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Dive into the research topics where D. V. Kopytova is active.

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Featured researches published by D. V. Kopytova.


The EMBO Journal | 2007

SAGA and a novel Drosophila export complex anchor efficient transcription and mRNA export to NPC

M. M. Kurshakova; A. N. Krasnov; D. V. Kopytova; Yulii V. Shidlovskii; Julia V. Nikolenko; E. N. Nabirochkina; Danièle Spehner; Patrick Schultz; Laszlo Tora; S. G. Georgieva

SAGA/TFTC‐type multiprotein complexes play important roles in the regulation of transcription. We have investigated the importance of the nuclear positioning of a gene, its transcription and the consequent export of the nascent mRNA. We show that E(y)2 is a subunit of the SAGA/TFTC‐type histone acetyl transferase complex in Drosophila and that E(y)2 concentrates at the nuclear periphery. We demonstrate an interaction between E(y)2 and the nuclear pore complex (NPC) and show that SAGA/TFTC also contacts the NPC at the nuclear periphery. E(y)2 forms also a complex with X‐linked male sterile 2 (Xmas‐2) to regulate mRNA transport both in normal conditions and after heat shock. Importantly, E(y)2 and Xmas‐2 knockdown decreases the contact between the heat‐shock protein 70 (hsp70) gene loci and the nuclear envelope before and after activation and interferes with transcription. Thus, E(y)2 and Xmas‐2 together with SAGA/TFTC function in the anchoring of a subset of transcription sites to the NPCs to achieve efficient transcription and mRNA export.


Molecular and Cellular Biology | 2006

Two Isoforms of Drosophila TRF2 Are Involved in Embryonic Development, Premeiotic Chromatin Condensation, and Proper Differentiation of Germ Cells of Both Sexes

D. V. Kopytova; Aleksey N. Krasnov; Marina R. Kopantceva; E. N. Nabirochkina; Julia V. Nikolenko; Oksana Maksimenko; M. M. Kurshakova; Lubov Lebedeva; Maksim M. Yerokhin; O. B. Simonova; L. I. Korochkin; Laszlo Tora; Pavel Georgiev; S. G. Georgieva

ABSTRACT The Drosophila TATA box-binding protein (TBP)-related factor 2 (TRF2 or TLF) was shown to control a subset of genes different from that controlled by TBP. Here, we have investigated the structure and functions of the trf2 gene. We demonstrate that it encodes two protein isoforms: the previously described 75-kDa TRF2 and a newly identified 175-kDa version in which the same sequence is preceded by a long N-terminal domain with coiled-coil motifs. Chromatography of Drosophila embryo extracts revealed that the long TRF2 is part of a multiprotein complex also containing ISWI. Both TRF2 forms are detected at the same sites on polytene chromosomes and have the same expression patterns, suggesting that they fulfill similar functions. A study of the manifestations of the trf2 mutation suggests an essential role of TRF2 during embryonic Drosophila development. The trf2 gene is strongly expressed in germ line cells of adult flies. High levels of TRF2 are found in nuclei of primary spermatocytes and trophocytes with intense transcription. In ovaries, TRF2 is present both in actively transcribing nurse cells and in the transcriptionally inactive oocyte nuclei. Moreover, TRF2 is essential for premeiotic chromatin condensation and proper differentiation of germ cells of both sexes.


Nucleic Acids Research | 2012

The DUBm subunit Sgf11 is required for mRNA export and interacts with Cbp80 in Drosophila

Dmitriy Ya. Gurskiy; Anastasija V. Orlova; Nadezhda E. Vorobyeva; E. N. Nabirochkina; A. N. Krasnov; Yulii V. Shidlovskii; S. G. Georgieva; D. V. Kopytova

SAGA/TFTC is a histone acetyltransferase complex that has a second enzymatic activity because of the presence of a deubiquitination module (DUBm). Drosophila DUBm consists of Sgf11, ENY2 and Nonstop proteins. We show that Sgf11 has other DUBm-independent functions. It associates with Cbp80 component of the cap-binding complex and is thereby recruited onto growing messenger ribonucleic acid (mRNA); it also interacts with the AMEX mRNA export complex and is essential for hsp70 mRNA export, as well as for general mRNA export from the nucleus. Thus, Sgf11 functions as a component of both SAGA DUBm and the mRNA biogenesis machinery.


