Daisuke Fukuta
Osaka University
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Featured researches published by Daisuke Fukuta.
Journal of Biological Chemistry | 1997
Keiko Hayami; Daisuke Fukuta; Yasuhiro Nishikawa; Yumi Yamashita; Masanori Inui; Yukiya Ohyama; Masaki Hikida; Hitoshi Ohmori; Toshiyuki Takai
We have isolated a cDNA clone encoding a novel murine cell-surface glycoprotein. This polypeptide is predicted to be composed of a signal peptide of 23 amino acids, an extracellular region of 620 amino acids that contains six immunoglobulin-like domains with five potential N-glycosylation sites, a transmembrane sequence of 20 amino acids, and a cytoplasmic tail of 178 amino acids with four sets of sequences similar to the immunoreceptor tyrosine-based inhibition motif. The relative molecular mass of the mature polypeptide is calculated to be 90,520 Da. The polypeptide, designated as p91, shows striking homologies to human killer cell inhibitory receptors, a murine gp49B1 protein, a bovine Fcγ2 receptor, and a human Fcα receptor. The mRNA of p91 was especially abundant in murine macrophages. Western blot analysis using p91-specific anti-peptide sera detected a 130-kDa polypeptide in macrophages. Surface biotinylation and immunoprecipitation analysis verified the surface expression of the translation products on COS-1 cells transfected with the p91 cDNA, but the cells failed to show any Fc binding activity.
Journal of Immunology | 2004
Shuji Miyagawa; Tomoko Kubo; Katsuyoshi Matsunami; Tamiko Kusama; Keiko Beppu; Hiroshi Nozaki; Toshiyuki Moritan; Curie Ahn; Jae Young Kim; Daisuke Fukuta; Ryota Shirakura
NK cells play a critical role in the rejection of xenografts. In this study, we report on an investigation of the effect of complement regulatory protein, a decay accelerating factor (DAF: CD55), in particular, on NK cell-mediated cytolysis. Amelioration of human NK cell-mediated pig endothelial cell (PEC) and pig fibroblast cell lyses by various deletion mutants and point substitutions of DAF was tested, and compared with their complement regulatory function. Although wild-type DAF and the delta-short consensus repeat (SCR) 1-DAF showed clear inhibition of both complement-mediated and NK-mediated PEC lyses, delta-SCR2-DAF and delta-SCR3-DAF failed to suppress either process. However, delta-SCR4-DAF showed a clear complement regulatory effect, but had no effect on NK cells. Conversely, the point substitution of DAF (L147·F148 to SS and KKK125–127 to TTT) was half down-regulated in complement inhibitory function, but the inhibition of NK-mediated PEC lysis remained unchanged. Other complement regulatory proteins, such as the cell membrane-bound form factor H, fH-PI, and C1-inactivator, C1-INH-PI, and CD59 were also assessed, but no suppressive effect on NK cell-mediated PEC lysis was found. These data suggest, for DAF to function on NK cells, SCR2–4 is required but no relation to its complement regulatory function exists.
Xenotransplantation | 2003
Daisuke Fukuta; Shuji Miyagawa; M Yamada; Katsuyoshi Matsunami; Takashi Kurihara; Akio Shirasu; Hiroyuki Hattori; Ryota Shirakura
Fukuta D, Miyagawa S, Yamada M, Matsunami K, Kurihara T, Shirasu A, Hattori H, Shirakura R. Effect of various forms of the C1 esterase inhibitor (C1‐INH) and DAF on complement mediated xenogeneic cell lysis. Xenotransplantation 2003; 10: 132–141.
Biochemical and Biophysical Research Communications | 2003
Daisuke Fukuta; Shuji Miyagawa; Tomoko Kubo; Katsuyoshi Matsunami; Akio Shirasu; Hiroyuki Hattori; Ryota Shirakura
To suppress C3 fragment deposition in the classical pathway complement activation on xenogeneic membranes, decay accelerating factor (DAF) was the most effective molecule among the complement regulatory proteins (CRPs) used in the present study. C3 fragment deposition was closely related to subsequent xenogeneic cell lysis. However, other molecules were also very effective in different ways and include phosphatidylinositol (PI)-anchored short consensus repeat (SCR) 2-4 of membrane cofactor protein (MCP-PI), PI-anchored C1 esterase inhibitor (C1-INH-PI), and PI-anchored SCR8-11 of complement receptor type 1 (CR1-PI). On the other hand, regarding a strategy for downregulating C4 fragment deposition, the use of only C1-INH-PI and PI-anchored SCR1-3 of the C4b-binding protein (C4bp-PI) was found to be effective.
Xenotransplantation | 2006
Shuji Miyagawa; Daisuke Fukuta; Etsuko Kitano; Chizuko Kobayashi; Yuichi Fumimoto; Akio Shirasu; Hiroyuki Hattori; Ryota Shirakura; Masahiro Fukuzawa
Abstract: Background: It is difficult to produce a transgenic animal with high expression of decay‐accelerating factor (CD55: DAF) or other molecules. The purpose of this study was to assess the effect of tandem forms of DAF on a xenogeneic cell membrane against human complement.
Journal of Biochemistry | 1998
Yumi Yamashita; Daisuke Fukuta; Atsushi Tsuji; Akira Nagabukuro; Yoichi Matsuda; Yasuhiro Nishikawa; Yukiya Ohyama; Hitoshi Ohmori; Masao Ono; Toshiyuki Takai
Transplantation Proceedings | 2003
Tamiko Kusama; Shuji Miyagawa; Toshiyuki Moritan; T Kubo; M Yamada; H Sata; Daisuke Fukuta; Katsuyoshi Matsunami; R Shirakura
Transplant Immunology | 2003
Daisuke Fukuta; Shuji Miyagawa; Tomoko Kubo; Tamiko Kusama; Akio Shirasu; Hiroyuki Hattori; Ryota Shirakura
Journal of Biochemistry | 2002
Katsuyoshi Matsunami; Shuji Miyagawa; Daisuke Fukuta; Ryota Shirakura
Transplantation Proceedings | 2003
Daisuke Fukuta; Shuji Miyagawa; M Yamada; Katsuyoshi Matsunami; Takashi Kurihara; Akio Shirasu; H Hattori; R Shirakura