Dallas E. Peterson

Interference of redroot pigweed (Amaranthus retroflexus), Palmer amaranth (A. palmeri), and common waterhemp (A. rudis) in soybean

Abstract Field studies were conducted in 1997 and 1998 at Manhattan and Topeka, KS, to examine the competitive effects of redroot pigweed, Palmer amaranth, and common waterhemp on soybean yield. The experiments were established as a randomized complete block design in a factorial arrangement of three pigweed species, two pigweed planting dates (soybean planting and cotyledon stage), and seven weed densities (0.25, 0.5, 1, 2, 4, and 8 plants m−1 of row, plus a weed-free control). The effect of weed density on soybean yield loss, pigweed biomass, and pigweed seed production were described using a rectangular hyperbola model. Soybean yield loss varied between locations depending on the weed species, density, and time of emergence. Yield loss increased with weed density for each species and location with the first pigweed emergence time. The maximum soybean yield loss occurred at the first planting and 8 plants m−1 of row density, and was 78.7, 56.2, and 38.0% as determined by the model for Palmer amaranth, common waterhemp, and redroot pigweed, respectively. The second planting of pigweed did not significantly reduce soybean yield. The relative ranking of the pigweed species biomass was Palmer amaranth > common waterhemp > redroot pigweed. Maximum seed production for Palmer amaranth, common waterhemp, and redroot pigweed was 32,300, 51,800, and 9,500 seeds m−2. Palmer amaranth produced a larger quantity of seed than did common waterhemp or redroot pigweed at low weed densities (0.25 to 4 plants m−1 of row). But common waterhemp seed production equaled or surpassed Palmer amaranth at high weed densities. Nomenclature: Palmer amaranth, Amaranthus palmeri S. Wats. AMAPA; common waterhemp, Amaranthus rudis Sauer AMATA; redroot pigweed, Amaranthus retroflexus L. AMARE; soybean, Glycine max (L.) Merr.

Common waterhemp (Amaranthus rudis) resistance to protoporphyrinogen oxidase-inhibiting herbicides

Abstract Resistance to protoporphyrinogen oxidase (protox)-inhibiting herbicides was identified in a population of common waterhemp that had been treated with acifluorfen for several years. The protox-resistant biotype of common waterhemp was approximately 34, 82, 8, and 4 times more resistant than a susceptible common waterhemp biotype to acifluorfen, lactofen, fomesafen, and sulfentrazone, respectively. The resistant biotype also showed a high level of resistance to acetolactate synthase–inhibiting herbicides thifensulfuron and imazethapyr but not to glyphosate or paraquat. An organophosphate insecticide was applied with acifluorfen or lactofen to determine if metabolism could be the mechanism of resistance. No differences were observed between resistant plants treated with an organophosphate plus a protox-inhibiting herbicide and plants treated with a protox-inhibiting herbicide alone. Nomenclature: Acifluorfen-methyl; lactofen; fomesafen; sulfentrazone; thifensulfuron-methyl; imazethapyr; glyphosate; paraquat; malathion; diazinon; common waterhemp, Amaranthus rudis Sauer AMATA.

