Cláudia Roberta Leite Vieira de Figueiredo

Titanium implants: A removal torque study in osteopenic rabbits

CONTEXT Osteoporosis is a skeletal disorder characterized by low bone mass and microarchitectural deterioration of bone tissue. It may affect the craniomaxillofacial complex and result in less bone-implant contact. AIMS The objective of this study was to measure the endosseous integration of titanium implants using the removal torque test in low-mineral density experimental animal models. MATERIALS AND METHODS Thirty female New Zealand white rabbits were divided into two groups: control (n=14), sham-operated; and test (n=16), ovariectomized. All animals received 2.2 mm Χ 4.0 mm titanium implants in the right tibia, 120 days after ovariectomy or sham surgery. Animals were killed at 7 and 14 weeks of postimplant insertion, when implants were removed by reverse torque rotation using a digital torque meter. Statistical analysis used was Students t-test. RESULTS AND CONCLUSION At the 7-week period, the mean torque values for the control and test groups were 11.6 Ncm and 10.4 Ncm, respectively, with no statistical significance. Implant removal torque analysis showed statistically lower values (P < 0.05) for the test group at the 14-week period (control=16.7 Ncm and test=9.7 Ncm). The results of this study indicate that hormonal privacy compromise implants biomechanical performance.

CD1a-positive Langerhans cells and their relationship with E-cadherin in ameloblastomas and keratocystic odontogenic tumors

BACKGROUND Ameloblastomas and keratocystic odontogenic tumors (KOTs) are lesions that are characterized by locally invasive growth and cause extensive bone destruction. In addition, it is known that E-cadherin influences the adhesion of Langerhans cells (LCs) to keratinocytes. OBJECTIVE AND METHODS The aim of this study was to investigate, using immunohistochemistry, the distribution of CD1a-positive cells in ameloblastomas and KOTs and their relationship with E-cadherin, in comparison to calcifying cystic odontogenic tumor (CCOT). RESULTS The CD1a-positive LCs were observed in 11 ameloblastomas and KOTs. All of the cases of CCOT showed CD1a-positive LCs and a significant difference was found when this tumor was compared with ameloblastomas (P < 0.05, Mann-Whitney test). A statistically significant difference was also noted when comparing CD1a-positive LCs between CCOTs and KOTs (P < 0.05, Mann-Whitney test). Lower expression of E-cadherin in ameloblastomas (AMs) in relation to KOTs and CCOTs (P < 0.05, Fisher test) was observed. There was no correlation between E-cadherin and CD1a-positive LCs between all odontogenic tumors that were studied (P > 0.05, Spearman test). CONCLUSION A quantitative difference of CD1a-positive cells between AMs and KOTs in comparison to CCOTs was observed. This permits to speculate that a depletion of CD1a-positive LCs might influence the local invasiveness of ameloblastomas and KOTs. Furthermore, it is suggested that E-cadherin mediates cell adhesion in these tumors.

Immunohistochemical evaluation of the inflammatory response in periodontal disease

In order to contribute to the knowledge of the pathogenesis of periodontal disease, an immunohistochemical analysis of the density of inflammatory mononucleated cells and the number of dendritic cells was performed using anti-CD4, anti-CD20, anti-CD25, anti-CD68 and anti-protein S-100 antibodies in 17 cases of chronic gingivitis (CG) and 25 of chronic periodontitis (CP). The CD4+ and CD68+ cells exhibited a diffuse distribution in the connective tissue. CD20+ cell distribution was predominantly in groups and the CD25+ cells exhibited a diffuse or focal distribution. The S-100+ cells were identified in the epithelium and the lamina propria, exhibiting distinct morphology and number. The statistical analysis showed no significant differences (p>0.05) between CG and CP regarding the density of the CD4+ and CD20+ cells and the number of S-100+ cells. However, significant differences (p<0.05) were found between the groups in the density of CD25+ and CD68+ cells . The density of macrophages was greater in CG and the level of cellular activation of the lymphocyte infiltrate was greater in CP. No differences were detected between the aforementioned conditions regarding the density of the T and B lymphocytes and to the number of the dendritic cells.

Queilite actínica: avaliação histopatológica de 44 casos

Objetivo:Descrever os aspectos epidemiologicos, clinicos e histopatologicos da queilite actinica.Material e metodo:Foi realizado um estudo retrospectivo a partir de casos com diagnostico clinico de queilite actinica, registrados no arquivo histopatologico do Servico de Cirurgia de Cabeca e Pescoco do Hospital Napoleao Laureano, Paraiba, relativos ao periodo de 2000 a 2007. Foram selecionados 44 blocos parafinados, que apresentavam condicoes de reavaliacao histologica atraves da confeccao de novas lâminas. Os novos cortes foram corados em Hematoxilina e Eosina, e a avaliacao histopatologica foi realizada por dois examinadores independentes, sendo as alteracoes classificadas de acordo com OMS. Realizou-se analise estatistica descritiva em programa SPSS for Windows versao 13.Resultado:Do total da amostra, 52,3% (23) dos casos foram diagnosticados em homens e 47,7% (21) em mulheres, com idade variando de 27 a 92 anos. A maioria dos individuos (81,9%) tinha mais de 40 anos. Em relacao as caracteristicas histologicas, 68,2% (30) dos casos exibiram algum grau de displasia epitelial, sendo 36,3% (16) classificados como displasia leve, 20,4% (9) como displasia moderada e 11,3% (5), displasia severa. Em 15,9% (7), ocorreu carcinoma de celulas escamosas. No tecido epitelial de revestimento labial, os achados histologicos mais frequentes e identificados foram presenca de degeneracao hidropica (79,5%) e hipergranulose (56,8%). Infiltrado inflamatorio foi observado em 88,6% dos casos e elastose solar, em 86,4%.Conclusao:Com base nos resultados da amostra estudada, podemos concluir que a maioria das lesoes de queilite actinica acometeu labio inferior de homens, com mais de 40 anos de idade. A analise histopatologica revelou displasia e atipia celular, infiltrado inflamatorio e presenca de elastose solar, que sao caracteristicas comuns em lesoes de QA.

