Cristina Caenazzo
University of Padua
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Featured researches published by Cristina Caenazzo.
Diagnostic Molecular Pathology | 1993
Maurizio Onisto; Spiridione Garbisa; Cristina Caenazzo; M. P Freda; C. Di Francesco; D. Nitti; Lance A. Liotta; William G. Stetler-Stevenson
The matrix metalloproteinase enzymes have been implicated in tumor invasion and metastasis by a series of correlative immunohistochemical studies. In addition, direct evidence for the role of these enzymes in this pathologic process comes from studies using specific metalloproteinase inhibitors to block tumor invasion and metastasis formation, both in vitro and in vivo. Synthetic oligonucleotide primers for four metalloproteinases (MMP-1. MMP-2, MMP-9. MMP-10) and their tissue inhibitors (TIMP-1. TIMP-2) were selected, synthesized, and optimized in the reverse transcriptase-polymerase chain reaction (RT-PCR) to study the qualitative profile of these enzymes and inhibitors in cultured human tumor cells and tumor tissues. These primers are specific and generate unique amplification products for each appropriate enzyme and inhibitor. Slight enhancement in the amplification of cDN A products was achieved by adding di-methylsulfoxide to the reaction mixture, but commercial enhancement reagents were ineffective. Using this RT-PCR method, cDNA amplification was successful with RNA from as few as 20 cultured tumor cells. The RT-PCR analysis was done on three invasive human colon adenocarcinomas and their paired adjacent normal mucosa. The results show MMP-1 and MMP-2 products in all three tumors, and MMP-2 detected in one of the three normal mucosa samples; TIMP-2 expression was present in two of three patients and awaits quantitative assessment of RT-PCR products.
Diagnostic Molecular Pathology | 1994
Walter Franco Grigioni; Antonia D'Errico; Michelangelo Fiorentino; Baccarini P; Maurizio Onisto; Cristina Caenazzo; William G. Stetler-Stevenson; Spiridione Garbisa; Antonio M. Mancini
Simultaneous presence of gelatinase A (MMP-2) and MMP-2 messenger RNA (mRNA) in 30 malignant tumors with various degrees of differentiation and biological behavior was evaluated by immunohistochemistry and in situ hybridization. The series consisted of 10 gastric carcinomas. 10 colorectal carcinomas, five squamous skin carcinomas, and five basal cell skin tumors. MMP-2 was detected in all cases. MMP-2 mRNA was expressed in the stromal cells in all cases and was more marked in the less-differentiated gastric and colonic carcinomas; it was also detected in the neoplastic cells of poorly differentiated tumors, particularly in those of the signet-ring cell type, both in the colon and stomach. The study confirmed that stromal cells have a specific role in tumor invasion and suggests a direct relationship between neoplastic epithelium and stromal cells in the most aggressive varieties.
International Journal of Gynecology & Obstetrics | 1989
Maurizio Onisto; S. Fasolato; R. Veggian; Cristina Caenazzo; Spiridione Garbisa
Improvement of human chorionic villi cells in vitro culture has been obtained by supplementing the medium with 30% human fetal cord serum, instead of fetal calf serum. Expression of human chorionic gonadotropin, estrogen and progesterone receptors, laminin, laminin receptors, and type IV collagen has been studied on first subculture passages (4–7 weeks) by immunoperoxidase method. A minority of the cultured cells were positive for estrogen receptors, the majority were positive for progesterone receptors, while all cells were negative for human chorionic gonadotropin. Cultured cells showed variable positive immunostaining for basement membrane markers like laminin, and type IV collagen, and for laminin receptors. Detection of both progesterone receptors and laminin, or type IV collagen, excluded fibroblast contamination and could then be useful for quick identification of cultured trophoblast cells.
Cancer Research | 1992
Spiridione Garbisa; Giorgio V. Scagliotti; Laura Masiero; Carla Di Francesco; Cristina Caenazzo; Maurizio Onisto; Marco Micela; William G. Stetler-Stevenson; Lance A. Liotta
International Journal of Cancer | 1995
Maurizio Onisto; Maria Pia Riccio; Paolo Scannapieco; Cristina Caenazzo; Luciano Griggio; Michele Spina; William G. Stetler-Stevenson; Spiridione Garbisa
The Journal of Infectious Diseases | 1992
Livio Trentin; Spiridione Garbisa; Renato Zambello; Carlo Agostini; Cristina Caenazzo; Carla Di Francesco; Angiolo Cipriani; Ermenegildo Francavilla; Gianpietro Semenzato
Clinical Cancer Research | 1998
Cristina Caenazzo; Maurizio Onisto; Luigi Sartor; Romano Scalerta; Anna Giraldo; Donato Nitti; Spiridione Garbisa
Protein Engineering | 1997
Alessandro Negro; Maurizio Onisto; Luigi Grassato; Cristina Caenazzo; Spiridione Garbisa
Nephrology Dialysis Transplantation | 1997
Cristina Caenazzo; Spiridione Garbisa; Maurizio Onisto; M Zampieri; Bruno Baggio; G. Gambaro
Nephrology Dialysis Transplantation | 2001
Paolo Menè; Cristina Caenazzo; Francesco Pugliese; Giulio A. Cinotti; Angela D'Angelo; Spiridione Garbisa; Giovanni Gambaro