Daiana Patrícia Marchetti Pio

Nasal colonization with Staphylococcus aureus in individuals with HIV/ AIDS attended in a Brazilian Teaching Hospital

OBJECTIVE to evaluate the prevalence of nasal colonization with Staphylococcus aureus in individuals with HIV/AIDS under inpatient treatment in a teaching hospital in the state of São Paulo (Brazil). METHOD a cross-sectional study undertaken in two units specialized in attending people living with HIV/AIDS, in the period August 2011 - July 2012. Socio-demographic and clinical data was collected through individual interviews and from the medical records; samples of nasal secretion were collected with Stuart swabs on the first day of inpatient treatment. Ethical aspects were respected. RESULT of the 229 individuals with HIV/AIDS hospitalized in this period, 169 participated in the study, with Staphylococcus aureus being identified in the culture tests of 46 (27.2%) of the individuals, resistance to oxacillin being evidenced in 10 (21.8%) participants. CONCLUSION the results of the research indicate that the prevalence of colonization with Staphylococcus aureus in individuals with HIV/AIDS in the specialized units was considered relevant, possibly contributing to future investigations and, moreover, to the implementation of measures to prevent and control this pathogen in this population.OBJETIVO: avaliar a prevalencia da colonizacao nasal por Staphylococcus aureus em individuos com HIV/Aids internados em um hospital-escola do Estado de Sao Paulo (Brasil). METODO: estudo de corte transversal, realizado em duas unidades especializadas no atendimento a pessoas que vivem com HIV/ Aids, no periodo de agosto 2011 a julho 2012. Foram coletados dados sociodemograficos e clinicos, por entrevista individual e prontuario; as amostras de secrecao nasal foram coletadas por meio de swab Stuart no primeiro dia de internacao. Os aspectos eticos foram contemplados. RESULTADOS: dos 229 individuos com HIV/Aids internados no periodo, 169 participaram do estudo, sendo identificado Staphylococcus aureus nos exames de cultura de 46 (27,2%) dos individuos, evidenciando-se resistencia a oxacilina em 10 (21,8%) participantes. CONCLUSAO: os resultados da pesquisa apontam que a prevalencia da colonizacao por Staphylococcus aureus em individuos com HIV/Aids internados nas unidades especializadas foi considerada relevante, podendo contribuir para novas investigacoes e, ainda, para implementar medidas de prevencao e de controle desse patogeno nessa populacao.

Colonização nasal em profissionais de enfermagem de unidades especializadas em HIV/aids

OBJECTIVE to investigate the presence of microorganisms in the nostrils of the nursing professionals of a Brazilian teaching hospital. METHOD cross-sectional study in two inpatient units specialized in HIV/AIDS. Nasal secretion samples of nursing professionals were collected in one month. The samples were processed at the microbiology laboratory of the institution and analyzed using the Statistical Package for the Social Sciences (SPSS) software, version 19.0. Ethical aspects were abided. RESULTS from the 73 members of the nursing staff, samples of nasal secretions were collected from 61 (80.2%). Six types of microorganisms were isolated in 22 (41.0%) positive cultures. It is noteworthy that Staphylococcus aureus accounted for 22.9%, four of them oxacillin-resistant (MRSA). CONCLUSION Staphylococcus aureus microorganism accounted for the largest prevalence in individuals of this study.

Nasal colonization in nursing professionals from units specialized in HIV/AIDS

OBJECTIVE to investigate the presence of microorganisms in the nostrils of the nursing professionals of a Brazilian teaching hospital. METHOD cross-sectional study in two inpatient units specialized in HIV/AIDS. Nasal secretion samples of nursing professionals were collected in one month. The samples were processed at the microbiology laboratory of the institution and analyzed using the Statistical Package for the Social Sciences (SPSS) software, version 19.0. Ethical aspects were abided. RESULTS from the 73 members of the nursing staff, samples of nasal secretions were collected from 61 (80.2%). Six types of microorganisms were isolated in 22 (41.0%) positive cultures. It is noteworthy that Staphylococcus aureus accounted for 22.9%, four of them oxacillin-resistant (MRSA). CONCLUSION Staphylococcus aureus microorganism accounted for the largest prevalence in individuals of this study.

