D. L. Hopkins

Relationship between consumer ranking of lamb colour and objective measures of colour.

Given the lack of data that relates consumer acceptance of lamb colour to instrument measures a study was undertaken to establish the acceptability thresholds for fresh and displayed meat. Consumers (n=541) were asked to score 20 samples of lamb loin (m.longissimus thoracis et lumborum; LL) on an ordinal scale of 1 (very acceptable) to 5 (very unacceptable). A sample was considered acceptable by a consumer if it scored three or less. Ten samples were used for testing consumer response to fresh colour and 10 to test consumer response to colour during display of up to 4days. The colour of fresh meat was measured using a Minolta chromameter with a closed cone and a Hunter Lab Miniscan was used for measuring meat on display. For fresh meat when the a( *) (redness) and L( *) (lightness) values are equal to or exceed 9.5 and 34, respectively, on average consumers will consider the meat colour acceptable. However a( *) and L( *) values must be much higher (14.5 and 44, respectively) to have 95% confidence that a randomly selected consumer will consider a sample acceptable. For aged meat, when the wavelength ratio (630/580nm) and the a( *) values are equal to or greater than 3.3 and 14.8, respectively, on average consumers will consider the meat acceptable. These thresholds need to be increased to 6.8 for ratio (630/580nm) and 21.7 for a( *) to be 95% confident that a randomly selected consumer will consider a sample acceptable.

Relationship between animal age, intramuscular fat, cooking loss, pH, shear force and eating quality of aged meat from sheep

The relationships between sensory traits (tenderness, juiciness, flavour and overall liking) and objective measures, such as shear force, intramuscular fat, cooking loss, pH and animal age, were derived for M. longissimus thoracis et lumborum (LL) from 471 lamb and sheep carcasses. Tenderness could be predicted with the most accuracy (R2 = 0.24) and flavour with the highest precision (r.s.d. = 7.5 units) when using the objective measures, which may be in part due to the small variation in the range of shear force values of the samples (all carcasses electrically stimulated and meat aged for 5 days) and the use of consumer panels for the assessment of sensory traits. The ultimate pH of the LL, the rate of decline in pH in the LL or the predicted temperature at pH 6.0 were not significant predictors of the sensory traits when tested on a subsample of the carcasses. The model coefficients indicated that all sensory traits (tenderness, flavour, juiciness and overall liking) declined as shear force and age increased, and as intramuscular fat percentage decreased. This translated into a decline of 16 points on average for tenderness and 13 points for overall liking when LL samples from 68.5-month-old sheep were compared with those from unweaned lambs, when adjusted to the same level of intramuscular fat and shear force. Predictions of the sensory traits at varying levels of shear force were made and show that at 49 Newtons (N), the overall liking score would be 51 and the tenderness score 48. Derived relationships between objective meat quality measures and sensory traits suggest that to achieve a failure rate of no more than 10% for loin meat when eaten, it must have a shear force of about 27 N or less.

The impact of processing on sensory and objective measurements of sheep meat eating quality

The impact of processing factors on sheep meat eating quality was investigated in an experiment in which stimulation (stimulation and no stimulation), chilling rate (fast and slow chilling) and carcass suspension (tenderstretch and normally hung by Achilles tendon) treatments were overlaid on 80 lamb and 40 mutton carcasses processed over 2 days, at 2 different abattoirs. Within each carcass, 3 muscles (M. longissimus thoracics et lumborum, biceps femoris and serratus ventralis) were collected from both sides and aged for 2 of 3 ageing periods (2, 5 or 14 days), before sensory testing for tenderness, juiciness, like flavour and overall liking, using a consumer taste panel. Processing treatments of stimulation and chilling were variable in their effect on the rates of pH and temperature decline and temperature at pH 6 (temp@pH6), between the 4 slaughter groups. Therefore chilling rate and electrical stimulation were considered as tools by which temp@pH6 could be manipulated, rather than as treatment effects per se. Age category (lamb or mutton) had the largest impact on tenderness score, followed by muscle, ageing and carcass suspension. There were significant interactions for tenderness and overall liking scores between muscle × ageing, age category × muscle and carcass suspension × muscle (P 30°C) or low (<10°C) temp@pH6. There was a tendency for sensory scores (particularly for the like flavour score) to decline for the biceps femoris when it was stored for extended periods (14 days).

