Costel Flueraru

Graded-index fiber lens proposed for ultrasmall probes used in biomedical imaging

The quality and parameters of probing optical beams are extremely important in biomedical imaging systems both for image quality and light coupling efficiency considerations. For example, the shape, size, focal position, and focal range of such beams could have a great impact on the lateral resolution, penetration depth, and signal-to-noise ratio of the image in optical coherence tomography. We present a beam profile characterization of different variations of graded-index (GRIN) fiber lenses, which were recently proposed for biomedical imaging probes. Those GRIN lens modules are made of a single mode fiber and a GRIN fiber lens with or without a fiber spacer between them. We discuss theoretical analysis methods, fabrication techniques, and measured performance compared with theory.

3x3 Mach-Zehnder interferometer with unbalanced differential detection for full-range swept-source optical coherence tomography.

Quadrature interferometry based on 3x3 fiber couplers could be used to double the effective imaging depth in swept-source optical coherence tomography. This is due to its ability to suppress the complex conjugate artifact naturally. We present theoretical and experimental results for a 3x3 Mach-Zehnder interferometer using a new unbalanced differential optical detection method. The new interferometer provides simultaneous access to complementary phase components of the complex interferometric signal. No calculations by trigonometric relationships are needed. We demonstrate a complex conjugate artifact suppression of 27 dB obtained in swept-source optical coherence tomography using our unbalanced differential detection. We show that our unbalanced differential detection has increased the signal-to-noise ratio by at least 4 dB compared to the commonly used balanced detection technique. This is due to better utilization of optical power.

Texture analysis of optical coherence tomography speckle for characterizing biological tissues in vivo

We demonstrate a method for differentiating tissue disease states using the intrinsic texture properties of speckle in optical coherence tomography (OCT) images of normal and tumor tissues obtained in vivo. This approach fits a gamma distribution function to the nonlog-compressed OCT image intensities, thus allowing differentiation of normal and tumor tissues in an ME-180 human cervical cancer mouse xenograft model. Quantitative speckle intensity distribution analysis thus shows promise for identifying tissue pathologies, with potential for early cancer detection in vivo.

Simultaneous dual-wavelength-band common-path swept-source optical coherence tomography with single polygon mirror scanner.

We report a novel (to the best of our knowledge) simultaneous 1310/1550 two-wavelength band swept laser source and dual-band common-path swept-source optical coherence tomography (SS-OCT). Synchronized dual-wavelength tuning is performed by using two laser cavities and narrowband wavelength filters with a single dual-window polygonal scanner. Measured average output powers of 60 and 27 mW have been achieved for the 1310 and 1550 nm bands, respectively, while the two wavelengths were swept simultaneously from 1227 to 1387 nm for the 1310 nm band and from 1519 to 1581 nm for the 1550 nm band at an A-scan rate of 65 kHz. Broadband wavelength-division multiplexing is used for coupling two wavelengths into a common-path single-mode GRIN-lensed fiber probe to form dual-band common-path SS-OCT. Simultaneous OCT imaging at 1310 and 1550 nm is achieved. This technique allows for in vivo high-speed OCT imaging with potential application in functional (spectroscopic) investigations.

Waveguide shaping and writing in fused silica using a femtosecond laser

Fused silica waveguides were written and reshaped with an 800-mn amplified femtosecond laser in a lateral writing configuration. The asymmetry of the waveguide shape and the index profile was partially corrected through multiple scans shifted by 1 or 2 /spl mu/m. Following the careful adjustment of both laser pulse energy and writing speed, the resulting waveguides exhibited substantial reduction in propagation and coupling losses by more than 50% in the C-band.

Signal attenuation and box-counting fractal analysis of optical coherence tomography images of arterial tissue

The sensitivity of optical coherence tomography images to sample morphology is tested by two methods. The first method estimates the attenuation of the OCT signal from various regions of the probed tissue. The second method uses a box-counting algorithm to calculate the fractal dimensions in the regions of interest identified in the images. Although both the attenuation coefficient as well as the fractal dimension correlate very well with the anatomical features of the probed samples; the attenuation method provides a better sensitivity. Two types of samples are used in this study: segments of arteries collected from atherosclerosis–prone Watanabe rabbits (WHHL-MI) and healthy segments of porcine coronary arteries.

Quadrature Mach?Zehnder interferometer with application in optical coherence tomography

A novel quadrature interferometer based on Mach–Zehnder configuration and a 3 × 3 fibre coupler is presented. Measurements showing instantaneous extraction of both real and complex parts of the interferometric signal are described. The features of this quadrature interferometric platform are discussed with a focus on signal-to-noise ratio and optical power handling.

Optical coherence tomography: fundamental principles, instrumental designs and biomedical applications

The advances made in the last two decades in interference technologies, optical instrumentation, catheter technology, optical detectors, speed of data acquisition and processing as well as light sources have facilitated the transformation of optical coherence tomography from an optical method used mainly in research laboratories into a valuable tool applied in various areas of medicine and health sciences. This review paper highlights the place occupied by optical coherence tomography in relation to other imaging methods that are used in medical and life science areas such as ophthalmology, cardiology, dentistry and gastrointestinal endoscopy. Together with the basic principles that lay behind the imaging method itself, this review provides a summary of the functional differences between time-domain, spectral-domain and full-field optical coherence tomography, a presentation of specific methods for processing the data acquired by these systems, an introduction to the noise sources that plague the detected signal and the progress made in optical coherence tomography catheter technology over the last decade.

Stationary-fiber rotary probe with unobstructed 360° view for optical coherence tomography

A side-scanning fiber probe is a critical component for optical coherence tomography in medical imaging and diagnosis. We propose and fabricate an on-axis rotating probe that performs in situ, circumferential scanning that is shadow-free (not susceptible to shadow effects caused by the motors wires). A miniature motor that incorporates a bored-out shaft for the optical fiber is located at the distal end of the probe, which results in a more stable and uniform circumferential scan, free from wire-shadow interference effects. More importantly, this design, novel to our knowledge, compared to other probes avoids the insertion losses introduced by optical coupling components and the multitude of optical interfaces, which is very important for sensing weak signals backscattered from structures deep in the tissue.

Added soft tissue contrast using signal attenuation and the fractal dimension for optical coherence tomography images of porcine arterial tissue

Optical coherence tomography (OCT) images of left-descending coronary tissues harvested from three porcine specimens were acquired with a home-build swept-source OCT setup. Despite the fact that OCT is capable of acquiring high resolution circumferential images of vessels, many distinct histological features of a vessel have comparable optical properties leading to poor contrast in OCT images. Two classification methods were tested in this report for the purpose of enhancing contrast between soft-tissue components of porcine coronary vessels. One method involved analyzing the attenuation of the OCT signal as a function of light penetration into the tissue. We demonstrated that by analyzing the signal attenuation in this manner we were able to differentiate two media sub-layers with different orientations of the smooth muscle cells. The other classification method used in our study was fractal analysis. Fractal analysis was implemented in a box-counting (fractal dimension) image-processing code and was used as a tool to differentiate and quantify variations in tissue texture at various locations in the OCT images. The calculated average fractal dimensions had different values in distinct regions of interest (ROI) within the imaged coronary samples. When compared to the results obtained by using the attenuation of the OCT signal, the method of fractal analysis demonstrated better classification potential for distinguishing amongst the tissue ROI.