Colonização nasal por Staphylococcus aureus em indivíduos com HIV/Aids atendidos em um hospital-escola brasileiro

OBJECTIVE to evaluate the prevalence of nasal colonization with Staphylococcus aureus in individuals with HIV/AIDS under inpatient treatment in a teaching hospital in the state of São Paulo (Brazil). METHOD a cross-sectional study undertaken in two units specialized in attending people living with HIV/AIDS, in the period August 2011 - July 2012. Socio-demographic and clinical data was collected through individual interviews and from the medical records; samples of nasal secretion were collected with Stuart swabs on the first day of inpatient treatment. Ethical aspects were respected. RESULT of the 229 individuals with HIV/AIDS hospitalized in this period, 169 participated in the study, with Staphylococcus aureus being identified in the culture tests of 46 (27.2%) of the individuals, resistance to oxacillin being evidenced in 10 (21.8%) participants. CONCLUSION the results of the research indicate that the prevalence of colonization with Staphylococcus aureus in individuals with HIV/AIDS in the specialized units was considered relevant, possibly contributing to future investigations and, moreover, to the implementation of measures to prevent and control this pathogen in this population.OBJETIVO: avaliar a prevalencia da colonizacao nasal por Staphylococcus aureus em individuos com HIV/Aids internados em um hospital-escola do Estado de Sao Paulo (Brasil). METODO: estudo de corte transversal, realizado em duas unidades especializadas no atendimento a pessoas que vivem com HIV/ Aids, no periodo de agosto 2011 a julho 2012. Foram coletados dados sociodemograficos e clinicos, por entrevista individual e prontuario; as amostras de secrecao nasal foram coletadas por meio de swab Stuart no primeiro dia de internacao. Os aspectos eticos foram contemplados. RESULTADOS: dos 229 individuos com HIV/Aids internados no periodo, 169 participaram do estudo, sendo identificado Staphylococcus aureus nos exames de cultura de 46 (27,2%) dos individuos, evidenciando-se resistencia a oxacilina em 10 (21,8%) participantes. CONCLUSAO: os resultados da pesquisa apontam que a prevalencia da colonizacao por Staphylococcus aureus em individuos com HIV/Aids internados nas unidades especializadas foi considerada relevante, podendo contribuir para novas investigacoes e, ainda, para implementar medidas de prevencao e de controle desse patogeno nessa populacao.

Quality of life of elderly people living with HIV/AIDS in outpatient follow-up

OBJECTIVE To analyze factors related to the quality of life of elderly people living with HIV/AIDS. METHOD A cross-sectional study was carried out with people aged 50 years or more in a specialized outpatient clinic. The data collection was by means of an interview. For the analysis of data and characterization of the sample, descriptive statistics and comparison tests were used. The project met the ethical requirements. RESULTS Participants were 81 users aged 50 to 75 years, mean age was 57.8 (± 6.1) years, 71.6% of whom were men. There was a statistically significant relationship with the quality of life, the following variables: gender, children, occupation, religion, diagnosis time, HIV exposure, adverse effects, treatment interruption, viral load counts, hospitalization, dependence for daily activities and use of drugs. CONCLUSION The results suggest that the quality of life deficit is related not only to physical changes, but to the anguish and stigma related to HIV/AIDS.

