Claudia Bernal

Porcine model for hybrid material of carbonated apatite and osteoprogenitor cells

Abstract Culture and characterisation of porcine osteoprogenitors were established in order to study the differentiation of osteoblast in a hybrid material of carbonated apatite (CAp) (patent under process no. PI 20042321) and autologous cells that can be used later as an advanced bone graft material. Cells were obtained from bone marrow and cultured onto CAp of wet synthesis and onto Thermanox, with or without differentiation factors in time course experiments, at the end of which biochemical and molecular markers of osteogenesis were obtained. Results indicated that this model is useful to study the functionality of a hybrid material of synthetic CAp and autologous osteoprogenitor cells derived from porcine bone marrow.