Nucleic Acids Research | 2015

Telomeric repeat silencing in germ cells is essential for early development in Drosophila

Valeriya Morgunova; Natalia Akulenko; Elizaveta Radion; Ivan Olovnikov; Yuri Abramov; Ludmila V. Olenina; Sergey Shpiz; D. V. Kopytova; S. G. Georgieva; Alla Kalmykova

The germline-specific role of telomeres consists of chromosome end elongation and proper chromosome segregation during early developmental stages. Despite the crucial role of telomeres in germ cells, little is known about telomere biology in the germline. We analyzed telomere homeostasis in the Drosophila female germline and early embryos. A novel germline-specific function of deadenylase complex Ccr4-Not in the telomeric transcript surveillance mechanism is reported. Depletion of Ccr4-Not complex components causes strong derepression of the telomeric retroelement HeT-A in the germ cells, accompanied by elongation of the HeT-A poly(A) tail. Dysfunction of transcription factors Woc and Trf2, as well as RNA-binding protein Ars2, also results in the accumulation of excessively polyadenylated HeT-A transcripts in ovaries. Germline knockdowns of Ccr4-Not components, Woc, Trf2 and Ars2, lead to abnormal mitosis in early embryos, characterized by chromosome missegregation, centrosome dysfunction and spindle multipolarity. Moreover, the observed phenotype is accompanied by the accumulation of HeT-A transcripts around the centrosomes in early embryos, suggesting the putative relationship between overexpression of telomeric transcripts and mitotic defects. Our data demonstrate that Ccr4-Not, Woc, Trf2 and Ars2, components of different regulatory pathways, are required for telomere protection in the germline in order to guarantee normal development.


Cell Cycle | 2010

ENY2: couple, triple...more?

D. V. Kopytova; A. N. Krasnov; Anastasija V. Orlova; Dmitriy Ya. Gurskiy; E. N. Nabirochkina; S. G. Georgieva; Yulii V. Shidlovskii

ENY2/Sus1, a protein involved in the coupling of transcription with mRNA export, is a component of SAGA/TFTC and TREX-2/AMEX complexes. Recently, we have described the association of ENY2 with the third protein complex, THO. Moreover, our data indicate that ENY2 is also associated with other factors, both in the nucleus and cytoplasm. Thus, being a shared components of several protein complexes, ENY2 appears to function as an adapter molecule involved in integration of cellular processes, in particular, subsequent stages of gene expression.


Nucleic Acids Research | 2016

ORC interacts with THSC/TREX-2 and its subunits promote Nxf1 association with mRNP and mRNA export in Drosophila

D. V. Kopytova; V. V. Popova; M. M. Kurshakova; Yulii V. Shidlovskii; E. N. Nabirochkina; Alexander V. Brechalov; Georgii P. Georgiev; S. G. Georgieva

Abstract The origin recognition complex (ORC) of eukaryotes associates with the replication origins and initiates the pre-replication complex assembly. In the literature, there are several reports of interaction of ORC with different RNAs. Here, we demonstrate for the first time a direct interaction of ORC with the THSC/TREX-2 mRNA nuclear export complex. The THSC/TREX-2 was purified from the Drosophila embryonic extract and found to bind with a fraction of the ORC. This interaction occurred via several subunits and was essential for Drosophila viability. Also, ORC was associated with mRNP, which was facilitated by TREX-2. ORC subunits interacted with the Nxf1 receptor mediating the bulk mRNA export. The knockdown of Orc5 led to a drop in the Nxf1 association with mRNP, while Orc3 knockdown increased the level of mRNP-bound Nxf1. The knockdown of Orc5, Orc3 and several other ORC subunits led to an accumulation of mRNA in the nucleus, suggesting that ORC participates in the regulation of the mRNP export.