STAPHYLOCOCCUS AUREUS IN NURSING PROFESSIONALS AND THE MICROORGANISM’S SUSCEPTIBILITY PROFILE TO ANTIMICROBIALS

ABSTRACTObjective: to identify the carrier’s state and the susceptibility profile of Staphylococcus aureus isolated from saliva and nasal secretion of nursing professionals to antibiotics. Method: cross-sectional study that used saliva and nasal secretion samples of 100 nursing professionals who provide care for patients with HIV/Aids. Results: forty-three percent of the participants presented positive saliva or nasal secretion samples for Staphylococcus aureus . Of the 74 nasal secretion samples with Staphylococcus aureus , 14.9% presented oxacillin resistance; 91.9% presented penicillin resistance; 44.6% presented erythromycin resistance, and 41.9% presented clindamycin resistance. Of the 12 positive saliva samples, 16.7% presented oxacillin resistance; 100.0% presented penicillin resistance; 33.4% presented erythromycin resistance, and 25.0% presented clindamycin resistance. Conclusion: nursing professionals, once aware of their carrier state of multi-resistant microorganisms, will supervise their care practices and more efficiently adopt measures for prevention and control of the epidemiological chain of these bacteria in their work environment.

STAPHYLOCOCCUS AUREUS EM PROFISSIONAIS DE ENFERMAGEM E O PERFIL DE SUSCETIBILIDADE DO MICRORGANISMO AOS ANTIMICROBIANOS

ABSTRACTObjective: to identify the carrier’s state and the susceptibility profile of Staphylococcus aureus isolated from saliva and nasal secretion of nursing professionals to antibiotics. Method: cross-sectional study that used saliva and nasal secretion samples of 100 nursing professionals who provide care for patients with HIV/Aids. Results: forty-three percent of the participants presented positive saliva or nasal secretion samples for Staphylococcus aureus . Of the 74 nasal secretion samples with Staphylococcus aureus , 14.9% presented oxacillin resistance; 91.9% presented penicillin resistance; 44.6% presented erythromycin resistance, and 41.9% presented clindamycin resistance. Of the 12 positive saliva samples, 16.7% presented oxacillin resistance; 100.0% presented penicillin resistance; 33.4% presented erythromycin resistance, and 25.0% presented clindamycin resistance. Conclusion: nursing professionals, once aware of their carrier state of multi-resistant microorganisms, will supervise their care practices and more efficiently adopt measures for prevention and control of the epidemiological chain of these bacteria in their work environment.

STAPHYLOCOCCUS AUREUS EN PROFESIONALES DE ENFERMERIA Y EL PERFIL DE SUSCEPTIBILIDAD DE LOS MICROORGANISMOS A LOS ANTIMICROBIANOS

ABSTRACTObjective: to identify the carrier’s state and the susceptibility profile of Staphylococcus aureus isolated from saliva and nasal secretion of nursing professionals to antibiotics. Method: cross-sectional study that used saliva and nasal secretion samples of 100 nursing professionals who provide care for patients with HIV/Aids. Results: forty-three percent of the participants presented positive saliva or nasal secretion samples for Staphylococcus aureus . Of the 74 nasal secretion samples with Staphylococcus aureus , 14.9% presented oxacillin resistance; 91.9% presented penicillin resistance; 44.6% presented erythromycin resistance, and 41.9% presented clindamycin resistance. Of the 12 positive saliva samples, 16.7% presented oxacillin resistance; 100.0% presented penicillin resistance; 33.4% presented erythromycin resistance, and 25.0% presented clindamycin resistance. Conclusion: nursing professionals, once aware of their carrier state of multi-resistant microorganisms, will supervise their care practices and more efficiently adopt measures for prevention and control of the epidemiological chain of these bacteria in their work environment.

Hospitalization of people 50 years old or older living with HIV/AIDS.

Objective Identify the rate and predictive factors of the hospitalization of people living with HIV/AIDS (PLHA), aged 50 years or older. Method A quantitative, cross-sectional study was conducted at two inpatient units specialized in infectious diseases in a teaching hospital. Data were gathered through individual interviews between August 2011 and February 2015. All ethical precepts were followed. Results Of the 532 admitted patients, 95 were PLHA 50 years old or older; 30.5% were admitted 3 to 4 times after being diagnosed with HIV/AIDS. Conclusion Rate of hospitalization was 17.8%, and being 50 to 60 years old was a protective factor against hospitalization.

