Da-Eun Kim
Chungbuk National University
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Publication
Featured researches published by Da-Eun Kim.
Journal of Plant Biology | 2011
Abu Hena Mostafa Kamal; Ill-Dong Jang; Da-Eun Kim; Tatsuro Suzuki; Keun-Yook Chung; Jong-Soon Choi; Moon-Soon Lee; Cheol-Ho Park; Sang-Un Park; Seong Hee Lee; Heon Sang Jeong; Sun-Hee Woo
We used proteomics analysis to generate the profiles of proteins in the endosperm and embryo of common buckwheat grains. These differentially expressed proteins are potentially involved in seed metabolism. Extractions were done by trichloroacetic acid (TCA) precipitation. The resulting proteins were separated using SDS-PAGE coupled to LC-ESI-Q/TOF-MS/MS. This allowed us to detect and identify 67 proteins with isoforms, making this the most inclusive protein profile. The proteins were determined to be functionally involved in the central metabolic pathway of the seed, with metabolic interest being reflected in the occurrence of a tissue-specific enzyme balance. For a case in point, we found a tissue-specific and subcellular compartment-specific isoform of granule-bound starch synthase 1 in the chloroplast/amyloplast. This provided proteomic verification of the presence of a distinct regulatory mechanism for the biosynthesis of glycan and starch, which produce amylase and amylopectin. Furthermore, several previously characterized allergenic proteins such as 11S and 13S globulin seed storage protein were acknowledged in our seed samples, thus representing the potential for proteomics techniques that survey food sources for any incidence of allergens. This protein profile of common buckwheat grain is a new avenue for understanding its seed physiology in dormant stage as well as suggesting commercial applications for the buckwheat industry as buckwheat flour.
Molecular Biology Reports | 2012
Abu Hena Mostafa Kamal; Kun Cho; Da-Eun Kim; Nobuyuki Uozumi; Keun-Yook Chung; Sang-Young Lee; Jong-Soon Choi; Seong-Woo Cho; Chang-Seob Shin; Sun Hee Woo
African Journal of Biotechnology | 2010
Abu Hena Mostafa Kamal; Ki-Hyun Kim; Kwang-Hyun Shin; Da-Eun Kim; Myeong-Won Oh; Jong-Soon Choi; Hisashi Hirano; Hwa-Young Heo; Sun-Hee Woo
Molecular Biology Reports | 2014
Da-Eun Kim; Swapan Kumar Roy; Abu Hena Mostafa Kamal; Kun Cho; Soo Jeong Kwon; Seong-Woo Cho; Chul-Soo Park; Jong-Soon Choi; Setsuko Komatsu; Moon-Soon Lee; Sun-Hee Woo
한국토양비료학회 학술발표회 초록집 | 2014
Da-Eun Kim; Abu Hena Mostafa Kamal; Soo-Jeong Kwon; Jong-Ho Yang; Ki-Hyun Kim; Seong-Woo Cho; Chul-Soo Park; Moon-Soon Lee; Sun-Hee Woo
한국작물학회 학술발표대회 논문집 | 2011
A-Young Jeon; Abu Hena Mostafa Kamal; Gyong-A Lee; Da-Eun Kim; Myeong-Won Oh; Jin Yul; Ki-Hyeon Kim; Geum-Sook Hwang; Ran-Sun Hong; Yoon-Sup So; Beom-Heon Song; Sun-Hee Woo
한국작물학회 학술발표대회 논문집 | 2011
Myeong-Won Oh; Mohammad-Zaman Nouri; Da-Eun Kim; Jin Yu; Hong-Sig Kim; Setsuko Komatsu; Sun-Hee Woo
한국작물학회 학술발표대회 논문집 | 2011
Myeong-Won Oh; Abu Hena Mostafa Kamal; Da-Eun Kim; A-Young Jeon; Jin Yu; Kun Cho; Cheol-Soo Park; Hong-Sig Kim; Sun-Hee Woo
한국작물학회 학술발표대회 논문집 | 2011
Abu Hena Mostafa Kamal; Kun Cho; Da-Eun Kim; Beom-Heon Song; Young-Gu Cho; Nobuyuki Uozumi; Keun-Yook Chung; Sang-Young Lee; Jong-Soon Choi; Sun-Hee Woo
한국작물학회 학술발표대회 논문집 | 2011
Da-Eun Kim; Abu Hena Mostafa Kamal; Kun Cho; Jin Yu; Myeong-Won Oh; Cheol-Soo Park; Yoon-Sup So; Cheol-won Lee; Sun-Hee Woo