Russian Journal of Genetics | 2003

P-Ph-Mediated Repression of the leg–arista–wing complexGene Transcription in Drosophila

E. A. Modestova; D. V. Kopytova; S. G. Georgieva; O. B. Simonova

Preliminary analysis of the leg–arista–wing complex (lawc) gene region in the corresponding mutants revealed Pelement insertion in the transcribed region of this locus. To demonstrate the main role of P element insertion in the complex pleiotropic phenotypic effect of the lawc gene, a system using P-Ph chimeric protein and based on the P-mediated repression of the lawc in vivo transcription was applied. As a result, extreme lawc-mutant phenotypes were obtained and examined. The P-Ph-mediated decrease of the level of the lawc gene transcription was also demonstrated.


Molecular Biology | 2016

Protein complexes coordinating mRNA export from the nucleus into the cytoplasm

M. M. Kurshakova; S. G. Georgieva; D. V. Kopytova

The molecular mechanisms that coordinate transcription, processing, mRNP assembly, and mRNA export from the nucleus through nuclear pores into the cytoplasm have been the focus of intense research in recent years. Data demonstrating a tight association between the processes involved in gene expression are considered. The main protein complexes that play a role in mRNA export are described. The complexes are recruited to mRNA at steps preceding the mRNA export. The functions that the complexes perform at particular steps of gene expression are analyzed, and protein complexes responsible for quality control of mRNP discussed.


Molecular Biology | 2017

RNA immunoprecipitation technique for Drosophila melanogaster S2 cells

Z. M. Kachaev; R. A. Gilmutdinov; D. V. Kopytova; A. A. Zheludkevich; Yu. V. Shidlovskii; A. S. Kurbidaeva

RNA-binding proteins play an important role in RNA metabolism, especially in mRNA biogenesis and subsequent expression patterns regulation. RNA immunoprecipitation (RIP) is a powerful tool for detecting protein–RNA associations. In this paper, we briefly cover the history of this method for analyzing RNA–protein interactions and reviewing a number of modifications of the RIP technique. We also present an adjusted RIP protocol that was modified for Drosophila S2 cell culture. The use of this protocol allows one to perform the efficient precipitation of RNA–protein complexes and harvest RNA in amounts that are sufficient for its downstream analysis.


Molecular Biology | 2015

Methods to study the RNA-protein interactions

V. V. Popova; M. M. Kurshakova; D. V. Kopytova

RNA-binding proteins (RBPs) play an important role in regulating gene expression at the posttranscriptional level, including the steps of pre-mRNA splicing, polyadenylation, mRNA stabilization, mRNA export from the nucleus to the cytoplasm, mRNA localization, and translation. RBPs regulate these processes primarily by binding to specific sequence elements in newly synthesized or mature transcripts. While many RPBs are known to recognize certain nucleotide sequences in RNA, information is insufficient for others. In particular, RBPs often compete for RNA binding or interact with RNA cooperatively. Hence, it is of importance to study the RNA-protein interactions in vivo. Numerous methods have been developed to identify the target nucleotide sequences of RBPs. The methods include the electrophoretic mobility shift assay (EMSA), systematic evolution of ligands by exponential enrichment (SELEX), RNA pull-down assay, RNA footprinting, RNA immunoprecipitation (RIP), UV-induced crosslinking immunoprecipitation (CLIP) and its variants, and measurement of the level for newly synthesized transcripts. Each of the methods has its limitation, and several methods supplementing each other should be employed in order to detect the RNA sequence to which a protein binds.

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S. G. Georgieva

Russian Academy of Sciences

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E. N. Nabirochkina

Russian Academy of Sciences

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A. N. Krasnov

Russian Academy of Sciences

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M. M. Kurshakova

Russian Academy of Sciences

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V. V. Popova

Russian Academy of Sciences

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Yu. V. Shidlovskii

Russian Academy of Sciences

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D. Ya. Gurskiy

Russian Academy of Sciences

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Julia V. Nikolenko

Russian Academy of Sciences

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