Genotype and age effects on sheep meat production. 3. Meat quality

A 5 × 4 factorial experiment was designed in which lambs representing five genotypes were slaughtered at four ages (110, 236, 412 and 662 days of age or 4, 8, 14 and 22 months of age). The genotypes represented were Poll Dorsetgrowth × Border Leicester Merino (PDg × BLM), Poll Dorsetgrowth × Merino (PDg × M), Poll Dorsetmuscling × Merino (PDm × M), Merino × Merino (M × M) and Border Leicester × Merino (BL × M). In total, 595 animals were slaughtered and carcass traits, composition and meat quality measured. As the animals grew older, intramuscular fat percentage (IMF) increased in the longissimus (LL) muscle and as the proportion of Border Leicester parentage increased so did the levels of IMF. There was a significant sire breed effect on LL pH at 24 hours after slaughter (pH24), with Merino-sired animals having a higher pH24 than both Poll Dorset and BL-sired animals. A significant dam breed effect was found for pH24 of the semitendinosus (ST) muscle, with lambs born to Merino ewes having a higher pH than those born to BLM ewes. A significant effect of sire estimated breeding value (EBV) for yearling muscling (YEMD) on LL pH was detected indicating an increase in pH as the sire EBV for muscling increased. There was a significant Merino effect for ultimate pH of the ST, with Merino-sired animals having a higher ultimate pH than both Poll Dorset and Border Leicester-sired animals. Sucker lambs had a significantly lower ST pH than older animals. As animal age at slaughter increased, the LL became darker based on L* values. There was a significant increase in a* values as animals became older, with 14 and 22-month-old animals having redder muscle. As LL pH and YEMD EBV increased, a* values decreased. There was a significant interaction between animal age and post mortem aging time such that sucker lambs (4 months old) produced LL with the lowest shear force after 5 days of aging and exhibited the greatest decline in shear force with aging. There was a significant increase in semimembranosus muscle shear force for PDm-sired animals (mean ± s.e., 66.5 ± 1.99 Newtons) compared with other genotypes at 61.4 ± 1.28 Newtons, but this could not be attributed to YEMD.

Development of a sensory protocol for testing palatability of sheep meats

A total of 108 grill and 108 roast samples were prepared from lamb (n = 10) and mutton (n = 8) carcasses for sensory testing using a consumer taste panel. Grill and roast samples were prepared from the left and right sides of the carcass, respectively, using longissimus, biceps femoris, gluteus medius, serratus ventralis and semimembranosus. Due to size constraints, muscle from both sides was used to form grill samples for the vastus lateralis, and roast samples from the triceps brachii. Grill and roast samples were sensory tested using 360 untrained consumers. Each consumer was given a total of 6 experimental samples and each sample was tested by 10 different consumers. Sensory scores for tenderness, juiciness, like flavour and overall liking from both the grilled and roasted samples were highly correlated (P 0.05). In contrast, juiciness and like flavour scores were poorly correlated between grilled muscles. For the roasted samples, sensory scores were generally uncorrelated between muscles. The statistical significance of the age category and muscle effects was greater in grill samples, but stimulation effects were of similar significance using either cooking method. It was proposed that roasting reduced treatment effects that affected sensory via differences in connective tissue toughness, due to gelatinisation of connective tissue during cooking. For testing production and processing effects on palatability, grilling was more sensitive for detecting treatment effects, than roasting.

Relationship between sire estimated breeding values and the meat and eating quality of meat from their progeny grown on two planes of nutrition

The meat of 140 female lambs was examined. These lambs were sired by 9 Poll Dorset sires (3 selected for growth, 3 for muscling and 3 control) and fed either a low or high plane of nutrition from birth to slaughter. All carcasses were electrically stimulated and portions of M. longissimus thoracis et lumborum (loin) aged for 5 days before freezing. Subsequently, 10 consumers tested each portion for eating quality traits. A portion of the same muscle from the opposing side of the carcass was divided into 3 sections and aged for either 1, 3 or 5 days and then used to measure shear force (objective tenderness). Portions of the M. semimembranosus (topside) were also aged for 3 or 5 days and subjected to shear testing. Low plane animals produced tougher loins (based on shear force). As ultimate pH increased, shear force of the loin increased for low plane animals, while for high plane animals there was no such effect. This latter group had significantly lower ultimate pH values in all 3 muscles tested. Plane of nutrition had the greatest effect on topside shear force with low plane lambs producing tougher topsides. As ultimate pH increased, the topsides became tougher and as expected ageing improved the tenderness. There was no effect of sire EBVs (estimated breeding values) on shear force or objective meat quality traits apart from loin lightness which increased as the EBV for post-weaning weight (PWWT) increased and intramuscular fat in the loin which decreased as sire EBV for post-weaning muscle depth (PEMD) increased. Plane of nutrition did not have a significant effect on eating quality attributes as assessed by consumers. There was, however, an effect of sire EBV for muscling (PEMD) on tenderness, juiciness, flavour and overall liking, such that increased EBV led to decreased scores for the sensory traits. The findings suggest selection of sires for increasing PEMD will have a detrimental affect on the eating quality of their progeny, but this will not be reflected in shear force values or other objective meat quality traits apart from intramuscular fat. The effects of a low plane of nutrition on eating quality were minimal and largely controlled by the use of best practice processing, but were evident for a number of meat quality traits.

