two distinct phases during composting. Alkaline and acid phosphatase activities were high during the first month of composting and then declined up to the end of the process. Both phosphatase activities were significantly correlated with a group of variables which are clearly related to advancement of maturity during the composting process: C/N, respiration, cellulase, protease, phenoloxidase activities, HA and Global Index of Composting Evolution (GICE) resulting from 14 biological and chemical parameters. This study has also revealed the complexity of factors regulating turnover of P in compost process since phosphatase activities appeared constrained across two thresholds: 60/70 and 100 microg of inorganic P g(-1) DM for activation and repression, respectively.

Functional changes in culturable microbial communities during a co-composting process: carbon source utilization and co-metabolism.

Microbial communities in sewage sludge and green waste co-composting were investigated using culture-dependent methods and community level physiological profiles (CLPP) with Biolog Microplate. Different microbial groups characterized each stage of composting. Bacterial densities were high from beginning to end of composting, whereas actinomycete densities increased only after bio-oxidation phase i.e. after 40days. Fungal populations become particularly high during the last stage of decomposition. Cluster analyses of metabolic profiles revealed a similar separation between two groups of composts at 67days for bacteria and fungi. Principal component analysis (PCA) applied to bacterial and fungal CLPP data showed a chronological distribution of composts with two phases. The first one (before 67days), where the composts were characterized by the rapid decomposition of non-humic biodegradable organic matter, was significantly correlated to the decrease of C, C/N, organic matter (OM), fulvic acid (FA), respiration, cellulase, protease, phenoloxidase, alkaline and acid phosphatases activities. The second phase corresponding to the formation of polycondensed humic-like substances was significantly correlated to humic acid (HA) content, pH and HA/FA. The influent substrates selected on both factorial maps showed that microbial communities could adapt their metabolic capacities to the particular environment. The first phase seems to be focused on easily degradable substrate utilization whereas the maturation phase appears as multiple metabolisms, which induce the release of metabolites and their polymerization leading to humification processes.

Composting parameters and compost quality: a literature review

Economic growth and rising food consumption in the world have led to increased production of organic wastes due to an important intensification of the agricultural production systems. The analysis of macro elements (nitrogen [N], phosphorus [P], and potassium [K]) in organic wastes reveals that the economic loss is significant with the intensification of the agricultural system. The good management of those wastes would be an effective means of organic matter restoration through carbon restitution to the depleted soils through organic amendments. Composting is a type of waste processing that has gained increasing acceptance over the years. As a rule, the process consists of the natural biological decomposition of organic waste components and involves diverse species of microorganisms. Those organic residues could reconstitute soils and be an important fertilization backup. Composts prepared from different organic wastes differ in their quality and stability, which further depends upon the composition of raw material used for the compost production. Compost quality is closely related to its stability and maturity. The wide variety of chemical and biological variations that occur during composting, and the range of methods suggested in literature, has made it difficult to agree on methods for the practical assessment of maturity. A literature review of the main start-up, monitoring and maturity parameters are discussed concerning different raw materials used and different composting methods practiced.