D. Talwar

OP020 QUANTITATIVE DATA ON THE MAGNITUDE OF THE SYSTEMIC INFLAMMATORY RESPONSE AND ITS EFFECT ON VITAMINS B1, B2 AND B6 BASED ON RED CELL MEASUREMENTS

Rationale: There is good evidence that plasma concentrations of vitamins B2 and B6 fall as part of the systemic inflammatory response (SIR, 1, 2). In order to address this issue we have introduced the use red cell measurements B1 (Thiamine diphosphate), B2 (Flavin adenine dinucleotide) and B6 (pyridoxal phosphate) and this study examines the effect of the magnitude of the SIR on these red cell measurements. Methods: Between 2008 and 2012 results in which there were measures of B1 (n = 3252), B2 (n = 1947), B6 (n = 1958) and CRP were examined. B vitamins were categorised into 6 CRP groups ( 5, >5 10, >10 20, >20 40, >40 80 and >80mg/l). Results: Over the increasing CRP groups (from 5 to >80mg/l) B1 concentrations increased from median 551 to median 713 ng/g Hb (29%, p < 0.001); B2 concentrations increased from median 1.70 to median 1.90 pmol/gHb (12%, p < 0.001); B6 concentrations increased from median 585 to median 717 pmol/gHb (23%, p = 0.014). This was in contrast to a 37% fall reported in the plasma B2 [1] and a 69% fall reported in B6 [2] concentrations over a similar range of CRP concentration. Conclusion: In contrast to plasma measurements the use of red cell measurements prevents misclassification of vitamin B2 and B6 status. The relatively small increases in these red cell measurements with increasing CRP concentrations probably reflects the redistribution of these vitamins from the plasma compartment to the intracellular compartment as part of the SIR.