Myofibre characteristics of ovine longissimus and semitendinosus muscles are influenced by sire breed, gender, rearing type, age and carcass weight

The objective of this study was to determine the extent to which sire breed (Merino, primarily a wool-producing breed, and Poll Dorset, primarily a meat-producing breed), gender (castrate and ewe), rearing type (single- or multiple-reared), age (4, 8, 14 and 22 months) and carcass weight influence ovine (n = 204) myofibre characteristics. In Merino-sired sheep, the relative area (% of total myofibre area) of type 2X myofibres was lower and of type 2A myofibres higher in the longissimus lumborum (longissimus) muscle than in Poll Dorset-sired sheep. Female sheep had a higher relative area of type 2X and a lower relative area of type 2A myofibres than castrate sheep in the longissimus muscle. The percentage of type 2A myofibres increased and of type 2X myofibres decreased in the longissimus between 4 and 22 months of age. Type 1 myofibres in the semitendinosus muscle were more prevalent and type 2X myofibres less prevalent in Merino than in Poll Dorset-sired sheep. The percentage of type 1 myofibres was lower in female than in castrate sheep. Age-related effects on semitendinosus myofibre characteristics varied with gender, sire breed and rearing type. Influences of rearing type on myofibre characteristics were evident in the semitendinosus muscle at younger ages, but did not persist to 22 months of age. The greatest increases in myofibre cross-sectional areas occurred between 8 and 14 months of age in the longissimus muscle, and between 14 and 22 months of age in the semitendinosus muscle. Carcass weight significantly influenced cross-sectional area of type 2A, 2AX and 2X myofibres in longissimus and semitendinosus muscles. It is concluded that influences of sire breed, gender, rearing type, age and carcass weight on ovine myofibre percentages varies between longissimus and semitendinosus muscles. Furthermore, the pattern of postnatal myofibre growth differs between these muscles.

Preliminary estimates of genetic parameters for carcass and meat quality traits in Australian sheep.

Using performance from progeny born in 2007 and 2008 generated by theInformation Nucleus program of the Cooperative Research Centre for Sheep Industry Innovation, preliminary estimates of heritability were obtained for a rangeofnovelcarcassandmeatattributesoflambrelevanttoconsumers,includingcarcasscharacteristics,meatqualityand nutritionalvalueoflamb.Phenotypicandgeneticcorrelationsofliveanimaltraitswithcarcasscompositionandmeatquality traits were also estimated. The data were from progeny located at eight sites, sired by 183 rams from Merino, maternal and terminalmeatbreedsandwererepresentative oftheMerino,BorderLeicester ·Merino,Terminal ·MerinoandTerminal · Border Leicester-Merino production types of the Australian sheep industry. Data were available from 7176 lambs for weaning weight, 6771 lambs for ultrasound scanning and 4110 lambs for slaughter traits. For the novel meat quality traits, generally moderate to high heritability estimates were obtained for meat quality measures of shear force (0.27 aged 1 day, 0.38 aged 5 days), intramuscular fat (0.39), retail meat colour (range of 0.09 to 0.44) and myoglobin content (0.22). The nutritional value traits of omega-3 fatty acids and iron and zinc contents tended to have low to moderate heritabilities (0.11-0.37), although these were based on fewer records. Fresh meat colour traits were of low to moderate heritability (0.06-0.21)whereasmeasuresofmeatpHwereoflowheritability(~0.10).Forthecarcasstraits,estimatesofheritabilitywere moderate to high for the various measures of carcass fat (0.18-0.50), muscle weight (0.22-0.35), meat yield (0.24-0.35), carcassmuscledimensions(0.25-0.34)andboneweight(0.27).Resultsindicatethatformostlambcarcassandmeatquality traits there is sufficient genetic variation for selection to alter successfully these characteristics. Additionally, most genetic correlations of live animal assessments of bodyweight, muscle and subcutaneousfat with the carcass and meat quality traits werefavourable.Appropriatedefinitionofbreedingobjectivesanddesignofselectionindexesshouldbeabletoaccountfor the small unfavourable relationships that exist and achieve the desired outcomes from breeding programs.

