D. De Orsi

Simultaneous determination of triprolidine, pseudoephedrine, paracetamol and dextromethorphan by HPLC

SummaryA simple, rapid and specific HPLC method has been developed for the determination of triprolidine, pseudoephedrine, paracetamol and dextromethorphan, in combination, in different pharmaceutical dosage forms, using a reversed-phase C18 column, gradient elution, and UV detection at 254 and 280 nm. No preliminary extraction procedure is required for liquid formulations and a very simple extraction procedure is required for tablets and creams. The recovery of the drugs ranged from 96.0 to 98.7%. The assay results obtained for eight commercially available formulations were in agreement with the amounts declared. The linearity and precision of the method have been assessed.

Simultaneous Determination of Antioxidants and Preservatives in Cosmetics and Pharmaceutical Preparations by Reversed-Phase HPLC

A high performance liquid chromatographic method for the simultaneous determination of multiple additives in o/w cosmetic and pharmaceutical formulations was developed by using a RP-8 Select B column, a linear gradient elution and UV detection. A very simple extraction procedure was required. The separation obtained for nine antioxidants and seven preservatives was very good under the chromatographic conditions used. Their analysis was carried out in commercial samples and satisfactory results were obtained both for the recovery and the coefficient of variation.

HPLC determination of rhodamine B (C.I. 45170) in cosmetic products

SummaryA method is proposed for the extraction, separation, identification and quantitative measurement of rhodamine B in cosmetics samples The colour is extracted with a solution of orthophosphoric acid in either water or DMF (depending on the type of cosmetic) adsorbed on a polyamide column, and then eluted with water and methanol. Further clean-up of the extract is performed by means of a Bond Elut C-18 cartridge, from which rhodamine B is eluted with methanol-water (8∶2). The final eluate is examined by HPLC. Chromatography is performed on a C-18 column with a gradient elution from 50∶50 to 70∶30 of acetonitrile-water, containing 0.1 M sodium perchlorate, in 15 min. A diodearray detector enables peak purity analysis. The method gives recovery values of approximately 90%, or better, and the reproducibility is within 4%.

HPLC DETERMINATION OF CLOBETASOL PROPIONATE IN COSMETIC PRODUCTS

A simple and rapid HPLC method for the determination of clobetasol 17-propionate in cosmetic formulations, with antidranduff and antiseborrheic activity, has been developed. The chromatographic separation was carried out on a 5 μm Purospher-Lichrocart column using water-acetonitrile (40:60, v/v) as the mobile phase and UV detection at 237 nm. The extraction procedure has been validated analysing samples spiked with known amounts of the active principle. The recoveries were greater than 94% and the reproducibility was within 3.0%.

Simultaneous determination of ephedrine and 2-imidazolines in pharmaceutical formulations by reversed-phase HPLC

Abstract A simple, rapid, and accurate method for the determination of naphazoline, oxymetazoline, xylometazoline, and ephedrine in rinological solutions using reversed-phase HPLC was developed. It involves the use of alumina coated with polybutadiene as the stationary phase, acetonitrile-aqueous 10−3 M NaOH (10:90) as the initial mobile phase with a linear gradient from 10 to 80% acetonitrile in 25 min, and detection at 224 nm. The only sample preparation is its dilution with water. Linearity and precision of the method have been assessed. The assay results obtained for various formulations were in agreement with the declared amounts.

HPLC Determination of Beclomethasone Dipropionate and Its Degradation Products in Bulk Drug and Pharmaceutical Formulations

An HPLC method for the simultaneous determination of beclomethasone dipropionate and its principal degradation products has been developed. The only sample treatment necessary for the analysis is its dilution with methanol. The practical detection limit is 0.02 % of each impurities in terms of the parent drug, whereas the absolute limit of detection is 2.5 ng of each injected compound. The method has been applied to the analysis of both pharmaceutical formulations and samples of bulk drug.

High-performance liquid chromatographic determination of urocanic acid isomers in cosmetic products

SummaryA method has been developed for the determination of trans and cis urocanic acid in oil-in-water cosmetic emulsions. It involves an extraction of the sample in 1:3 methanol-aqueous NaOH (10−3 M), by ultrasonication, which leads to quantitative recoveries, and a reversed-phase HPLC isocratic elution for the analysis of the extract. Chromatography is performed on a C18 column using 0.1 M sodium perchlorate (pH 3.0)-acetonitrile 98:2 (v/v), as the mobile phase, and UV detection at 263 nm. The separation of the isomers is good. Linearity and precision of the method have been assessed.

HPLC Determination of Ciclopirox, Octopirox, and Pyrithiones in Pharmaceuticals and Antidandruff Preparations

A simple and rapid HPLC method for the determination of ciclopirox, octopirox, and pyrithiones in pharmaceutical or antidandruff formulations has been developed. HPLC was carried out on a Purospher® RP-18 endcapped column using acetonitrile-water containing 0.02 M ortophosphoric acid and 0.5 mM EDTA disodium salt, (68:32, v/v) as the mobile phase and UV detection at 305 or 340 nm. The extraction procedure has been validated analysing samples spiked with known amounts of the active principles. The recoveries were greater than 94% and the reproducibility was within 3%.

HPLC Determination of Oxiracetam, Its Impurities, and Piracetam in Pharmaceutical Formulations

Abstract The aim of this work was to develop an high performance liquid chromatographic method for the determination of oxiracetam and its impurities in samples of bulk drugs and pharmaceutical preparations. The only sample preparation necessary for the analysis was its dilution with the mobile phase. Due to the chemical and pharmacological similarity, also the active ingredient piracetam was considered. The method was applied to commercial samples.

HPLC analysis of boldine in pharmaceuticals

SummaryA method is proposed for extraction, separation, identification and quantitative measurement of boldine (I) in pharmaceuticals. The extraction of (I) from the matrix (syrups, tablets, extracts) was realized with aqueous ammonium chloride (0.02 M, pH=3.0 with perchloric acid) and the clean-up of the extract was performed by solid-phase extraction by means of cartridges containing a strong cation-exchange sorbent, from which (I) was eluted with aqueous ammonium chloride (1 M, pH=10.0 with ammonia). The final eluate was examined by HPLC. Chromatography was performed on a C-8 column with a gradient elution from 15:85 to 30:70 of acetonitrile-water containing 0.1 M sodium perchlorate (pH=3.0), in 20 min. A diode array detector was used at 302 nm for detection. The method gives recovery values greater than 98% and the reproducibility is within 6.0%.