Clint D. McCullough

MICROCOSM TESTING OF MUNICIPAL SEWAGE AND GREEN WASTE FOR FULL-SCALE REMEDIATION OF AN ACID COAL PIT LAKE, IN SEMI-ARID TROPICAL AUSTRALIA

Pit lakes (abandoned flooded mine pits) represent a potentially valuable water resource to mining companies, the environment and regional communities across arid inland Australia. However, the water is often of low pH with high dissolved metal concentrations. The addition of organic matter to the pit lakes to enhance microbial sulfate reduction is potentially a cost effective and sustainable remediation strategy for these acid waters. However, the cost and availability of sufficient quantities of suitable organic substrates is typically limiting in these remote regions. Nevertheless, small quantities of sewage and green waste (organic garden waste) are often available in these areas from the regional towns which support the mines. This paper reports on preliminary microcosm laboratory experiments in preparation for the treatment of an acid (pH 2.2) coal mine pit lake in semi-arid tropical, inland north Queensland, Australia with municipal treated sewage and green waste. A laboratory experiment using microcosms (acrylic tubes) containing acid pit lake water and sediment were treated as follows; controls (untreated), sewage, green waste and sewage and green waste. The pH increased to a maximum of 5.5 in 145 days in the green waste and sewage treatment, with notable decreases of iron, aluminium and toxic heavy metals. Our results indicated that the green waste was a key component in alkalinity production and heavy metal removal.

Upper and Lower Concentration Thresholds for Bulk Organic Substrates in Bioremediation of Acid Mine Drainage

Acidic pit lakes may form in open cut mine voids that extend below the groundwater table and fill from surface and groundwater in-flows at the cessation of mining. Pit lake water quality may often be affected by acid mine drainage (AMD). Among the many remediation technologies available, sulphate reducing bacteria (SRB) based bioremediation using organic wastes appears to have significant potential towards ameliorating AMD effects of elevated acidity, metal and sulphate concentrations. A microcosm experiment was carried out under controlled conditions to assess the effect of different substrate concentrations of sewage sludge on AMD bioremediation efficiency. Experimental microcosms were made of 300 mm long and 100 mm wide acrylic cores, with a total volume of 1.8 L. Four different concentrations of sewage sludge (ranging 30–120 g/L) were tested. As the sewage sludge concentration increased the bioremediation efficiency also increased reflecting the higher organic carbon concentrations. Sewage sludge contributed alkaline materials that directly neutralised the AMD in proportion to the quantity added and therefore plays a primary role in stimulating SRB bioremediation. The lowest concentration of sewage sludge (30 g/L) tested proved to be inadequate for effective SRB bioremediation. However, there were no measurable beneficial effects on SRB bioremediation efficiency when sewage sludge was added at concentration >60 g/L.

IN-SITU COAL PIT LAKE TREATMENT OF ACIDITY WHEN SULFATE CONCENTRATIONS ARE LOW

Pit lakes (abandoned flooded mine pits) represent a potentially valuable resource to mining companies, the environment and community, if appropriate water quality can be achieved. However, the water is often of low pH with high dissolved metal concentrations. In Western Australia coal pit lakes are acidic (pH 3-5) but with low concentrations of sulfate and metals. Low sulfate concentrations prevent microbial sulfate reduction from reducing acidity in these lakes. However, stimulation of primary production and associated alkalinity generating processes may provide a cost effective and sustainable solution to the acidity problems. A field-scale experiment (with control) involving the treatment of in-situ macrocosms (~600 m 3 ) in a small south-west, Western Australian coal mine lake with municipal mulch and phosphorus additions to enhance primary production was undertaken between June 2003 and June 2004. One macrocosm was treated with P additions, another with mulch, a third with mulch and P, and the untreated lake formed the control. Physico-chemical and algal (chlorophyll a) sampling of the macrocosms and lake occurred at monthly intervals. The decomposition of mulch reduced nitrogen concentrations in the macrocosms to very low levels and necessitated supplementation with urea fertilizer. Phosphorus concentrations dropped rapidly after addition as it became bound to iron, organic matter and sediment. Although there was virtually no difference between treatments and control for most physico-chemical parameters measured (including pH), a PCA of the data showed that the addition of mulch sent the macrocosms on a different trajectory to the control. This difference was reflected in observations of increased abundance and diversity of biofilms and macroinvertebrates within the treated macrocosms. In conclusion, the addition of mulch and phosphorus alone was not sufficient to increase the pH of Collie mine lakes, although it does provide a number of benefits for biota in the water. We therefore recommend that liming be used to increase pH, followed by organic matter and nutrient additions to stimulate primary production.

Genotypic and morphological variation between Galaxiella nigrostriata (Galaxiidae) populations: Implications for conservation

Galaxiella nigrostriata is a freshwater fish that is endemic to the seasonally dry coastal wetlands of south-west Western Australia and considered by the International Union for Conservation of Nature (IUCN) as lower risk–near threatened. This small fish (maximum total length<50mm) aestivates in the sediment over the long, dry Mediterranean summer and its dispersal is limited by lack of habitat connectivity. The objective of this study was to identify the historical and contemporary genetic connectivity between populations of G. nigrostriata and to assess morphological variation between these populations. Results showed that all populations were genetically divergent and no mtDNA haplotypes were shared between populations. In contrast, morphological differentiation between individual populations was weak; however, pooling populations into two broad regions (Swan coastal plain and southern coast) resulted in clear morphological differentiation between these two groups. Based on these results, we postulate G. nigrostriata distribution last expanded in the early Pleistocene ~5.1 million years ago and have since been restricted to remnant wetlands in the immediate area. Galaxiella nigrostriata populations at the northern end of their range are small and are the most vulnerable to extinction. Conservation efforts are therefore required to ensure the survival of these genetically and morphologically distinctive Swan coastal plain populations.