Efficacy of glyphosate, glufosinate, and imazethapyr on selected weed species

Abstract Experiments were conducted to determine the efficacy, absorption, and translocation of glyphosate, glufosinate, and imazethapyr with selected weed species. In the greenhouse glyphosate, glufosinate, and imazethapyr were applied at 0.25, 0.5, and 1 times their label rates of 1,121, 410, and 70 g ha−1, respectively, on 10- to 15-cm black nightshade, common waterhemp, eastern black nightshade, field bindweed, giant ragweed, ivyleaf morningglory, prairie cupgrass, velvetleaf, and yellow nutsedge. Glyphosate applied at the 1-time rate caused injury greater than or similar to injury from the 1-time rate of glufosinate or imazethapyr on black nightshade, common waterhemp, eastern black nightshade, field bindweed, giant ragweed, prairie cupgrass, and velvetleaf. The 1-time rate of glufosinate injured ivyleaf morningglory and yellow nutsedge more than did the 1-time rate of glyphosate or imazethapyr. Under field conditions glyphosate caused the greatest injury to common waterhemp, prairie cupgrass, and velvetleaf across plant growth stages. Giant ragweed and ivyleaf morningglory injury was more dependent on growth stage, with the 15- and 30-cm growth stages more susceptible to glyphosate than to glufosinate or imazethapyr. Differential response of these weed species may be caused by differences in herbicide translocation. Glyphosate was translocated more in both giant ragweed and ivyleaf morningglory, and these species were injured more by glyphosate than by glufosinate or imazethapyr at the larger growth stages. Nomenclature: Glufosinate; glyphosate; imazethapyr; black nightshade, Solanum nigrum L. SOLNI; common waterhemp, Amaranthus rudis Sauer AMATA; eastern black nightshade, Solanum ptycanthum Dun. SOLPT; field bindweed, Convolvulus arvensis L. CONAR; giant ragweed, Ambrosia trifida L. AMBTR; ivyleaf morningglory, Ipomoea hederacea (L.) Jacq. IPOHE; prairie cupgrass, Eriochloa contracta Hitchc. ERICO; velvetleaf, Abutilon theophrasti Medicus ABUTH; yellow nutsedge, Cyperus esculentus L. CYPES.

Glufosinate Efficacy on Amaranthus Species in Glufosinate-Resistant Soybean (Glycine max)1

Field studies were conducted in 1998 and 1999 to evaluate the efficacy of glufosinate on Palmer amaranth, redroot pigweed, and common waterhemp at different growth stages in soybean planted at early, normal, and late dates. At 2, 4, and 8 wk after treatment, common waterhemp control was greater than Palmer amaranth and redroot pigweed control with single glufosinate applications of 410 g ai/ha at 2- to 5-, 7- to 10-, or 15- to 18-cm Amaranthus height or with two sequential applications of 293 g/ha at 2- to 5-cm height and 2 wk later. Only the sequential applications of 410 and 293 g/ha resulted in more than 80% control of Palmer amaranth and redroot pigweed, but all five treatments controlled common waterhemp more than 80%. All glufosinate treatments reduced the dry weight of all Amaranthus species by more than 65%. However, the sequential applications resulted in the greatest dry weight reductions. Nomenclature: Glufosinate; common waterhemp, Amaranthus rudis Sauer #3 AMATA; Palmer amaranth, Amaranthus palmeri S.Wats. # AMAPA; redroot pigweed, Amaranthus retroflexus L. # AMARE; soybean, Glycine max (L.) Merr. Additional index words: Environmental conditions, herbicide-tolerant soybean, postemergence herbicide. Abbreviations: ALS, Acetolactate synthase (EC 4.1.3.18); OM, organic matter; RH, relative humidity; WAT, weeks after treatment.

Differential Kochia (Kochia scoparia) Populations Response to Glyphosate

Abstract Kochia is a troublesome weed throughout the western United States. Although glyphosate effectively controls kochia, poor control was observed in several no-till fields in Kansas. The objectives of this research were to evaluate kochia populations response to glyphosate and examine the mechanism that causes differential response to glyphosate. Glyphosate was applied at 0, 54, 109, 218, 435, 870, 1305, 1740, 3480, and 5220 g ae ha−1 on 10 kochia populations. In general, kochia populations differed in their response to glyphosate. At 21 d after treatment, injury from glyphosate applied at 870 g ha−1 range from 4 to 91%. In addition, glyphosate rate required to cause 50% visible injury (GR50) ranged from 470 to 2149 g ha−1. Differences in glyphosate absorption and translocation and kochia mineral content were not sufficient to explain differential kochia response to glyphosate. Nomenclature: Glyphosate; kochia, Kochia scoparia (L.) Schrad.