Associação entre displasia e inflamação em queilite actínica

INTRODUCTION: Actinic cheilitis may undergo malignant transformation to squamous cell carcinoma. Furthermore, there is evidence that inflammatory components may be involved in this process, hence the importance of studies involving cellular atypia and chronic inflammation in this lesion. OBJECTIVE: The aim of this study was to evaluate the histological features of actinic cheilitis considering the degree of dysplasia, the intensity of inflammatory infiltrate and the possible correlation between the variables. MATERIAL AND METHODS: We selected 45 paraffin blocks of lesions with clinical and histopathological diagnosis of actinic cheilitis that enabled histopathological evaluation. The histopathological analysis was performed and the dysplasias and inflammatory infiltrates were classified into mild, moderate and severe. Data were analyzed through descriptive statistics, chi-square test and Analysis of variance (ANOVA), considering p < 0.05. RESULTS: 59.5% (27) of cases of actinic cheilitis showed some degree of dysplasia, from which 40% (18) presented mild dysplasia, 11.1% (5) moderate and 8.4% (4) severe. Squamous cell carcinoma occurred in 20% (9) of the specimens. All cases showed some degree of inflammation, from which 48.9% (22) showed mild inflammatory infiltrate, 20% (9) moderate and 31.1% (14) intense. There was a statistically significant association between the degree of dysplasia and the intensity of inflammatory infiltrate, namely ANOVA p = 0.000 and chi-square p = 0.004. CONCLUSIONS: The results indicate that the occurrence of dysplasia and squamous cell carcinoma are common in actinic cheilitis and the inflammatory process was closely related to the progression of epithelial atypia.

Pediatric fibromatosis involving mandible: case reportand a five-year post-operative follow-up

Fibromatose extra-abdominal e uma neoplasia fibrosa benigna, caracterizada por comportamento agressivo local. Geralmente, o tratamento de escolha consiste na excisao cirurgica com ampla margem de seguranca. O objetivo deste trabalho e relatar um caso de fibromatose em uma paciente de 11 anos, melanoderma, que apresenta aumento de volume na mandibula do lado direito. A partir do diagnostico histopatologico inicial sugestivo de lesao benigna de origem neural, foi realizado tratamento cirurgico conservador. Um novo exame anatomopatologico da peca cirurgica foi compativel com fibromatose. A paciente encontra-se sob controle periodico de cinco anos da cirurgia, nao havendo recorrencia clinica da lesao

Calcifying Epithelial Odontogenic Tumor

The calcifying epithelial odontogenic tumor (CEOT) is a rare benign epithelial odontogenic neoplasm of slow growth that is locally aggressive and tends to invade bone and adjacent soft tissue. Here is reported the case of a 21-year-old female patient with a CEOT in the left mandibular posterior region. The computerized tomography in coronal plane revealed a hypodense lesion in the posterior region of the left mandibular body with hyperdense areas inside and was associated with element 37. An incisional biopsy of the lesion was performed and the histopathological analysis revealed the presence of layers of epithelial odontogenic cells that formed prominent intercellular bridges. A large quantity of extracellular, eosinophilic, and amyloid-like material and an occasional formation of concentric calcifications (Liesegang rings) were also found. The histopathological diagnosis was a Pindborg tumor. Resection of the tumor with a safety margin was performed and after 6 months of follow-up there has been no sign of recurrence of the lesion.

Estudio comparativo del patrón de expresión inmunohistoquímico de las células CD4+ y CD20+ en la enfermedad periodontal

Resumen Antecedentes y metodos El papel de la respuesta inmune en la citopatogenia de la enfermedad periodontal constituye tema de estudio de diversos investigadores. El objetivo de este trabajo fue investigar a traves de inmunohistoquimica la densidad y el patron de distribucion de la celulas inflamatorias mononucleadas (linfocitos T helper e linfocitos B), utilizando los anticuerpos anti CD4 y anti CD20 en 17 casos de gingivitis cronica y 25 casos de periodontitis cronica. Resultados Fue observada inmunopositividad para las celulas CD4 en un patron difuso por todo tejido conectivo de todos los casos de gingivitis cronica. De los 25 casos de periodontitis cronica, 22 (88%) presentaron un patron de distribucion en pequenos focos aislados. Con relacion a las celulas CD20, 16 casos de gingivitis cronica exhibieron positividad en un patron focal de distribucion tanto en posicion yuxtaepitelial como en areas distantes del epitelio (10 casos, 62,5%). En los casos de periodontitis cronica fue observada marcacion positiva para el anticuerpo anti CD20 en 22 casos (88%), predominando el patron focal en posicion yuxtaepitelial y distante del epitelio (17 casos, 77,3%). El analisis estadistico no mostro diferencia significativa en la densidad de celulas CD4 y CD20 positivas entre los casos de gingivitis y periodontitis cronica. Conclusion Fue concluido que a pesar de no ser constatada tal diferencia estadistica, hubo una mayor densidad de linfocitos B en ambos grupos, sugiriendo una mayor participacion de la respuesta inmune humoral el los diferentes estadios de la enfermedad periodontal.