Cuiping Han

Highly fluorescent carbon dots as selective and sensitive "on-off-on" probes for iron(III) ion and apoferritin detection and imaging in living cells.

Highly blue luminescent nitrogen-doped carbon dots (N-CDs) with a fluorescence quantum yield of 42.3% were prepared by an efficient one-step pyrolytic route from ethylenediaminetetraacetic acid and urea. The as-synthesized N-CDs were demonstrated as an effective fluorescent probe for label-free, selective and sensitive recognition of Fe(3+) with a linear range of 0.5μM to 2mM and a detection limit of 13.6nM due to Fe(3+)-quenched fluorescence (turn-off). The quenched fluorescence could be turned on after the addition of apoferritin owing to the removal of ferric species from the surface of N-CDs by apoferritin, making complex N-CDs/Fe(3+) a selective apoferritin probe with a linear range of 0.1-25μM and a detection limit as low as 2.6nM. In addition, the application of this novel N-CDs-based probe for imaging Fe(3+) ions and apoferritin in living cells suggest that this sensing system has great potential applications in biosensing, bioimaging, and many other fields.

Design and synthesis of a highly sensitive “Turn-On” fluorescent organic nanoprobe for iron(III) detection and imaging

Novel naphthalimide fluorescent organic nanoparticles with aggregation-induced emission enhancement properties were designed and synthesized, which were used as a highly selective and sensitive “turn-on” fluorescent probe for Fe3+ detection in water. Fluorescence imaging experiments of Fe3+ ions in living cells demonstrated the value of this probe for practical applications in biological systems.

Fabrication of mAb G250-SPIO Molecular Magnetic Resonance Imaging Nanoprobe for the Specific Detection of Renal Cell Carcinoma In Vitro

Molecular magnetic resonance imaging (mMRI) has been paid more and more attention for early diagnosis of cancer. A sensitive and specific mMRI probe plays the most important role in this technique. In this study, superparamagnetic iron oxide (SPIO) nanoparticles and mAb G250 were conjugated as mMRI probe for the detection of clear cell renal cell carcinoma (ccRCC) using 3.0-Tesla MRI in vitro. mAb G250 could specifically recognize carbonic anhydrase IX (CAIX) antigen overexpressed in ccRCC and the SPIO nanoparticles as MRI contrast agent presented excellent MRI response and good biocompatibility. The successful assembly of this nanoprobe was confirmed by UV-vis spectrum, FT-IR spectroscopy and DLS analysis. In vitro MRI study on ccRCC cells and control cells indicated that our fabricated mAb G250-SPIO nanoprobe could be used in the specific labeling of clear cell renal carcinoma cells successfully.

Synthesis of a multifunctional manganese(II)–carbon dots hybrid and its application as an efficient magnetic-fluorescent imaging probe for ovarian cancer cell imaging

A novel magnetic-fluorescent manganese(ii)-carbon dots hybrid nanoprobe conjugated with anti-HE4 antibody was successfully prepared for ovarian cancer cell targeting and bifunctional optical and MR imaging. In vitro and in vivo studies demonstrated the nanoprobe had high bio-compatibility, excellent tumor-targeting ability, superior MR and fluorescence imaging effects.

Comparison of 3.0T MRI with 3D LAVA sequence and digital subtraction angiography for the assessment of accessory hepatic veins in Budd‐Chiari syndrome

To compare 3D liver acceleration volume acquisition (LAVA) and digital subtraction angiography (DSA) for evaluating the presence of accessory hepatic veins (AHV) in Budd‐Chiari syndrome (BCS).