Protox-resistant common waterhemp (Amaranthus rudis) response to herbicides applied at different growth stages

Abstract Greenhouse and field studies were conducted with a population of common waterhemp resistant to POST protoporphyrinogen oxidase (protox)-inhibiting herbicides to compare its response to PRE and POST applications of selected herbicides. In the greenhouse, a dose–response study of PRE applications of acifluorfen, fomesafen, or lactofen was conducted on protox-susceptible and -resistant common waterhemp. The protox-resistant biotype was approximately 6.3, 2.5, and 2.6 times more resistant than the susceptible biotype to acifluorfen, fomesafen, and lactofen, respectively. In a separate study under field conditions, protox-resistant common waterhemp were treated with PRE and POST applications of acifluorfen, azafenidin, flumioxazin, fomesafen, lactofen, oxyfluorfen, or sulfentrazone. At 14 and 28 d after POST treatment (DAPT) in 2002 and 2004, all PRE applications of herbicides gave greater control than did POST applications. At 14 DAPT, oxyfluorfen had the greatest difference in PRE and POST control, with 85 and 10% control in 2002, respectively. An additional field study was conducted to determine the stage of growth at which resistance to protox-inhibiting herbicides becomes most prevalent. Protox-resistant common waterhemp were treated with herbicides at the 2-leaf, 4- to 6-leaf, and 8- to 10-leaf growth stage. Acifluorfen and fomesafen at 420 g ha−1 gave greater than 90% control at the 2-leaf stage and 4- to 6-leaf stage, except in 2003 when control was 85% with acifluorfen. In 2003 and 2004, common waterhemp control at the 8- to 10-leaf stage ranged between 54 and 75% with acifluorfen or fomesafen. Results indicate that common waterhemp resistance to customary rates of POST protox-inhibiting herbicides becomes prevalent after the 4- to 6-leaf growth stage. Nomenclature: Acifluorfen; azafenidin; bentazon; flumioxazin; fomesafen; lactofen; oxyfluorfen; sulfentrazone; common waterhemp, Amaranthus rudis Sauer AMATA.

Wheat Response to Simulated Drift of Glyphosate and Imazamox Applied at Two Growth Stages

Field experiments were conducted at Hays and Manhattan, KS, in 2002 and 2003 to determine winter wheat response to simulated drift rates of glyphosate and imazamox. Glyphosate and imazamox at 1/100×, 1/33×, 1/10×, and 1/3× of usage rates of 840 g ae/ha glyphosate and 35 g/ha imzamox were applied individually to wheat in the early jointing or the early flower stages of growth. Wheat injury and yield loss increased as herbicide rate was increased, with minimal effect from either herbicide at the 1/100× rate, and nearly complete kill and yield loss of wheat from both herbicides applied at the 1/3× rate, regardless of growth stage at application. In general, wheat injury and yield reduction were greater from glyphosate than from imazamox. In addition, wheat injury and yield loss were greater from herbicide treatment at the jointing stage than at the flowering stage of development. Correlation analysis suggests that visual injury is an accurate indicator of yield reductions. Germination tests of harvested grain showed that the viability of the wheat seed was not reduced if plants survived the herbicide treatment and produced a harvestable seed. Nomenclature: Glyphosate; imazamox; wheat, Triticum aestivum L. Addition index words: Crop injury, herbicide drift, herbicide symptoms, yield reduction. Abbreviations: WAT, weeks after treatment.

Cotton Injury and Yield as Affected by Simulated Drift of 2,4-D and Dicamba

Abstract Experiments were conducted at Manhattan, KS in 2005 and 2006 to evaluate cotton response to simulated 2,4-D and dicamba drift rates at different stages of growth and multiple applications of 2,4-D. Cotton was treated with 2,4-D and dicamba at 0, 1/200, and 1/400 of the use rate (561 g ae/ha) when plants were at the three- to four-leaf, 8-, 14-, or 18-node growth stages. Injury symptoms after 2,4-D and dicamba application were more severe at the three- to four-leaf stage compared with other stages with greatest injury from 2,4-D. In general, plants partially recovered from 2,4-D and dicamba injury symptoms, and only 2,4-D applied at the 1/200 rate reduced fiber yield. In a separate study, cotton was treated with 2,4-D at 0, 1/400, 1/800, and 1/1,200 of the use rate for one, two, or three applications. Yield reduction increased as herbicide rate increased from 1/1,200 to 1/400 and the number of applications increased from one to three. In both studies, plants partially or fully recovered from injury symptoms and recovery was greater with dicamba than 2,4-D. Correlation coefficient analysis showed that visual injury ratings later in the growing season are a good predictor of yield reduction (R2  =  0.58). Nomenclature: 2,4-D (2,4-dichlorophenoxy) acetic acid; dicamba, 3,6-dichloro-2-methoxybenzoic acid; ethephon, (2-chloroethyl) phosphonic acid; tribufos, S,S,S-tributyl phosphorotrithioate; cotton, Gossypium hirsutum L