Staphylococcus aureus and the oxacillin sensitivity profile in hospitalized people with HIV/AIDS

Objective Analyze nasal colonization by oxacillin-sensitive and oxacillin-resistant Staphylococcus aureus in people with HIV/AIDS (PWHA) at days 1 and7 of hospitalization. METHOD A prospective observational study conducted in a hospital in the countryside of the state of São Paulo. Nasal swab samples were collected and analyzed through microbiological identification, at days 1 and 7 of hospitalization of PWHA, between August 2011 and January 2014. Data were analyzed via IBM SPSS(r), version 20.0. RESULTS Nasal secretion samples were collected from 187 (50.1%) PWHA at days 1 and 7 of hospitalization. Of these, Staphylococcus aureus was identified in 64 (34.2%) PWHA. CONCLUSION At day 1 of hospitalization, 27 PWHA were identified with Staphylococcus aureus; 27 PWHA presented colonization by Staphylococcus aureus at days 1 and 7, and 10 PWHA only at day 7. Of 64 PWHA colonized by Staphylococcus aureus, the susceptibility profile of isolated Staphylococcus aureus was oxacillin-resistant in 25 PWHA. OBJETIVO Analisar a colonização nasal por Staphylococcus aureus sensíveis e resistentes à oxacilina de pessoas vivendo com HIV/aids (PVHA) no primeiro e no sétimo dia de internação hospitalar. MÉTODO Estudo prospectivo observacional realizado em um hospital do interior paulista. Foram coletadas e analisadas, por meio de identificação microbiológica, amostras de swab nasal no primeiro e no sétimo dia de internação hospitalar de PVHA, no período de agosto/2011 e janeiro/2014. A análise dos dados foi realizada por meio do IBM SPSS(r), versão 20.0. RESULTADOS Em 187 (50,1%) PVHA foram coletadas amostras de secreção nasal no primeiro e sétimo dia de internação. Destas, em 64 (34,2%) foi identificado Staphylococcus aureus. CONCLUSÃO No primeiro dia de internação observou-se 27 PVHA colonizadas por Staphylococcus aureus; em 27 PVHA houve a persistência da colonização por Staphylococcus aureus no primeiro e no sétimo dia; em 10 PVHA, somente no sétimo dia. Das 64 PVHA colonizadas por Staphylococcus aureus, em 25 o perfil de suscetibilidade do Staphylococcus aureus isolado foi resistente à oxacilina.www.ee.usp.br/reeusp Rev Esc Enferm USP · 2016;50(4):617-621 1 Universidade de São Paulo, Escola de Enfermagem de Ribeirão Preto, Programa Interunidades de Pós-Graduação em Enfermagem, Ribeirão Preto, SP, Brasil. 2 Universidade de São Paulo, Escola de Enfermagem de Ribeirão Preto, Programa de Pós-Graduação em Enfermagem Fundamental, Ribeirão Preto, SP, Brasil. 3 Universidade de São Paulo, Escola de Enfermagem de Ribeirão Preto, Departamento de Enfermagem Geral e Especializada, Ribeirão Preto, SP, Brasil. ABSTRACT Objective: Analyze nasal colonization by oxacillin-sensitive and oxacillin-resistant Staphylococcus aureus in people with HIV/AIDS (PWHA) at days 1 and7 of hospitalization. Method: A prospective observational study conducted in a hospital in the countryside of the state of São Paulo. Nasal swab samples were collected and analyzed through microbiological identification, at days 1 and 7 of hospitalization ofPWHA, between August 2011 and January 2014. Data were analyzed via IBM SPSS®, version 20.0. Results: Nasal secretion samples were collected from 187 (50.1%) PWHA at days 1 and 7 of hospitalization. Of these, Staphylococcus aureus was identified in 64 (34.2%) PWHA. Conclusion: At day 1 of hospitalization, 27 PWHA were identified with Staphylococcus aureus; 27 PWHA presented colonization by Staphylococcus aureus at days 1 and 7, and 10 PWHA only at day 7. Of 64 PWHA colonized by Staphylococcus aureus, the susceptibility profile of isolated Staphylococcus aureus was oxacillin-resistant in 25 PWHA.