Dag E. S. Campbell

Absorption, metabolism, and excretion of salicylazosulfapyridine in man.

Salicylazosulfapyridine (SASP), 4 Gm. daily, was ingested by 9 healthy subfects for 10 days, and the serum concentrations and urinary and fecal excretion of the parent drug and its metabolites were studied. SASP is extensively metabolized by reductive cleavage of the azo linkage, presumably by the action of the gut flora. The sulfapyridine moiety thus formed is subfect to N4‐acetylation or ring hydroxylation followed by confugation to glucuronic acid, or both. The 5‐aminosalicylic acid moiety is N‐acetylated. The steady‐state serum concentrations of the parent drug and its metabolites, obtained by Day 5, for SASP ranged from 4.7 to 45 p.g per milliliter (median 12 p.g per milliliter), for total sulfapyridine metabolites from 37 to 92 p.g per milliliter (median 50 p.g per milliliter), and for total 5‐aminosalicylic acid metabolites to less than 2 p.g per milliliter. The urinary excretion of unchanged SASP ranged from 1.7 to 10 per cent of the dose. About 80 per cent of the dose was excreted in the urine as metabolites of sulfapyridine and one third of the dose was excreted as the metabolite of 5‐aminosalicylic acid. Feces did not contain any SASP, but about 5 per cent of the dose was present as metabolites of sulfapyridine and an unknown amount as metabolites of 5‐aminosalicylic acid.

Quantification of the Inflammatory Reaction and Collagen Accumulation in an Experimental Model of Open Wounds in the Rat: A Methodological Study

An experimental model for studying the early healing of open wounds in the rat is described. With this model, exudate is easily collected, a standardized granulation tissue is achieved at different post-wounding time intervals and local treatment of the wounds is possible. The model involves the use of stainless steel rings with covers, fastened to the edges of two circular full-thickness open wounds, one on each side of the animals back in the thoracic region. Blood flow and water content of the granulation tissue were determined acutely 3, 5, 7 and 10 days after wounding. The collagen accumulation in the granulation tissue was assessed by the amount of hydroxyproline, at the same time intervals. The amount of exudate which accumulated in the chamber was measured daily. Blood flow in the granulation tissue, as measured by radioactive microspheres, reached a maximum of 947 ml X min-1 X (100 g dry weight)-1 7 days after wounding. The water content, as assessed by freeze-drying, also reached its peak on the 7th day (5.5 ml X (g dry weight)-1). Changes in water content were found to be due to changes in interstitial fluid volume, as studied by 59Fe-labelled erythrocytes and 51Cr-EDTA. The amount of hydroxyproline in the granulation tissue increased from 22 micrograms X (mg dry weight)-1 on day 3 to 37 micrograms X (mg dry weight)-1 on day 10. Exudation increased to 28 microliters X h-1 X cm2-1 on day 4 and then declined. The results suggest that the inflammatory reaction, expressed as alterations in blood flow and water content, reaches a maximum on day 7 after wounding. The data also demonstrate a continuous increase in the collagen content of the granulation tissue during the 10-day period of observation. In addition, exudation was found to reach a peak on the 4th to 5th day, and then declined.

Processing of data from an automatic data log

Abstract The administrative procedures for recording instrumental measurement data with a data log system using magnetic tape as recording medium is described. The program for computer sorting, checking and calculation of data is presented.

Automatic data collection for computer calculation of instrument measurements in research laboratories

Abstract An automatic data collecting system (Datalog) was needed in order to rationalize the routine work with different kinds of mainly chemical instruments. A survey of commercially available data logging systems showed that none could directly fulfil our requirements. This prompted a development work, resulting in a data logging system suited for use in the research laboratories of a pharmaceutical company. Fifty four instruments can be connected to the datalog and the instrument measurement value is recorded every 6 sec on magnetic tape for subsequent computer treatment. The recording on the tape is governed from each instrument. This does not interfere with measurements from the other instruments connected to the data log.