Survey of Common Waterhemp (Amaranthus rudis) Response to Protox- and ALS-Inhibiting Herbicides in Northeast Kansas'

A population of common waterhemp in northeast Kansas was confirmed resistant to protoporphyrinogen oxidase (protox)-inhibiting herbicides in 2001. In 2002, seeds were collected from 28 sites in a 16-km radius surrounding the site where resistance was confirmed to determine the extent of protox resistance in common waterhemp populations throughout the area. In addition, common waterhemp response to acetolactate synthase (ALS)-inhibiting herbicides and glyphosate was determined. At least one common waterhemp plant among the 48 plants tested from each of 10 sites was acifluorfen-resistant. These sites were randomly scattered throughout the sampling area, and resistance may have resulted from seed or pollen movement or independent development. Acifluorfen-resistant common waterhemp plants were initially injured by acifluorfen, but plants began recovering from injury within 14 days after treatment (DAT). All sites contained at least two common waterhemp plants with imazethapyr resistance, whereas plants from all sites were susceptible to glyphosate. Because acifluorfen- and imazethapyr-resistant common waterhemp populations are found in northeastern Kansas, protox-inhibiting and ALS-inhibiting herbicides may not provide common waterhemp control. Nomenclature: acifluorfen-methyl; glyphosate; imazethapyr; common waterhemp, Amaranthus rudis Sauer, #3 AMATA. Additional index word: Herbicide resistance. Abbreviations: ALS, acetolactate synthase [E.C. 2.2.1.6]; DAT, days after treatment; protox, protoporphyrinogen oxidase [E.C. 1.3.3.4].

A Multistate Study of the Association Between Glyphosate Resistance and EPSPS Gene Amplification in Waterhemp (Amaranthus tuberculatus)

Abstract Waterhemp is an increasingly problematic weed in the U.S. Midwest, having now evolved resistances to herbicides from six different site-of-action groups. Glyphosate-resistant waterhemp in the Midwest is especially concerning given the economic importance of glyphosate in corn and soybean production. Amplification of the target-site gene, 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) was found to be the mechanism of glyphosate resistance in Palmer amaranth, a species closely related to waterhemp. Here, the relationship between glyphosate resistance and EPSPS gene amplification in waterhemp was investigated. Glyphosate dose response studies were performed at field sites with glyphosate-resistant waterhemp in Illinois, Kansas, Kentucky, Missouri, and Nebraska, and relative EPSPS copy number of survivors was determined via quantitative real-time polymerase chain reaction (qPCR). Waterhemp control increased with increasing glyphosate rate at all locations, but no population was completely controlled even at the highest rate (3,360 g ae ha−1). EPSPS gene amplification was present in plants from four of five locations (Illinois, Kansas, Missouri, and Nebraska) and the proportion of plants with elevated copy number was generally higher in survivors from glyphosate-treated plots than in plants from the untreated control plots. Copy number magnitude varied by site, but an overall trend of increasing copy number with increasing rate was observed in populations with gene amplification, suggesting that waterhemp plants with more EPSPS copies are more resistant. Survivors from the Kentucky population did not have elevated EPSPS copy number. Instead, resistance in this population was attributed to the EPSPS Pro106Ser mutation. Results herein show a quantitative relationship between glyphosate resistance and EPSPS gene amplification in some waterhemp populations, while highlighting that other mechanisms also confer glyphosate resistance in waterhemp. Nomenclature: Glyphosate; common waterhemp, Amaranthus tuberculatus (Moq.) Sauer var. rudis (Sauer) Costea and Tardif; Palmer amaranth, Amaranthus palmeri S. Wats AMAPA; corn, Zea mays L.; soybean, Glycine max (L.) Merr.