大賀 祥治

Evaluation of arbuscular mycorrhizal fungi using 18S rDNA sequence from root of Botrychium ternatum

The symbiosis with the arbuscular mycorrhiza (AM) was observed in the roots of Botrychium ternatum. The fungal partial 18S ribosomal DNA (rDNA: approximately 450/1800 bp) were amplified from the DNA extracts of fern roots using fungal specific primer, and subcloned to cloning vector (pGEM-T). According to the RFLP pattern of subclone, some clones were selected and their inserts were sequenced. One of them (GLA-006) was predominant in this analysis, however, four (or potentially five) different clone were found in the root of B. ternatum. To infer the phylogenetic relationship, sequences were donated on BLAST Search. According to the result of BLAST Search, neighbor joining analysis was performed. The partial sequencing data of 18S rDNA indicate that these isolates closely related to genus Glomus (AM fungi), especially G. proliferum and G. sinuosum. Three of them were 92-95 % identical to G. proliferum.