Influence of finishing systems and sampling site on fatty acid composition and retail shelf-life of lamb

Thirty 7-month-old crossbred lambs (Poll Dorset × Border Leicester × Merino) finished over 5 weeks on either low quality pasture or grain-with-hay on a farm in southern Victoria were assessed for carcass parameters, muscle fat composition and retail colour stability. Lambs on the grain diet had a mix of barley grain (80%) and lentils (20%) at 800 g/head.day (air-dry basis ~ad libitum) with cape weed (Arctotheca calendula) hay available at all times. Lambs under grazing had predominantly rye grass (Lolium perenne) and barley grass (Hordeum leporinum) available ad libitum. Carcass weight tended to be higher (P = 0.14) in grain-fed lambs than in grass-fed lambs, but fatness indicated by GR (total muscle + fat tissue thickness at 11 cm from midline) did not differ between feeding systems. Fatty acid composition was determined in the loin from the forequarter (M. longissimus thoracis) and lumbar (M. longissimus lumborum) regions and from the leg region (M. semimembranosus). This showed that grain-finished lamb had higher muscle fat (P 0.05) between feeding groups. Saturated fatty acids were greater (P < 0.01) in both loin sampling sites than the leg. The levels of EPA, docosahexaenoic acid and docosapentaenoic acid were lower (P < 0.01) in the forequarter (9%) or lumbar (11%) sites than the leg sampling site. The distribution pattern of fatty acids across the three sampling sites did not differ between feed types. Retail colour stability determined over 4 days of display (only performed in muscle from the lumbar site), evaluated by the redness (HunterLab a*-value) and metmyoglobin formation (reflectance ratio at 630 : 580-nm wavelengths) was superior for grass-fed lamb compared with short-term grain-finished lamb. The results demonstrate that the health claimable omega-3 fat mainly EPA or total omega-3 fat content in lamb was not altered by short-term grain finishing compared with lamb finished under pasture grazing conditions.

Sire and growth path effects on sheep meat production. 2. Meat and eating quality

The meat and eating quality characteristics of 627 crossbred lamb progeny from 20 Poll Dorset sires were studied. The sires were selected on the basis of Australian sheep breeding values (ASBVs) for postweaning growth (PWWT), depth of loin muscle (PEMD) and subcutaneous fat (PFAT). Lambs were either weaned at 20- or 30-kg liveweight and then within each of these groups held at their weaning weight for 55 days or fully fed, giving four treatment groups. Restricted lambs were realimentated subsequent to the period of feed restriction and the lambs within each treatment group were slaughtered when their mean liveweight reached 45 kg. Shear force of the M. longissimus thoracis et lumborum (LL) decreased with temperature at pH 6.0, increased with LL ultimate pH and decreased as sarcomere length increased. Within LL aged for 5 days there was no difference between (P > 0.05) between treatments for shear force. There was a treatment effect on M. semimembranosus (SM) shear force, such that for a sire having an average ASBV PWWT of 9.34, topsides from lambs early weaned and restricted had significantly (P < 0.05) lower values. Further within these same lambs, i.e. early weaned and restricted, as the sire ASBV PWWT increased there was a significant (P < 0.001) reduction in shear force. There was a significant (P < 0.05) increase in sensory tenderness as the sire ASBV PFAT increased and across the range of ASBV PFAT this represented a change in sensory tenderness of four points. Overall liking increased with an increase in the temperature at pH 6.0 (P < 0.05) with a coefficient of 0.43 ± 0.19. There was a significant (P < 0.05) interaction between feeding level and the sire ASBV PFAT, such that for restricted fed lambs overall liking increased as the ASBV PFAT increased (coefficient 2.80 ± 0.89) after adjusting for temperature at pH 6.0, but this is not the case for fully feed lambs (coefficient -0.01 ± 0.89). Subtle interactions between sire ASBVs and growth path suggest that the impact of a period of restriction and refeeding on meat and eating quality will be meditated by the sire genetics.